槲皮苷

Objective To develop an RP-HPLC method for the simultaneous determination of the contents of protocatechuic acid, rutin, hyperoside and quercitrin in Bidens parviflora Willd..

目的 建立同时测定小花鬼针草中原儿茶酸、芦丁、金丝桃苷和槲皮苷含量的方法。

Results The linear ranges of protocatechuic acid, rutin, hyperoside and quercitrin were 40.2-804.0, 24.8-496.0, 25.2-504.0, 20.4-408.0 mgL^(-1)(r≥0.999 5, n=6), respectively. The average recoveries were 100.2%, 99.3%, 99.5% and 98.3%(RSD.9%, n=6), respectively.

结果 原儿茶酸、芦丁、金丝桃苷、槲皮苷质量浓度分别在40.2~804.0、24.8~496.0、25.2~504.0、20.4~408.0 mgL^(-1)内与峰面积呈良好线性关系(r≥0.999 5， n=6)，方法平均回收率分别为100.2%、99.3%、99.5%、98.3%(RSD≤2.9%， n=6)。

Results: Seven compounds were isolated from the ethanol extract of the fruits and identified as oleanolic acid, luteolin-7-O-β-D-glucoside, quercitrin,β-sitosterol-3-O-β-D-glucoside, p-hydroxyphenethyl-β-D-glucoside, D-mannitol and hexitol.

结果：从女贞子的乙醇提取物中分离得到7个单体化合物，分别鉴定为齐墩果酸、木犀草素－7-O-β-D-葡萄糖苷、槲皮苷、β-胡萝卜苷、对羟基苯乙醇－β-D-葡萄糖苷、甘露醇及己六醇。

There were different kinds of apple polyphenolics with different varieties of Gala and Golden. As it is known, the anthocyanidin is only in Gala Skin but not in Golden skin. It is found in this study that there has quercetin in Golden pulp but not be detected in Gala pulp.

嘎拉和金冠中多酚化合物种类不同，已知嘎拉果皮中含有花青素-山越橘苷，而金冠中没有；本实验发现金冠果肉中含有槲皮苷，而嘎拉果肉中没有检测到。

Quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E wereisolated from the flower of Acanthopanax senticosus. Searching bioactive natural compound and establishing quality evaluation standard arefocused on this experiment. Using cold immersing of 70% methanol to extract from flower ofAcanthopanax senticosus and integrating macroporous resin, silica gel column chromatography,sephadex gel to abstract. We obtained five monomer compounds (compoundⅠ-compoundⅤ),quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E. That five compoundswere determined by HPLC in eight parts of Acanthopanax senticosus (flower, root, leaf fruit,shoot, new shoot, phloem of shoot, skin of shoot), which provide science bases for establishingquality evaluation system and settle infarctate foundation.

本文以从刺五加花蕾中寻找具有重要生物活性的天然化合物和建立质量评价标准为主要目的，对其化学成分进行研究，采用70％甲醇冷浸的方法对刺五加花蕾中的成分进行提取，运用大孔树脂吸附色谱法、硅胶柱层析法以及葡聚糖凝胶柱层析相结合的方法进行分离，得到5种单体化合物，分别为槲皮素、槲皮素鼠李糖苷、金丝桃苷、紫丁香苷和刺五加苷E；利用高效液相色谱法对这5种化合物在刺五加花蕾中的含量进行了测定；考察了8个部位(花蕾、根、叶、果、茎干、新枝、茎韧皮部、茎皮)中这5种化合物的含量分布情况，并作一比较，为今后建立刺五加质量评价体系提供了科学依据，奠定了坚实的基础。

Objective to establish a method for simultaneous determination of rutin, hyperin, isoquercitrin, 4,5-O-dicaffeoyl quinic acid and isorhamnetin-3-O-glucoside in Herba Artemisiae Capillariae.

目的 建立同时测定茵陈中芦丁、金丝桃苷、异槲皮苷、4，5-O-二咖啡酰奎宁酸、异鼠李素－3-O-葡萄糖苷含量的方法，为茵陈药材的质量控制提供依据。

Results The linear response range was 0.9420-18.84mg L^(-1)(r=0.9995), 1.190-23.79 mg L^(-1)(r=0.9995), 1.107-22.14 mgL^(-1)(r=0.9994), 56.78-1.136×10^3 mgL^(-1)(r=0.9990) and 0.6219-12.44 mgL^(-1)(r=0.9998), respectively (n=5). The average recoveries (n=9) of rutin, hyperin, isoquercitrin, 4, 5-O-dicaffeoyl quinic acid and isorhamnetin-3-O-glucoside were 98.1%, 100.7%, 98.4%, 100.2% and 101.7%, respectively.

结果 芦丁、金丝桃苷、异槲皮苷、4，5-O-二咖啡酰奎宁酸、异鼠李素－3-O-葡萄糖苷质量浓度分别在0.942~18.84mgL^(-1)(r=0.9995)、1.190~23.79mgL^(-1)(r=0.9995)、1.107~22.14mgL^(-1)(r=0.9994)、56.78~1.136×10^3 mgL^(-1)(r=0.9990)、0.6219~12.44mgL^(-1)(r=0.9998)内与峰面积呈良好的线性关系(n=5)；方法回收率(n=9)分别为98.1%、100.7%、98.4%、100.2%、101.7%。

Results showed a close relationship existed among the three flavonoid compounds with kaempferol content greater than quercetin and isoquercetin.

结果表明：青钱柳叶中3种黄酮类化合物质量分数紧密相关，其中山奈酚质量分数高于槲皮素和异槲皮苷。

Assaying results indicate that in that eight parts of Acanthopanax senticosus content ofquercetin is highest in leaf,0.03818%, content of quercetin rhamnoside is highest in flower,4.43115%, content of hyperin is highest in flower,2.41605%, content of syringoside is highest inskin of shoot,1.18959%, content of eleutheroside E is highest in root,

含量测定结果表明：在刺五加花蕾、根、叶、果、茎干、新枝、茎韧皮部、茎皮这八个部位中，槲皮素叶中含量最高，为0.03818％；槲皮素鼠李糖苷花蕾中含量最高，为4.43115％；金丝桃苷花蕾中含量最高，为2.41605％；紫丁香苷茎皮中含量最高，为1.18959％；刺五加苷E根中含量最高，为0.18802％。

The extraction technology of quercitr in urtica was studied with ethanol as extractor.

对荨麻中槲皮苷的乙醇提取工艺进行了研究。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。