正常以下的

By systemic researching and simulating experiment, the text gains such results as follows,1. On the base of sufficiency research on the oil-gas hydrocarbon systemic chemistry composing and the physical chemistry characters, we traverse the mechanism of the paraffin's deposition. Then we consider that paraffin deposition is a complex process ofphysical chemistry, which includes more than one single mechanism.2. Traversing and comparing the literature in detail on different factors that has effect on paraffin deposition, the available experimental methods on paraffin deposition, prevention of paraffin deposition, and removing of the deposited paraffin on the spot.3. Ameliorated the laser equipment of solid particle inspecting system and the corresponding test methods. Determine WCT of dead oil and live oil from KS101 well under different pressure with this system. The result implies that dead oil has opposite regulation on paraffin deposition temperature to live oil.4. A vapor-liquid-solid envelop is drawn by superposition regular vapor-liquid envelop and WCT curve thereby explained that the departure and quick raising of the curve in the low temperature zone is caused by the appearance of paraffin in solid phase.5. Estimation is made to determine the depth of paraffin deposition in the well.

通过系统的研究和实验模拟，本文取得了以下研究成果：(1)在充分调研油气烃类体系的化学组成及其物理化学特征的基础上，详细研究了石蜡沉降的机理，认为石蜡沉积是一个复杂的物理化学过程，是多种机理共同作用的结果；(2)深入调研并综合阐述了影响石蜡固相沉积的各种主要因素，对目前国内外常用的有机固相沉积实验研究方法和现场清防蜡措施也做出详尽的描述，并加以对比评价；(3)改进了油气藏开发重点实验室自行研制组装的高温高压下测定固相沉积的激光装置和测试方法，并用该套设备测定出柯深101井的井口脱气油样和凝析气平衡油样不同压力下的析蜡点，实验结果表明，脱气油和平衡油析蜡规律相反；(4)将常规气-液两相相图和析蜡曲线叠加得到了气—液—固三相相图，从而解释了气-液两相等液量线在低温区域偏离正常并快速上翘是由于体系有石蜡固相出现；(5)利用实验测试的析蜡温度预测析蜡位置，表明只有在距井口465m油管以上会有蜡析出。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。