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To make the classification of Helminthism of the livestock and poultry more convenient and short-cuting, the key system was set up by the programming language of Visual Basic. According to the morphologic characters of the orders, families and genuses of Cestoidea, Trematoda and Nematoda, a database was established. The correlative data can be searched easily and quickly.

为了使畜禽寄生蠕虫形态学分类更加方便、简捷,采用Visual Basic可视化的计算机编程语言,根据绦虫纲、吸虫纲、线虫纲和头虫纲的各目、科和属的虫体形态特徵建立数据库,通过资料查询系统,可方便快捷的对相关资料进行查询。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上处骨髓 5ml,经 percoll液分离后密度梯度离心,10'砌l密度接种培养,观察原代细胞的贴壁、增殖状况,细胞长满瓶底后进行传代培养;传至第三代细胞,重新消化后以500转/分钟轻度离心 5分钟,10V加 l接种,改用化学限定培养基代替完全培养基培养,倒置显微镜观察细胞生长情况及形态变化,甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM)中的蛋白多糖,免疫细胞化学染色检测ECM中*胶原的蛋白合成,RT干CR鉴定诱导细胞*胶原mRNA的表达。

RESULTS Based on the epilepsy typing method and Video-EEG data,54pa-tients(42.2%)were diagnosed for epilepsy syndrome in the128child patients,including25cases of benign epilepsy of children with centrotemporal spikes,3cases of childhood ab-sence epilepsy,1case of juvenile myoclonic epilepsy,5cases of frontal lobe epilepsy,13cases of West syndrome and7cases of Lennox-Gastaut syndrome.The types of epilepsy seizures in49(38.3%)patients were conformed,including10cases of tonic and/or clonic seizures,33cases of partial seizures,5cases of myoclonic seizures and1cases of atonic seizures.

结果 128例癫痫患儿中有54例(42.2%)进一步确定了癫痫综合征的诊断,其中儿童良性癫痫伴中央-颞区波25例,儿童失神癫痫3例,少年肌阵挛癫痫1例,额叶癫痫5例,West综合征13例,Lennox-Gastaut综合征7例;有49例(38.3%)可明确其发作类型,其中强直或阵挛发作10例,部分性发作33例,肌阵挛发作5例,失张力发作1例,另外有25例(19.5%)尚无法分类。

METHODS: 10 mL bone marrow was extracted from dog posterior superior iliac spine, heparin anticoagulation, diluted using Hanks juice, treated with 1.077 g/mL Ficoll solution 3 mL, and centrifuged at 2 000 r/min for 20 minutes. Karyocytes were absorbed to form white cloudlike layering interface, and then centrifuged twice using DMEM supplemented with fetal bovine serum, incubated at 12×104/cm2 at 37 ℃ in a 5% CO2 incubator.

于犬髂后上抽取骨髓液10 mL,肝素抗凝,Hanks液稀释后,加入1.077 g/mL Ficoll液3 mL,2 000 r/min离心20 min,吸取有核细胞形成白色云雾状的分层界面,用含胎牛血清的DMEM离心2遍,按12×104/cm2密度接种,于37 ℃、体积分数为5%的CO2培养箱内培养。

The important and perpetual anatomic landmarks looking for facial nerve in the course of operation are horizontal semicircular canal,digastric ridge,facial recess,cochleariform process,tarin foramen, arcuate eminence and foramen spinosum.

经乳突联合颅中窝径路行面神经手术时,二腹肌嵴、外半规管凸、砧骨短突、面神经隐窝、匙突、弓状隆起、孔、面神经管裂孔为该手术径路中寻找面神经的恒定的、重要的解剖标志。

The important and perpetual anatomic landmarks looking for facial nerve in the course of operation are horizontal semicircular canal,digastric ridge,facial recess,cochleariform process,tarin foramen, arcuate eminence and foramen spinosum.

二腹肌嵴、外半规管凸、砧骨短突、面神经隐窝、匙突、弓状隆起、孔、面神经管裂孔为该手术径路中寻找面神经的恒定的、重要的解剖标志。

METHODS: Through vestibular and columellar approach, the major alar cartilage and nasalis were repositioned to the normal anatomical positions, the deviated septum and columella were corrected by cutting the abnormal attachment of the orbicular muscle to the anterior nasal spine.

鼻唇畸形二期修复时做鼻翼缘前庭合并鼻小柱切口,矫正大翼软骨、鼻肌、鼻小柱及鼻中隔的异常解剖位置,离断鼻中隔的异常附着,部分患者去除多余的鼻中隔组织,并将鼻中隔软骨末端可靠固定在正确的位置,与软组织逢合固定,或与前鼻等骨性组织缝合固定,同时矫正偏斜的鼻小柱等软组织,通过鼻部软、硬组织的矫正,恢复鼻的正常形态。

Let us wish a wish, this Chih-hsueh be young of life of trip, such as he fancy of train sort Gao Ge Meng3 enter, the Tan concuss have no Ji.

让我们祝愿,这位志学少年的人生之旅,如他喜爱的火车般高歌猛进、坦荡无

Stratum granulosum: outside the Spinous layer, made up of cells further differentiated to cuticle cells, so it would lose the nucleolus and turn to the enucleated startum lucidum and horny layer after completely cornified to horny layer.

颗粒层:位于层之外,是进一步向角质层细胞分化的细胞,因此它起着向角质层完全角化后,便失去细胞核,而转化成无核的透明层和角质层。

Objective: To discuss the curative effect of craniofacial keratocanthoma which combine CO2 laser with Ho: YAG laser.

目的:观察CO2激光与Ho:YAG激光联合应用治疗头面部角化皮瘤的疗效。

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