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In this research, we extracted the genomic DNA from the Buxus sinica var. parvifolia leaves by using the modified CTAB approach and then used as the template DNA. Base on the invariable quantity of TaqDNA, we att empted to establish and optimize ITS-PCR amplification system for Buxus sinica var. parvifolia by employing L9 (3^4) orthogonal design with four factors, i.e. template DNA, Mg(superscript 2+), dNTPs and primer.

本研究利用改良CTAB法从珍珠黄杨叶片中提取基因组DNA作为模板,在TaqDNA聚合酶量不变的基础上,利用正交设计L9(3^4)对4个因素(模板DNA、Mg(上标2+)、dNTP和引物)在3个水平上对珍珠黄杨ITS-PCR反应体系进行优化。

ObjectiveTo develop an extraction process of organic acids from Capsella buras-pastoris by ultrasonic extraction.

方法采用单因素实验/正交实验,研究了温度、超声波作用时间、料液比、超声波功率对荠菜中有机酸提取量的影响规律。

In this paper, five oxygenic terpenoids —Carveol (C10H160),Carvone (C10H140),Menthone (C10H180),Carvyl acetate (C12H1802) andCedrol (C15H260) were used as test compounds and barnyardgrass( Enchinochloa crusgalli L.) receptor in rotational regression design experiment.

以5种含氧萜类物质香芹醇、香芹酮、薄荷酮、香芹基乙酸酯和雪松醇为化感物质替代物,以稗草为受体,运用正交旋转回归组合实验,分析物质间的互作效应。

The processing conditions of extracting carya cathayensis oil by ultrasonic extraction is studied by orthogonal experiment.

采用正交实验法研究超声波法提取山核桃油的工艺条件。

The influences of plating process parameters, such as sulphamate concentration, electrolyte pH value and temperature, and pulse parameters, such as cathodal current density, pulse current frequency and duty cycle, on the micro-hardness of the nickel deposit has been studied by orthogonal experiments.

利用正交试验法分析研究了主盐氨基磺酸镍浓度、电镀过程工艺参数及脉冲参数(阴极电流密度、脉冲频率和占空比)对镀层显微硬度的影响;并对相关参数进行了优化。

Multiple-walled carbon Nanotubes were used as carrier to load cerous sulfate catalyst for catalytic synthesis of n-butyl propionate Single factor and orthogonal experiment methods were applied to investigate the effects of the molar ratio of butyl alcohol to propionic acid, cerous sulfate mass fraction and reaction time on the conversion of propionic acid and the optimal technical conditions were determined as:ratio of butyl alchhol and propionic acid 1.5:1, cerous sulfate mass fraction 15% and reaction time 70 min.

以多壁碳纳米管作载体担载硫酸铈催化合成丙酸丁酯。分别采用单因素和正交实验方法考察了醇酸摩尔比、硫酸铈质量分率和反应时间等主要因素对丙酸丁酯得率的影响,并确定了最佳工艺条件为:醇酸摩尔比1.5:1、硫酸铈质量分率15%和反应时间70 min。

With C.praecox var concolor as the tested material, 3 kinds of medicaments as Penicillin (700, 900, 1 100 mg/L), AgNO3 (102, 136, 170 mg/L) and 8-HQ (50, 70, 90, 200 mg/L) were used to make the orthogonal test, the fresh-keeping formula that was suitable for cutting-flower of C. praecox was screened and 5 indexes of fresh weight of flower shoots, water balance, blooming rate, soluble sugar content and protein content of C. praecox were determined.

方法]以素心腊梅为试材,以青霉素(700、900、1 100 mg/L)、硝酸银(102、136、170 mg/L)8-羟基喹啉(50、70、90、200 mg/L)3种药剂不同浓度进行正交试验,筛选适于切花腊梅保鲜剂配方,测定切花腊梅的花枝鲜重、水分平衡值、开花率、糖及蛋白质含量5个指标。

The best extraction and purification technology of Coprinus comatus polysaccharide were optimized with the orthogonal design.

本文研究了提取温度、时间、次数和固液比等因素对提取得率的影响,用正交试验优化。

METHODS: Using orthogonal experiments,the adsorptive characteristics and elutive parameters of the process were studied by taking the elutive effects and purification ratio of total flavonoids of Crataegus leaves.

采用正交实验法,以固形物中总黄酮含量为考察指标,对影响黄酮提取工艺的因素进行了研究。

Methods: The saturated water solution mixing method was compared with microwave method and ultrasonic method by determining the ultilization ratio of the volatile oil in Dalbergia odorifera.

以挥发油包合率为评价指标,比较饱和水溶液搅拌法、微波法及超声波法,以正交实验优选最佳工艺条件,并用TLC对包合前后的挥发油性质进行分析。

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推荐网络例句

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性,但我大喊,并在他们的方向扔石头让他们过的线索,远离我,以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。

Store this product in a sealed, lightproof, dry and cool place.

密封,遮光,置阴凉干燥处。