模板

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

After tweaking to make it just right, it was put on a floppy disc and inserted into a computer-controlled mill to cut a template.

经过调整，使之恰到好处，这是把软盘和光盘插入到计算机控制的工厂削减一个模板。

Each flyweight type, that is, each distinct instantiation of the class template flyweight , is associated with exactly one factory object.

每个flyweight类型，就是说，每个 flyweight 类模板的不同实例，只会有一个相关的工厂对象。

Then, gently hold the template in place on your fondant and use a toothpick to trace the heart shape.

然后，轻轻地按住模板放在您翻糖和使用牙签追踪心形。

The diameter of the physiological foramen was measured with the software length measuring mode.

使用软件长度测量模板测量生理性根尖孔的直径，直径小于0.10mm的孔为副孔。

Reagents such as glycerol,DMSO,formamide are usually used to improve the efficiency of PCR for the template with high G+C PCR.

PCR已经成为现代生命科学研究的一项最基本的技术，但是当模板的G+C含量比较高时，PCR常常出现扩增效率低甚至扩增不出来的情况。

That example contained logic in the format of a Python object and new pages in the format of page templates.

这个例子包括Python形式的对象和一个页面模板形式的新页面。

To symplify the rather complex basic geometrie and to facilitate its construction, each arc is formed by an identical element of formwork.

为了symplify的相当复杂的基本几何并促进其建设，每个弧是由相同的内容模板。

To simplify the rather complex basic geometrie and to facilitate its construction, each arc is formed by an identical element of formwork.

为了简化这个复杂的几何形体同时减小建造难度，每个拱形都由相同的模板搭建而成。

In the construction design and manufacture of steel formwork has a wealth of experience.

在建筑钢模板设计和制造方面有着丰富的经验。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。