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The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸1-氨基环丙烷-1-羟酸氧化酶基因cDNA片段,将片段序列在NCBI网站上进行同源性搜索,显示的皆为不同植物的ACC氧化酶基因,因而认为所克隆的片段就是酸ACC氧化酶基因;并运用DNAStar5.0软件进行序列分析,推导的氨基酸序列为278个残基;具有所有植物ACC氧化酶基因共有的保守区域;与多种植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上。

METHODS The contents of synephrine,neohesperidin,hesperidin, naringin and narirutin in the raw materials and the extracts of Citrus aurantium were determined by reversed-phase high performance liquid chromatography with an Inertsil ODS-3(4.6 mm×250 mm,5 μm) column, respectively.

方法采用高效液相色谱法,以InertsilODS-3(4.6mm×250mm,5μm)色谱柱分别对枳实及其提取物中辛弗林生物碱类成分和新皮苷、皮苷、柚皮苷、芸香柚皮苷黄酮类成分进行测定。

Moisturizer:Water,propylene glycol,saccharide isomerate,chondrus crispus,peg-40 hydrogenated castor oil,imidazolidinyl urea,methylparaben,sodium hyaluronate,pentadecalactone,citrus aurantium dulcisoil,lactic acid,iris germanica root extract,sodium hydroxide,citrus aurantium amara oil,yellow 6(CI 15985),limonene,linalool

保湿精华:水,丙二醇,天然异构寡糖,卡拉胶,PEG-40氢化蓖麻油,尿素醛,羟苯甲酯,透明质酸钠,环十五内酯,甜油,乳酸,鸢尾花根萃取,氢氧化钠,苦油,黄 6(CI 15985),柠檬精油,芳樟醇

Water,butylene glycol,biosaccharide gum-1,ppg-26-buteth-26,panthenol,peg-40 hydrogenated caster oil,hydroxyethylcellulose,imidazolidinyl urea,saccharide isomerate,benzophenone-4,phenoxyethanol,triethanolamine,citrus aurantium dulcisoil,pentadecalactone,methylparaben,sodium hyaluronate,citric acid,butylparaben,ethylparaben,citrus aurantium amaraoil,propylparaben,methylchloroisothiazolinone,methylisothiazolinone,red 4,yellow 5,red 33,limonene,linalool

水,丁二醇,多醣物质,PPG-26-丁醇聚醚-26,维生素B5,PEG-40氢化蓖麻油,羟乙基纤维素,尿素醛,天然异构寡糖,二苯甲酮- 4,苯氧乙醇,三乙醇胺,甜油,环十五内酯,羟苯甲酯,透明质酸钠,柠檬酸,羟苯丁酯,羟苯乙酯,苦油,羟苯丙酯,甲基氯异塞唑晽酮,甲基异噻唑啉酮,红4,黄5,红33,柠檬精油,芳樟醇

Water,cetearyl alcohol,butylene glycol,ceteth-20,glycolic acid,cyclomethicone,citric acid,sodium hydroxide,xanthan gum,lactic acid,phenoxyethanol,sodium citrate,magnesium aluminium silicate,citrus aurantium dulcisoil,disodium edta,pentadecalactone,methylparaben,iris germanica root extract,butylparaben,ethylparaben,propylparaben,silica,chlorhexidine digluconate.citrus aurantium amaraoil,limonene,linalool

水, 棕榈醇,丁二醇,鲸蜡醇聚醚 20,甘醇酸,环甲硅脂,柠檬酸,氢氧化钠,山羊胶,乳酸,苯氧乙醇,柠檬酸钠,硅酸镁铝,甜油,乙酸乙二胺二钠,环十五内酯,羟苯甲酯,鸢尾花根萃取,羟苯丁酯,羟苯乙酯,羟苯丙酯,二氧化硅,洗必泰葡萄糖酸盐,苦油,柠檬精油,芳樟醇

Water,butylene glycol,glycerin,glyceryl polymethacrylate,triethanolamine,carbomer,ppg-26-bueth-26,chondrus crispus,peg-40 hydrogenated castor oil,phenoxyethanol,imidazolidinyl urea,propylene glycol,benzophenone-4,retinyl palmitate,citrus aurantiudulcisoil,phospholipids,methylparaben,tocopheryl acetate,c12-23 alkyl lactate,disodium EDTA,centella asiatica extract,iris germanica root extract,pentadecalactone,aloe barbadensis leaf juice,mimosa tenuiflora bark extract,sodium hyaluronate,menthol,biosaccharide gum-1,citrus aurantium amara oil,propylparaben,methylchloroisothiazolinone,sodium benzoate,potassium sorbate,citric acid,calcium sorbate,methylisothiazolinone,butylparaben,ethylparaben,yellow 5(CI 19140),limonene,linalool

水,丁二醇,甘油,甘油聚甲基丙烯酸酯,三乙醇胺,卡波姆,PPG-26-丁醇聚醚-26,鹿角菜胶,PEG-40 氢化蓖麻油,苯氧乙醇,尿素醛,丙二醇,二苯酮-4,维他命A酯,甜油,磷脂质,羟苯甲酯,醋酸盐维他命E,C12-13 烷醇乳酸酯,乙二胺四乙基二钠,雷公根萃取,鸢尾花根萃取,环十五内酯,吉拉索芦荟叶汁,含羞草萃取,透明质酸钠,薄荷脑,多醣物质,苦油,羟苯丙酯,甲基氯异塞唑晽酮,苯甲酸钠,山梨酸钾,柠檬酸,山梨酸钙,甲基异噻唑啉酮,羟苯丁酯,羟苯乙酯,柠檬黄,柠檬精油,沈香醇水:几乎所有护肤品成分第一位都是水。丁二醇:保湿剂及溶剂,质地温和

Water,c12-20 acid peg-8 ester,octyldodecanol,cetearyl isononanoate,butylene glycol,biosaccaride gum-1,peg-40 hydrogenated caster oil,ethylhexyl menthoxycinnamate,phenoxyethanol,potassium cetyl phosphate,macadamia ternifolia seed oil,dimethicone,carbomer,propylene glycol,glyceryl stearate,cocoglycerides,butyrospermum parkii,c12-13 alkyl lactate,methylparaben,benzophenone-3,pentadecalactone,sorbic acid,citrus aurantium dulcis oil,sodium hydroxyde,tocopheryl acetate,disodium edta,iris germanica root extract,retinyl palmitate,equisetum arvense extract,butylparaben,peg-8,propylparaben,ethylparaben,sodium hyaluronate,chlorhexidine digluconate,citrus aurantium amaraoil,tocopherol,ascorbyl palmitate,citric acid,ascorbic acid,limonene,linalool

水,c12-20 acid peg-8 ester,辛基十二烷醇,鲸蜡硬脂醇异壬酸酯,丁烯二醇,多醣物质,PEG-40氢化蓖麻油,4-甲氧基肉桂酸-2-乙基己基酯,苯氧乙醇,十六烷基磷酸钾,澳洲胡桃子油,地美司康,卡波姆,丙烯乙二醇,甘油硬脂酸,椰油脂酸甘油酯类,牛油果,c12-13烷醇乳酸酯,对羟基苯甲酸甲酯,二苯甲酮-3 ,环十五内酯,山梨酸,甜油,氢氧化钠,维生素E醋酸酯,乙二胺四乙酸二钠,鸢尾花萃取,维他命A酯,问荆萃取,对羟基苯甲酸丁酯,聚乙二醇- 8,羟苯丙酯,羟苯乙酯,透明质酸钠,洗必泰葡萄糖酸盐,苦油,维生素E ,维生素C棕榈酸酯,柠檬酸,抗坏血酸,柠檬精油,沉香醇水:几乎所有护肤品成分第一位都是水。辛基十二烷醇:界面活性剂、乳化剂、稠化剂,有一定刺激性

Water,propylene glycol,butylene glycol,glyceryl polymethacrylate,ascorbic acid,peg-7 glyceryl cocoate,biosaccharide gum-1,peg-40 hydrogenated castor oil,ppg-26-buteth-26,phenoxyethanol,hydroxyethylcellulose,xanthan gum,methylparaben,pentadecalactone,citrus aurantium dulcisoil,disodium edta,sorbic acid,tocopheryl acetate,butylparaben,retinyl palmitate,iris germanica root extract,sodium hyaluronate,ethylparaben,propylparaben,citrus aurantium amara oil,limonene,linalool

水,丙二醇,丁二醇,甘油聚甲基丙烯酸酯,维生素C衍生物,PEG-7 椰子油酸单甘酯,多醣物质,PEG-40氢化蓖麻油,PPG-26-丁醇聚醚-26,苯氧乙醇,羟乙基纤维素,山羊胶,羟苯甲酯,环十五内酯,甜油,乙酸乙二胺二钠,山梨酸,醋酸盐维他命E,羟苯丁酯,维他命A酯,鸢尾花根萃取,透明质酸钠,羟苯乙酯,羟苯丙酯,苦油,柠檬精油,芳樟醇

The oxidation of methyl linoleate was inhibited by β-carotene, lutein and bixin in a dose-dependent manner. Lutein and zeaxanthin suppressed the hydroperoxide formation more efficiently than β-carotene and bixin at 41.7×10-6mol/L. Lutein inhibited the oxidation of methyl linoleate as efficiently as zeaxanthin.

表明β-胡萝卜素,叶黄素及胭脂树都按依赖于剂量的方式抑制亚油酸甲酯氢过氧化物的形成。41.7×10 -6mol/L的叶黄素及玉米黄质的抑制活性相近,高于β-胡萝卜素与胭脂树的活性,后两者活性近似。

At the end of the corridor was the dressing room belonging to Clarisse and Simonne.

黄色的天花板像被火烧焦似的,在黄的云雾中,一盏灯笼点燃着。

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I didn't watch TV last night, because it .

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