次培养

In retrospect, it is known that along with the fledging of the third science and technology revolution and the rapid progress of modern technology, universities and colleges dedicated to technical education sprang up like mushrooms in most of developed countries and regions since 1960s.

回溯历史，随着第三次科技革命的出现以及现代技术的快速发展，20世纪60年代以来，在世界上部分发达国家和地区，纷纷出现了一批以举办本科技术教育为主体的高等院校，这些院校特有的办学模式及其高质量的人才培养，在高等教育大众化的背景下，不但满足了社会对技术型人才的需求，也促进了高等教育多元化的发展。

Since 1978, to meet the requirements of afforestation, some 50 sapling farms have been set up in various places, introducing, naturalizing and cultivating scores of tree breeds suitable for Tibet.

这些科研成果，为西藏在经济建设和发展中更好地利用自然资源，不断改善人类生存环境提供了科学依据。1977年，国家农林部组织了对西藏全区的第一次全面森林资源清查。1978年起，为适应造林绿化的需要，各地先后建立苗圃近50个，引进、驯化、培养了数十种适宜西藏生长的造林绿化树种。

These cells can be cloned repeatedly and maintained in long-term culture. Our studies are the first to show NIPs derived from human fetal pancreas, which may have significant implications for future applications in stem cell therapy of diabetes.

这些细胞可以被反复克隆并长期培养，我们的研究第一次揭示了胎儿胰腺来源地NIPs，在糖尿病干细胞疗法的应用方面可能具有一定的价值。

Temperature came downtowns 38 c, three times blood cultures were negative. Sept 6 Ventricular tachycardia recurred, it was controlled by bolus and continuous intravenous infusion of Lidocaine, but the BP and PaO2 could not be maintained, pulmonary edema supervened, so IABP was inserted again.

体温控制在38℃以下。3次血培养均为阴性。9月6日又出现室性心动过速，以利多卡因冲击量和维持景治疗能控制心律紊乱，但是不能维持血压和Pa0：，同时出现肺水肿，因此再度置入主动脉弓内球囊反搏。

For instance, the Jesuits were responsible for creating a Latinized alphabet and a grammar book that became the basis for the modern form of Vietnamese writing, forming an "Institution of Catechists" which evolved into the first native clergy, and contributed to the formation of Vietnamese clergy after they returned to Vietnam the second time.

例如，耶稣会士为越南语设计的拉丁化字母及编写的语法书，奠定了现代越南书面语的基础，此外还成立了一个&教义问答师所&。它培养了第一批本地神父，在耶稣会第二次返回越南后，为越南神职队伍的形成做出了贡献。

Small-scale fermentation (60ml culture medium in a 500 ml Sakaguchi flask) was carried out followed by antioxidant screening using lipid auto-oxidation and TBARS (thiobarbituric acid reactive substances) as the detection methods.

针对所分离的菌株，我们采用往复式振荡发酵方式进行菌种批次培养以生产二次代谢物，并配合抗氧化活性检测的方法进行抗氧化物质生产菌的筛选。

Thetransformed fibroblasts developed well and kept good reproduction ability till the eighty-fifth generation.

经转化的细胞在体外培养传代85次，细胞生长状态良好，仍然保持旺盛的增殖能力。

In addition, activation and inactivation of p34cdc2are induced by Ca2+and prevented by Ca2+ chelators in permeabilized cells and cell - free systems.

细胞用胰蛋白酶N.2％）消化后，在 PBS中洗2次，1200 x g离心 10分钟去上清，加人 70％的冷乙醇，于一20t 30分钟。去除乙醇后，细胞中加人含有 RNase AN.lin牙IIl）的 PBS中放在 37 t培养箱 30分钟。

RPMI 1640 medium to induce hyphae at 37℃ for seven days was thought to be the favorable condition for C.

RPMI 1640培养基（P＝7.5），37aC传代培养7天（转种 12次），是获得菌丝相白念珠菌的理想条件； 3。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原，免疫BALB/c小鼠，按常规方法进行融合，间接ELISA方法进行筛选，采用有限稀释法，经过三次克隆，最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5)，分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类，三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。