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CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板,根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物,利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段,将其连接到pMD18-T载体,转化大肠杆菌后筛选阳性克隆并进行序列测定,测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板,根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物,利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段,将其连接到pMD18-T载体,转化大肠杆菌后筛选阳性克隆并进行序列测定,测序得到的1 335 bp的片断编码444个氨基酸残基。

In the form and supporting structure of perform hole for the shield machine passing the station not only the vertical gravity of the new concrete, but also the lateral pressure and the buoyance are suppored.

盾构过站预留孔洞的模板和支顶体系,除了受到新浇筑混凝土的重力之外,还要受到新浇筑混凝土的侧压力和上浮力。

Complex--valued kernel structure element frequency filtering morphological centroid scale--space image parallel processing system has been proposed and established, in which the complex--valued kernel structure element frequency filtering CGH was designed and made by the way of Burckhardt which could improve recording accuracy and reduce quantity noise.

提出并构造了复值核结构元频域滤波形态学矩心尺度--空间图象处理系统,其中采用编码精度高和量化噪声小的Burckhardt方法设计和制作了复值核结构元频域滤波器CGH模板,并对系统处理图象的面积、系统对CGH复位精度的要求进行了分析。

In addition, it offers drop shadow effects, button text, background images and customizable lightning angles.The program does not require any graphical expertise, everything is point and click. The results canbe exported to JPG, GIF or PNG file.

你可以从范围广泛的预定义形状的网页按钮当中进行选择,或者通过创建来定制你自己的按钮或者导入你自己的模板,并且可以为它们应用各种不同的定制功能和效果。

"It is widely used both in cabinetmaking (for chests, dressers, wardrobes, and tables) and in house construction (for walls, ceilings , subfloors, doors, and in forms for casting concrete)."

它被广泛应用于家具板材和房屋建造(墙体、天花板、底层地板、门板和水泥模板)。

"It is widely used both in cabinetmaking (for chests, dresser s, wardrobes, and tables) and in house construction (for walls, ceilings, subfloors, doors, and in forms for casting concrete)."

它被广泛应用于家具板材和房屋建造(墙体、天花板、底层地板、门板和水泥模板)。

"It is widely used both in cabinetmaking (for chest s, dressers, wardrobes, and tables) and in house construction (for walls, ceilings, subfloors, doors, and in forms for casting concrete)."

它被广泛应用于家具板材和房屋建造(墙体、天花板、底层地板、门板和水泥模板)。

"It is widely used both in cabinetmaking (for chests, dressers, wardrobes, and tables) and in house construction (for walls, ceiling s, subfloors, doors, and in forms for casting concrete)."

它被广泛应用于家具板材和房屋建造(墙体、天花板、底层地板、门板和水泥模板)。

"It is widely used both in cabinetmaking (for chests, dressers, wardrobes, and tables) and in house construction (for walls, ceilings, subfloors, doors, and in forms for cast ing concrete)."

它被广泛应用于家具板材和房屋建造(墙体、天花板、底层地板、门板和水泥模板)。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

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