次培养基

The study results indicated that the proliferation and radication of in vitro seedling were made simultaneously, the common-used culture medium was H+NAA 0.15 mg/L and radication rate was 80%~98%.

研究结果表明，组培苗增殖与生根同步进行，通用最佳培养基为：H+NAA0.15mg/L，生根率80%～98%，年增殖次数8次，繁殖系数为5。蛭石粉+泥炭土 1∶1 是三倍体毛白杨组培苗炼苗的最佳基质。

I used my faster speed to reprepare bacteria medium

我提快了配制培养基的速度，大约快22点半的时候开始第二次灭菌！

Quadratic regressive factorial experiment was used to optimize concentration of 6-BA and NAA in MS medium for shoot propagation from the top buds and stem segments of Russelia equisetiformis.

以爆仗竹顶芽与带腋芽的茎段为外植体，采用二次回归正交设计方法研究了爆仗竹组织培养快速繁殖中不定芽增殖系数与继代培养基中6BA和NAA组合及其浓度配比之间的关系。

Optimization of liquid culture medium including maltose, glucose and tryptone for nucleotides production with Cordvceps sinensis CS-wx was carried out by response surface method.

采用响应面法对冬虫夏草茵CS-wx液态发酵生产核苷类物质的3种主要培养基成分——麦芽糖、葡萄糖和蛋白胨进行优化，采用多元二次回归方程拟合3种因素与核苷类物质含量为响应值之间的函数关系。

The functional relationship between the content of pyruvic acid and three factors,including maltose,glucose and tryptone,was fit using multi-power regression function method.

采用响应面法对丙酮酸发酵培养基成分葡萄糖、硫酸铵、蛋白胨进行优化，采用多元二次回归方程拟合3种因素与丙酮酸含量间的函数关系，并得到了最佳条件。

RPMI 1640 medium to induce hyphae at 37℃ for seven days was thought to be the favorable condition for C.

RPMI 1640培养基（P＝7.5），37aC传代培养7天（转种 12次），是获得菌丝相白念珠菌的理想条件； 3。

Can improve the survival ratio of seeding. Culturing in hormone media can make meristem tip survive once time, and the result is better than two-time-cultivation.3、The meristem tip with two phyllomes(0.3-0.4mm in size)were found to suitable for virus-free seeding culture that were cut from aseptic seeding, which led to higher survival and virus-free ratio, up to 72.1%and 54.2%.

在培养基中添加椰汁、香蕉汁等有机添加物可以明显提高成苗率。3、采用二次剥离茎尖的方法，从无菌试管苗上切取带2个叶原基(大小0.3-0.4mm)茎尖分生组织培养，成苗率为72.1%，脱毒率为54.2%。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究，得出了优化的分离培养条件，即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后（各步间均用无菌水冲洗3~4次），研磨并稀释至10^(-3)，涂布于TSA培养基上，置28℃恒温条件下培养5-7d，可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。