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棘球绦虫

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Platyhelminthes in many types of threats to people and livestock in health and life, such as schistosomiasis, Echinococcus granulosus and so on.

扁形动物中的不少种类威胁人畜的健康及生命,如血吸虫、细粒棘球绦虫等。

Recently, an unknown Echinococcus species was isolated from infected plateau pika (Ochotona curz-oniae) and Tibetan fox.

近年来,一种未知的棘球绦虫先后从高原鼠兔和藏狐中被分离出来。

Diagnosis of Echinococcus granulosus Infection in Dogs by Using Coproantigen Detection I.

粪抗原检测法诊断犬细粒棘球绦虫感染的研究Ⅰ。

Stagespecific expression of the 1433 gene in Echinococcus multiloculris[J].

多房棘球绦虫重组BCGEmII/3疫苗构建及其表达效率[J]。

Key words: Echinococcus granulosus;thioredoxin peroxidase;gene cloning;sequence analysis

细粒棘球绦虫;硫氧还蛋白过氧化物酶;基因克隆;序列分析

Objective To investigate protection of immunization with recombinant BCG-Em Ⅱ/3 vaccine of Echinococcus multilocularis and against challenge with Em protoscolexes.

目的探讨多房棘球绦虫重组卡介苗(BCG-EmⅡ/3)疫苗免疫对Em原头节攻击感染的保护性作用。

Hydatid cyst should be considered in the differential diagnosis of sciatica, especially when treating patients who live in or have emigrated from areas of the world where Echinococcus granulosus is prevalent.

坐骨神经痛的鉴别诊断应该考虑到包虫囊肿,非凡是对于那些居住或曾经去过棘球绦虫流行区域的患者。

Multilocularis in Chalong, Kalong,Dade and Chazha Townships in a district of Ganzi County,Sichuan Province,China. Method Questionnairing associated with the acquisition of canine echinococcosis was administered to dog owners.

分别对甘孜县达通玛藏族牧区查龙、卡龙、大德和查扎等4乡的犬主进行问卷调查,了解家犬感染棘球绦虫的相关因素。

Granulosus in Xinjiang and there was no nucleic acid sequence difference of Eg95 antigen among the protoscolex, oncosphere and adult worm of E. granulosus.

结论Eg95基因在新疆株细粒棘球绦虫不同发育阶段均有表达,其基因序列无差异。

Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.

方法根据Eg95基因序列设计引物,用PCR方法分别从新疆株细粒棘球绦虫3个不同发育阶段所构建的cDNA文库中克隆获得Eg95目的基因,将其克隆至pUCm-T载体、测序并进行序列分析。

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