次培养

Quadratic regressive factorial experiment was used to optimize concentration of 6-BA and NAA in MS medium for shoot propagation from the top buds and stem segments of Russelia equisetiformis.

以爆仗竹顶芽与带腋芽的茎段为外植体，采用二次回归正交设计方法研究了爆仗竹组织培养快速繁殖中不定芽增殖系数与继代培养基中6BA和NAA组合及其浓度配比之间的关系。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色，鉴定成肌细胞标志蛋白——结蛋白的表达，弃去desmin阴性的细胞克隆，继续培养desmin阳性的细胞克隆，隔天换液1次，7 d进行酶消化传代，获得大量扩增的细胞，并可冻存复苏，用于实验。

From the first, her Grand-mama had chosen Ambreene to be her friend and sorcerous heir, and shaped her into the role coldly and caleulatingly.

第一次，她的奶奶选择艾布瑞妮作为她的朋友和魔法继承人，并按这个目标培养她，冷酷而深谋远虑。

Making stratiform evaluation on them is beneficial to swap them to participate in physical training ac- tively and consciously,and to cultivate their lifelong physical awareness.

分层次评价有利于调动学生参与锻炼的积极性、主动性，培养他们终身体育意识。

Whereas, the isolation rate of this three Vibrio spp. from the culture method was low toxigenic V.

但无论如何，传统的培养方法於今次的研究当中只作为参考之用，因其检测的能力是较以分子方法检测为低。

According to the above results, feather, tryptone, yeast extract as well as incubation time and temperature were selected for further studies by RSM. Data obtained showed the maximal biomass production and keratinase activity occurred at above 96 h.

根据上述结果选择feather、tryptone、yeast extract外加两个物理因子进行第一次的RSM分析，结果发现欲得到较高的角蛋白酶活性与菌体产量，其培养时间需超过四天以上。

The twenty-eight bacteria are cultivated and put back into waste water,the average unoiling rate can achieve to 68%.

通过 3次采自乌鲁木齐市鸿春园饭店不同时期排放的污水，经培养筛选出的 2 8株具有较强除油能力的菌种回接污水后，其平均除油率达 68%，其优选菌种回接污水 2 4h后的除油率达 90 %，而同批污水自然存放 1 0d后的除油率仅为 2 9%。

It is unsensible to buy ESCs from other labrotaries, because of the pluripotency of ESCs would be affected by passage number and freeze thawing. So we need to built steadily mouse embryonic stem cells-derived and tested system.

因为ES细胞的多能性会受到多次传代以及冻融的影响，因此需要在本实验室条件下建立起ES细胞稳定的分离培养和鉴定体系。

But on one occasion, he had to show a visitor what he had been researching, and so he retrieved some of the unsubmerged dishes that he would have otherwise discarded, when he then noticed a zone around an invading fungus where the bacteria could not seem to grow.

但在一次偶然的机会中，他要给一位来访者展示他的研究，所以他拿了几个先前丢掉的培养皿。这时，他发现在侵入的真菌周围有一片区域的细菌好像停止了生长。

There was also no morphological difference between the two explants.

皮质骨两次植块培养的细胞形态学上无明显差异。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。