槲

The results of HPLC tests indicated that Bt toxin in Bt transgenic cotton had not obviously affected the contents of flavonoids (mainly rutin and isoquercitrin and a few quercetin ),contradictively ,the result of combined dosage test in vitro showed that that rutin had significantly enhanced the suppression of Bt-ICP on the cotton bollworm larvae., in addition to the

采用高效液相色谱法测定转Bt基因棉花主要抗虫黄酮类化合物的的变化，结果表明外源Bt杀虫蛋白表达不会对棉花自身原有的主要抗虫黄酮类物质（包括芸香苷、异槲皮苷和含量较少的槲皮素）产生不利的影响，因此可以将Bt杀虫蛋白基因转入抗虫黄酮类物质中国农业科学院博士学位论文含量高的棉花品系。

Quercetin is one of the effective components contained in sophora japonica flower bud.3-hydroxy 4-carbonyl,4-carbonyl 5-hydroxy,and 3′,4′-dihydroxy groups of quercetin could be become chelating position with metal.

槲皮素（3，5，7，3′，4′－五羟基黄酮）是中药槐花蕾的有效成分之一。槲皮素的3－羟基4－酮基，5－羟基4－酮基及3′，4′－二羟基均为可络合部位。

Quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E wereisolated from the flower of Acanthopanax senticosus. Searching bioactive natural compound and establishing quality evaluation standard arefocused on this experiment. Using cold immersing of 70% methanol to extract from flower ofAcanthopanax senticosus and integrating macroporous resin, silica gel column chromatography,sephadex gel to abstract. We obtained five monomer compounds (compoundⅠ-compoundⅤ),quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E. That five compoundswere determined by HPLC in eight parts of Acanthopanax senticosus (flower, root, leaf fruit,shoot, new shoot, phloem of shoot, skin of shoot), which provide science bases for establishingquality evaluation system and settle infarctate foundation.

本文以从刺五加花蕾中寻找具有重要生物活性的天然化合物和建立质量评价标准为主要目的，对其化学成分进行研究，采用70％甲醇冷浸的方法对刺五加花蕾中的成分进行提取，运用大孔树脂吸附色谱法、硅胶柱层析法以及葡聚糖凝胶柱层析相结合的方法进行分离，得到5种单体化合物，分别为槲皮素、槲皮素鼠李糖苷、金丝桃苷、紫丁香苷和刺五加苷E；利用高效液相色谱法对这5种化合物在刺五加花蕾中的含量进行了测定；考察了8个部位(花蕾、根、叶、果、茎干、新枝、茎韧皮部、茎皮)中这5种化合物的含量分布情况，并作一比较，为今后建立刺五加质量评价体系提供了科学依据，奠定了坚实的基础。

The method was successfully applied to separate and determine isoquercitrin, chlorogenic acid and quercetin in Inula britamica L.

将此方法应用于分离测定欧亚旋覆花中的异槲皮甙、绿原酸和槲皮素，取得良好结果。

By the quantitative analysis of flavonoids from the extract under acid hydrolysis condition, it is demonstrated that the level of isoquercitrin degrades along with the content of hydrochloric acid elevating and the level of kaempferol is reverse. Those reveal that there exist flavonoid conjugates whose aglycone is kaempferol in the leaves.

对青钱柳酸水解液中成分的测定表明，随着酸浓度的提高，异槲皮苷的含量下降，槲皮素的含量上升，山奈酚的含量也随酸浓度上升而增高，表明青钱柳中尚有以山奈酚为苷元的黄酮苷存在。

The results from statistical analysis of variance indicated that Bt toxin in Bt transgenic cotton had no negative effect on synthesis of flavonoids (mainly rutin, isoquercitrin and quercetin ).

显著性分析发现，外源Bt杀虫蛋白表达对棉花原有的芸香苷、槲皮素和异槲皮苷等抗虫黄酮类化合物不存在不利的影响。

Three flavonoids compounds: kaempferol *!, quercetin *, and isoquercitrin *# were isolated from the leaves of Cyclocarya paliurus Batal.

采用溶剂萃取及多种色谱技术分离纯化，根据理化性质和光谱分析方法鉴定其结构，确定从青钱柳中分离得到山柰酚、槲皮素和异槲皮苷 3个黄酮单体化合物，并用反相高效液相色谱法对其含量进行了测定，测得各自在甲醇和水提取物中的含量：山柰酚 0 。

An ionic liquid (1-ethyl-3-methylimidazolium tetrafluoroborate, 1E-3MI-TFB) modified micellar electrokinetic capillary chromatographic method was developed to separate and determine isoquercitrin, chlorogenic acid and quercetin.

建立了一种离子液体（1-乙基－3-甲基咪唑四氟硼酸盐，1E-3MI-TFB）修饰毛细管胶束电动色谱法分离测定异槲皮甙、绿原酸和槲皮素的方法。

Results showed a close relationship existed among the three flavonoid compounds with kaempferol content greater than quercetin and isoquercetin.

结果表明：青钱柳叶中3种黄酮类化合物质量分数紧密相关，其中山奈酚质量分数高于槲皮素和异槲皮苷。

RESULTS Kaempferol, quercetin and impurities were separated.

结果 槲皮素，山柰酚与相邻杂质能很好分离，其分离度分别为1.96和5.32，理论塔板数以槲皮素峰和山柰酚峰计算，分别为5121和6673；在5～100mg·L^-1内峰面积与其浓度均呈良好的线性关系（r=0.9994，0.9996），样品回收率分别为97.58％和105.67％；两成分含量以湖南张家界的最高而湖北赤壁最低。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。