植物原病

Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究，建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序；此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

Using universal primers (R16mF2/Rl and R16F2/R2) for 16S rRNA and primers (rpF1/rpR1) for ribosomal protein gene to detect phytoplasmas associated with of chinaberry tree witches'-broom from Yunnan and Guizhou Province by polymerase chain reaction.

根据植原体16S rRNA基因通用引物序列(R16mF2/R16mRl、R16F2/R16R2)及核糖体蛋白基因通用引物序列(rpF1/rpR1)，应用PCR技术对采自不同省份的苦楝丛枝病植原体进行检测，结果从两个植物上分别获得苦楝丛枝病植原体云南株系和贵州株系。

In order to explore the mechanism of the resistance to cucumber damping-off induced by plant growth-promoting rhizobacteria CH1, the physiological and biochemical method was employed to study the changes in activities of Phenylalanine ammonia-lyase, Polyphenol oxidase and contents of ferulic acid, chlorogenic acid, lignin, phenolic compound and flavonoid of phenolic metabolism in cucumber roots pretreated with plant growth-promoting rhizobacteria CH1 and infected by Pythium aphanidermatum.

为研究植物生长促生细菌CH1诱导黄瓜对猝倒病的抗性的作用机制，采用生理生化的方法，测定了黄瓜根部酚类物质代谢中苯丙氨酸解氨酶、多酚氧化酶活性动态及阿魏酸、绿原酸、木质素、总酚、类黄酮含量变化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。