次培养

Temporarily catheterization pulls out inside 24 H ureter, if undertake uric education or acute retention of urine wait,adopt temporarily catheterization; leaves buy catheterization to be in clinical on common, ureter needs to leave above of buy 24 H, urethral mouth is daily want clean disinfection 2 times, daily change 1 times; keeps bag of collect make water or drainage bottle the person that buy catheter exceeds 7d, change every week ureter 1, with 0 · normally according to the circumstance of fluid of eduction make water 9% physiological saline undertake bladder is rinsed. 2 common problems patient of a few males is opposite circumstance of the psychogenic disorder when 1 catheterization of 2 · nurse catheterization produces psychogenic disorder, often be flushed, next abdomen and perineal ministry muscle are nervous, shadow fugleman make water goes on wheels, impact of urgenter to the illness patient psychogenic disorder is not big, ache and bladder are bouffant the mood that held them, catheterization process is more successful. 2 · 2 see the phenomenon is cracked below road junction of female patient make water occasionally 20 old clinical nurse in the job, the female cracks a circumstance to see 4 below urethral mouth.

临时导尿在24 h内拔除尿管，如进行尿培养或急性尿潴留等采取临时导尿；留置导尿在临床上常见，尿管需留置24 h以上，尿道口每日要清洁消毒2次，每日更换集尿袋或引流瓶1次；留置导尿管超过7d者，每周更换尿管1次，根据排出尿液的情况通常以0·9%生理盐水进行膀胱冲洗。2常见新问题2·1导尿时心理障碍情况一些男性患者对护士导尿产生心理障碍，往往面红耳赤，下腹部及会阴部肌肉紧张，影响导尿顺利进行，对于病情较急的患者心理障碍影响不大，疼痛和膀胱鼓胀占据了他们的情绪，导尿过程较顺利。2·2偶见女性患者尿道口下裂现象20多年的临床护理工作中，女性尿道口下裂情况见4例。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Some key techniques related to the close and continuous process were investigated by the application of H9N2 avian influenza virus with Vero cells, such as the susceptibility of cell to influenza virus, virus production with cell microcarrier culture method, cell bead-to-bead transfer, virus production through bead-to-bead transfer, cell culture and virus production with serum free medium, metabolism analysis, and repetitiously intermittent bead-to-bead transfer of cell for virus production to simulate the close and continuous process.

通过使用Vero细胞增殖禽流感病毒H9N2，本文针对封闭连续工艺过程的一些关键技术开展研究，包括细胞对流感病毒敏感性分析、细胞微载体培养生产病毒工艺、细胞珠到珠转移、转移后细胞对病毒增殖敏感性验证、细胞无血清培养生产流感病毒、代谢分析、可模拟连续操作的多次间歇式珠到珠转移培养细胞生产流感病毒等方面。

The four-week twice immunized hamsters acquired anti-MP in-fection capacity in local respiratory tract,as a result that swab culuturepositise was belove that of unimmunization group,and that only onehumster's lavage fluid culture was positive in immunization group.How-ever,unimmunization group positive rate of swabs and lavage fluid cul-ture was very high.This hinted that local respiratory tract's anti-MPinfection capacity was weak at the primary infection and the ability de-veloped gradually.4.The sIgA change in broncheal-lung lavage fluid.

而未免疫组咽拭子和支气管肺泡灌洗液MP分离培养的阳性均很高，说明在初次感染时，呼吸道局部防御清除MP的作用差，但随着时间的推移，其灌洗液培养的周次阳性率有下降趋势，MP培养的滴度亦随之降低，说明在感染以后随着时间的推移，呼吸道局部逐渐产生了防御、清除MP感染的作用。

Methods: NG108-15 cells were cultured in vitro at 37℃in a humidified atmospherecontaining 5％CO_2. Aβ_(25～35) fragment was dissolved with deionized distilledwater and aged for 4 days at 37℃. The cells were exposured to 5μmol/LAβ_(25～35) for 24h to establish the cell model. Twelve rabbits were divided intotwo groups at random, and garaged with MWD or physiological saline controlrespectively for 7 days to collect two kinds of cerebrospinal fluid, theyare CSF from rabbits treated with MWD and CSF from rabbits treated withphysiological saline.

NG108-15细胞在37℃、体积分数为5％的CO_2培养箱中培养，Aβ_(25～35)溶解于双蒸水中，置于37℃培养箱中老化4d，使其寡聚，毒性增强，细胞被置于浓度为5μmol／L的Aβ_(25～35)中作用24h，建立起AD细胞模型。12只清洁级大耳白家兔购于华中科技大学同济医学院动物中心，将其随机分成两组，分别给与等量的加味温胆汤和生理盐水灌胃7d，末次给药后1h内用水合氯醛将给药组白兔麻醉，1.2ml／kg，无菌条件下用7号注射针头从枕骨大孔处垂直进针，抽取清亮含药脑脊液。

After calculating the concentration by counting board, cell suspension was cultured on the coverslips coated poly-L-lysine at 1×109/L, then the coverslips were paved in the bottom of 35 mm culture dish for 3 weeks.

经12 h+ 24 h两次差速培养后，吸出细胞上悬液，计数板计算浓度后，按1×109 L-1浓度重新种植于载有多聚赖氨酸包被盖玻片的35 mm培养皿中继续培养3周。

This gies me the opportunity to share some of my personal experience with you all and also proidse me the opportunity to contribute, in a way,to the country and people who trained me in the first place to be an ophthalmologist.

这给我一次和各位分享我个人经验的机会，在某种意义上来说，也给了我一次对培养我做眼科医生的国家和人民做一次贡献的机会。

Then, they were transferred into the mycelium culture fluid which includes 30% sucrose and 1% or 1.5% glycin under the condition of 20℃, 150 rpm for 48 h. In order to obtain protoplasts of mass and high quality, the strain F46 protoplasts given after combination use of the 10 mg·mL-1 lysozyme and 10 mg·mL-1 snailase at 34℃for 3 h; and the SC1 was treated by 10 mg·mL-1 lysozyme at 34℃for 2.5 h.

确定了双亲原生质体形成与再生的最佳条件：菌株F46、SC1采用二次菌丝培养，即在蔗糖含量20%菌丝培养液里，25℃条件下150 rpm培养48 h；然后按15%的接种量转接到蔗糖含量30%，甘氨酸含量分别为1%、1.5%的菌丝培养液中，同样条件下，继续培养48 h；在34℃条件下，菌株F46用等量混合的蜗牛酶和溶菌酶20 mg·mL-1，菌株SC1用10 mg·mL-1溶菌酶，分别酶解3 h、2.5 h，不仅有大量的原生质体形成，且再生率也高。

That competitive sports insists that "to strike ice and snow plate , to sing ice and snow play " for development strategy,creates good achievement on surpassing field at home and abroad.

竞技体育坚持以&打冰雪牌、唱冰雪戏&为发展战略，在国内外赛场上创造佳绩。20 年来，共获得奖牌2300 余枚，有1 人2 次打破1 项世界青少年速滑纪录、7 人11 次打破9 项全国纪录，9 人9 次打破6 项全国青少年纪录、30 人41次打破31 项省纪录、51 人79 次打破57 项省青少年纪录、99 人195 次打破176 项市记录、先后为省和国家培养出了全国、亚洲、世界冠军焦志敏、陈静、郭伟茹、杨光、曹桂凤、冯庆波、刘广彬、卢亚军、于凤桐、任慧、许文龙、张爽等一批优秀体育人才，他们为祖国和家乡争得了荣誉，使林城伊春走向全国、走向世界。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究，得出了优化的分离培养条件，即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后（各步间均用无菌水冲洗3~4次），研磨并稀释至10^(-3)，涂布于TSA培养基上，置28℃恒温条件下培养5-7d，可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。