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Physical and gynecologic examination 20 months earlier had been normal; the Pap smear was normal, with endocervical cells present.

物理和妇科检查20个月早些时候已经正常;巴氏涂片是正常的,与颈管内膜细胞的礼物。

The morphologic change of muscle fibers: At the early stage of denervation, the area of cross sections decreased, some muscle fibers turned into angular fibers, nuclei congregated around the center of the fibers. After 8 weeks of denervation, atrophic and hypertrophic fibers coexist. Collagen increases under the endomysium and between the fibers.

肌纤维的组织形态学变化:面部肌肉失神经支配早期,肌纤维的横截面面积变小,出现角状纤维,肌细胞核聚集,细胞核从肌纤维边缘移至肌纤维中央附近;失神经支配后期,肿胀与萎缩的肌纤维并存,肿胀的肌纤维的横截面成倍大于正常的肌纤维,而萎缩的肌纤维的横截面则成倍小于正常的肌纤维;有大量的角纤维存在,肌纤维之间有较多的结缔组织增生。

Methods: Immunohistochemistry and image analysis techniques are applied to examine the phosphorylated extracellular signal-regulated kinase in epidermis of non-expanded skin and expanded skin. Results:Phosphorylated ERK expressed in basal cell layer of epiderm including non-expanded skin and expanded skin are expressed. But in expanded skin they are expressed significantly and densely compared to non-expanded skin. There are significant difference in relative to location of expanded skin and regions of expanded skin.

结果: 1扩张皮肤和正常皮肤表皮基底层都有磷酸化ERK 的表达和分布,但扩张皮肤中的磷酸化ERK的分布和表达较明显,染色较深且密,部分阳性细胞呈多层排列,有散在的增殖团区;2扩张皮肤的顶部和侧部差别不十分明显,而侧部和顶部与基底部有一定的差别,基底部染色较深,多为细胞核着色;3 经图像分析不同部位的正常皮肤和扩张皮肤以及扩张皮肤的不同部分的相对灰度值和阳性细胞密度,进一步证实了上述观察结果。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数来评估神经元的情况。

Animals were in normal control group,6animals were in normal treated group (tPNS200mg/kg) The others were cauterized three episcleral vessels and were divided as experimental groups: groupA (50mg/kg), groupB(100mg/kg), groupC(150mg/kg), groupD (200mg/kg)and group treated by normal saline.Rat were sacrificed on schedule.

健康SD大鼠42只,其中6只为正常对照组,6只为正常用药组(三七总皂苷200mg/kg),其余30只采Akira法,烙闭大鼠右眼上巩膜静脉,制作大鼠持续性高眼压模型,随机分为生理盐水组,量效关系组分别予tPNS 50mg/kg,100mg/kg,150mg/kg,200mg/kg进行治疗一个月。

The percentage of myeloblasts and promyelocytes of the patients with myelodysplastic syndrome increased when compared with that of controls.Morphological changes including nuclear abnormalities were also observed in granulocytic and erythrocytic lines.

骨髓增生异常综合征患者骨髓中原粒和早幼粒细胞百分率高于正常,分别为5.08和7.85(正常参考值分别为0.64和1.59),粒细胞和红细胞有细胞形态异常和核畸形。

Because the patients′platelets had normal reaction to low concentration (another TXA〓-dependent agent) and these indicated that the common post-receptor pathway of signal transduction was normal when the excitory stimulus combined with the patients′platelets.

由于患者对低浓度胶原(亦为TXA〓依赖的诱聚剂)反应正常,说明患者血小板在与活化信号结合后,其共同的受体后信号传导道路是正常的。

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