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Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料,分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异,两种生境鸭跖草在铜胁迫下养分吸收和积累的差异,两种生境鸭跖草在铜胁迫下生理反应的差异,以及铜在鸭跖草细胞内的分布及结合形态研究,矿山鸭跖草差异表达的cDNA克隆,铜对鸭跖草种群遗传分化的影响,从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

The results of histochemical GUS assay showed that no expression of GUSint was observed in Agrobacterium, with transient expression as high as 100% in cotyledonary node of Brassica napus L. after inoculated for three days. It not only verified that the vector was successfully constructed, bur also laid a basis for further increasing the stable transformation efficiency of other Brassica plants, and rapidly clarifying the function and pattern of expression and regulation of a unknown gene.

组织化学染色结果表明,GUSint在农杆菌中没有表达,而在接种3d的油菜中可高效瞬间表达,其中在子叶柄中瞬间表达率高达100%,这一方面证实载体构建成功,另一方面也为进一步优化油菜及其它芸薹属植物转化体系及在油菜中快速研究目的基因的功能和表达调控模式奠定了基础。

CYP and P-gp mediated PP-CD interactions and the research methods have been discussed in this review.

本文综述了药物代谢酶CYP及药物转运蛋白P-gp介导的植物制剂—化学药物的相互作用及常用的研究方法。

The ethanol extract was dissolved with HCl, the acidic extract was basified to pH9-10 with NH_3-water and extracted with CHCl_3 to give total alkaloids. five alkaloids were isolated with column chromatography and preparative thin-layer chromatography, and three of them were identified as dehydrocorydaline、 corynoline and dihydrosanguinarine on the basis of spectral analysis MS,~1H-NMR,~(13C-NMR and physicochemical properties. All of them were found for the first time from this plant.

为研究灰绿黄堇的化学成分,本文将灰绿黄堇乙醇提取物采取酸提取、碱游离后萃取的方法得到总生物碱,然后利用柱色谱、制备薄层色谱等方法分离、纯化了灰绿黄堇中5种生物碱,并利用IR、MS、~1H-NMR、~(13)C-NMR等方法鉴定了其中的3种,分别为去氢紫堇碱、紫堇灵、二氢血根碱,均为首次在此植物中发现。

There are 23 species and 3 varieties Orobanche plants in China,most of which are distributed over north,northwest and northeast.The compounds of Orobanche were reported at phenylethanoid glycosides such as acteoside and isoacteoside,and acteoside has significantly protective againstα-galactose induced hepatic injury,which is better than Biphenyl dicarboxylate.

第一部分紫花列当化学成分及生物活性研究紫花列当Orobanche coerulescens Steph为列当科列当属二年生或多年生寄生草本植物,性温、味甘,生长于沙丘、干草原、砾石沙地和戈壁之中,寄生在菊科蒿属植物的根上,具有补肾、强筋之功效,用于肾虚、腰膝冷痛、阳痿遗精和神经官能症等疾病的治疗。

The review focused on recent developments concerning the chemical constituents of dipsacaceae species,their biological activities and clinical application.

从化学成分、药理作用和临床应用三方面综述了川续断科植物最近的研究进展。

Edaphic factors The physical, chemical, and biological characteristics of the soil that together form an important component of the habitat because of their influence on plant distribution.

土壤因子:包括土壤的物理、化学和生物特征,因其对植物分布的影响,共同构成了栖息地的重要成分。

Elsholtzia species ; Chemical constituent ; Traditional uses ; Labiatae

香薷属植物;化学成分;传统应用;唇形科

Fabaceae suggests that oligostilbenes are one of the main chemical components and active ingredients.

本课题组长期的研究经验表明,此类成分是锦鸡儿属植物中主要的化学成分和活性成分之一。

The author reviewed the research progress on the study of several Gibberella species of agricultural importance, which represent phylogenetic, biological and chemical diversity of this genus.

选取几种在农业生产上重要的赤霉属真菌来阐述该属在系统发生,生物学和化学上的多样性,并且比较了它们在分类学、遗传学、植物病理学和真菌毒理学方面的差异。

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