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Objective To investigate the differentiation of bone marrow-derived mesenchymal stem cells into neurons and transplantation of the stem cells to repair rat hemisection spinal cord injury.

目的 初步探讨骨髓间充质干细胞诱导为神经细胞,及其移植对大鼠脊髓半断损伤神经功能恢复和运动的影响。

Methods 20 rats were divided into control group, L-NNA group, OECs group, OECs+L-NNA group after spinal cord hemisection.

将20只大鼠行脊髓半断术后分为对照组、OECs组、L-NNA组和OECs+L-NNA组。

The rat model of hemisection spinal cord injury was prepared and BrdU was locally injected to label the induced neurons.

制备大鼠半断脊髓损伤模型,脊髓损伤局部注射BrdU标记诱导后的神经细胞。

Keywords Spinal cord hemisection injury;Embryonic stem cells;Transplantation

脊髓半断损伤;胚胎干细胞;移植

Objective To observe the effects of microencapsulated rabbit sciatic nerve tissue transplantation on substance P expression in dorsal root ganglia after spinal cord hemisection injury.

目的 观察微囊化兔坐骨神经组织细胞移植对脊髓半断后脊神经节P物质表达的变化。

Methods ESCs were collected,induced and marked with green fluorescent protein before transplantation.6 healthy adult cats were randomly divided into 2 groups,normal transplanted group and spinal cord hemisection transplanted group(n=3/each group).

结果绿色荧光蛋白标记的胚胎干细胞的阳性率为38%,半断移植组未见到荧光细胞,正常移植组可看到荧光细胞并迁移一段距离。

Materials and Methods: Forty Sprague-Dawley rats were randomly divided into normal group, control operative group and operative group which includes 6 subgroups (1,3,7,14,21,28 days). The spinal cord hemisection injury model was made by cutting the posterior right side spinal cord. Function changes in the right posterior limb were evaluated by BBB method. The general morphology changes were observed by HE stain.

材料与方法:40只成年SD大鼠随机分为正常组,假手术组和手术组,手术组又分成1,3,7,14,21,28天组,选取制作脊髓右侧半断损伤模型,BBB法进行右下肢功能评分,HE染色观察损伤脊髓不同时段的大体形态学变化,原位杂交法观察slit-2 mRNA的表达变化,计算阳性细胞灰度值,进行统计学分析。

Objective To investigate the survival, migration and function of mouse embryonic stem cells implanted into spinal cord of cats at acute stage following spinal cord hemisection injury.

正常组和半断组同时移植带有绿色荧光小鼠胚胎干细胞,7 d后灌注取材并行脊髓组织切片后,在倒置荧光显微镜下观察干细胞存活及迁移情况和对行为功能的影响。

Objective To observe the effects of Schwann cellsand MK801 used respectively or jointly on the neuronal survival and expression of nitric oxide synthasein Clarke's nucleusafter spinal cord hemisection of rats.

目的 观察单独或联合应用施万细胞和MK80 1对大鼠脊髓半切后受损伤的背核神经元存活及其表达NOS的影响。

MSCs induced by spinal cord homogenate supernatant can be transplanted into hemisection spinal cord injury and improve the motor function of the injuries lesions.

MSCs经脊髓匀浆上清诱导后移植治疗大鼠半断脊髓损伤可使运动功能得到改善。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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