次培养基

The highest enzyme producer alkalophilic Bacillus sp SHY8-1 among the inductive strains was mutagenized several times, by adding enzyme production repression aegents into screen medium, combined with natural seperation and pressure selection, finally a CMCase-hyperproductive mutant SHY8-1-25 (partitally resistant to catabolite) was obtained, with the yield of CMCase raised to 13-16u/ml, maximally reached 18-20u/ml, while its parent strain 0.654u/ml.

对诱导型产酶菌-嗜碱芽孢杆菌SHY8-1进行了多次诱变改良，采用添加产酶阻遏剂的平板作为筛子，结合反复的自然分离、压力选择，最终成功地获得了高产变异株SHY8-1-25(已部分解除分解代谢阻遏)，产酶量由0.654u/ml提高到13-16u/ml，最高达到18-20u/ml，讨论了诱变机理策略，考察了碳、氮源等对产酶的影响，对培养基和摇瓶培养条件进行了优化。

The main body of a book adopt the MRS culture medium flat to sift a lactobacillus , separate a purification by that 4 time of the above biography replaces it with reaching in a row,shape and rhabditiform the mirror is observed to hammer when checking up two kinds, the hammer shape is that the circle steeps a shape , become a pair or link up , assume strong point muchly catenoid existence; Long in being slender rhabditiform rhabditiform , strong arrangement of chain, large amount o'clock presents to send out the filiform congeries.

摘要本文采用MRS培养基平板筛选乳酸菌，经过连续4次以上的传代，以达到分离提纯，镜检时观察到链球状和杆状两种，链球状为圆泡状，多成对或串连，呈长链状存在；杆状为中长细杆状，链壮排列，大量时呈发丝状堆积。

The leafstalk and stem are facile induced to relaxed callis, and it could be the original of suspension culture by subculture many times. Its content of resveratol is influenced by the different parts of plant and culture condition.

葡萄叶柄和茎易被诱导产生松散的愈伤组织，且经多次继代可以用于建立悬浮培养体系；葡萄愈伤组织中的白藜芦醇含量随外植体及培养基组成的不同而有所差异。

The first division of protoplast occurred within 3 to 5 days in modified MS liquid media (1/10 strength macroelement) supplemented with 2 mg/L NAA+0.5 mg/L 6-BA and several organic compounds.

培养在1/10 MS附加2 mg/L NAA+0.5 mg/L 6-BA和多种有机成分培养基上的铁皮石斛无菌苗叶片原生质体，3~5 d后开始第一次分裂，30d后长成胚性细胞团。

Biological morphology and cell cycles were analyzed by morphometry and flow cytometry.

如此循环数次，直至A549细胞能在含有5.0nmol/L紫杉醇的培养基稳定生长。

A human promyelocytic leukemia cell mutant (HL-60-AR) with deficiency in HGPRT and resistance to 8-AG was established by treat〓nt of the HL-60 cells with MNNG and prescreened in 0.3% agar RPMI-1640 semisolid medium containing 8-AG. The mutant cells show the following characteristics:(1) resistant to cytotoxic action of 8-AG and growing vigorously in medium containing 20ug/ml 8-AG;(2) capable of forming 〓〓 colonies in soft agar medium in the presenee of 8-AG:(3) highly sensitive to HAT selective medium and fail to growing in HAT medium of form colonies in soft agar medium containing HAT;(4) completely deficinet in HGPRT activity; and (5) no radioactive silver grains over the mutant cells due to the absence of -hypoxanthine incorporation showed by radioautography.

一、用MNNG诱变和8-AG进行抗性筛选，成功地建立了一株抗高浓度8-AG和完全缺失HGPRT活性的人早幼粒白血病细胞突变株(HL-60-AR)，该细胞能抵抗8-AG的毒性作用，对HAT选择培养基十分敏感，检测不到HGHRT活性，不能参入〓H一次黄嘌呤。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

The advantages of the simple twice zymotechnics lie in the characters of the cultivation materials, the growth of mycelia, the control of plant diseases and insect pests, the output of Volvariella Volvacea and the production cost. All these indicate that simple twice zymotechnics is a relatively reasonable compost treatment in Volvariella Volvacea cultivation.

简易二次发酵法的栽培材料理化性状、草菇菌丝生长、病虫害控制和草菇的产量、生产成本等，都比自然堆沤发酵法和常规二次发酵法有较大的优势，表明简易二次发酵法是草菇栽培中较理想的一种培养基处理方法。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

If more than the upper one-third of the medium has acquired a pink color, the medium may be restored once by heating the containers in a water-bath or in free-flowing steam until the pink color disappears and by cooling quickly, taking care to prevent the introduction of nonsterile air into the container.

如果超过上部三分之一的培养基已经呈粉色，可以用以下方法恢复该培养基一次：在水浴锅中或者自由流动蒸气中加热该容器，直至粉色消失，并迅速放凉，须小心防止非无菌空气进入到容器中。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。