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棉铃虫

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Using an anticoagulant it was possible to isolate plasma and intact haemocytes from Helicoverpa armigera larvae. PO and ProPO activities in plasma and haemocyte lysate were assayed when using bovine trypsin as an activator and L-DOPA as a substrate.

用抗凝剂从棉铃虫血淋巴中分离获得了血浆及完整的血细胞,以L-DOPA为底物,牛胰蛋白酶为激活剂,测定了血浆及血细胞裂解液中酚氧化酶及酚氧化酶原的活性。

And these two insects are sensitive to pro-toxin than toxin.2 Bt Cry1AcE toxins are composed of many peptides which have 60 kD weight, and all parts have biological activity to insect, not single part has activity.3 The mid-gut juice enzyme activity of M.

通过研究明确了以下问题: 1 Bt CrylAc毒蛋白对棉铃虫毒性较高,相比较而言对粘虫毒性较低,并且这两种昆虫对原毒素比毒素都要敏感一些。

Overexpression of several P450 genes from CYP9 and CYP6 families were reported to be associated with xenobiotic detoxification in H.

属于CYP9和CYP6家族的多个P450基因的过量表达与棉铃虫对外来化合物的解毒代谢有关。

Pbo significantly synergizes fenvalerate、cypermethrin and methomyl against Xihua field resistant strain ofHelicoverpe armigera , TPP can somewhat synergize pyrethroids also.

对西华田间棉铃虫抗性种群,Pbo能显著地增加氰戊菊酯、氯氰菊酯、灭多威的毒力,大幅度地降低其致死中量,TPP对两种拟除虫菊酯杀虫剂也表现一定的增效作用;对甲基对硫磷、甲胺磷,无论是Pbo还是TPP其增效作用均十分有限。

This paper was studied the derivation of seasonal variation from internal reason variation female sex pheromone and effects of exterior factors.

本文从棉铃虫性信息素的本身是否有变异及外界因素的影响来探讨季节性差异的原因。

The results demonstrated that esterase-mediated metabolism plays an important role in phoxim resistance in the BK77-R strain, and enhanced detoxification mediated by both esterases and oxidases is an important factor conferring broad cross-resistance to different classes of insecticides.

上述结果表明,酯酶解毒代谢在棉铃虫BK77-R品系对辛硫磷的抗性中起重要作用,酯酶和多功能氧化酶解毒作用增强是该抗性品系对不同类型药剂产生交互抗性的重要原因。

Four further single mutants ofN546(N546G,N546D,N546K,N546Δ), three double mutants and a triple mutant were constructed,and toxins were purified by isoelectric precipitation and an AKTA purifier 100 system.When bioassayed against H.armigera using purified toxins,N546ALC_(501.67μg/mLshowed 1.78 times increased toxicity than wide-type Cry1AcLC_(50 2.98μg/mL,and mutant N546D,N546K and N546Δshowed a great loss in toxicity.

对N546进一步突变,获得了突变子N546G、N546D、N546K和删除突变N546Δ及3个双突变和1个三突变,利用等电点沉淀法和AKTApurifier 100系统纯化了毒素蛋白,生测发现N546ALC_(50为1.67μg/mL对棉铃虫毒力比野生型Cry1AcLC_(50为2.98μg/mL提高了1.78倍,而N546D、N546K和N546Δ的毒力则降低了80~90%。

Among the midgut proteases in larvae, activities of chymotrypsin-like enzymes increased significantly with the increasing of Bt concentration, the activities of try...

棉铃虫中肠内蛋白酶中,类胰凝乳蛋白酶的活力随Bt蛋白浓度增大而显著升高;类胰蛋白酶活性在Bt蛋白浓度为 2 0 μg g时达到最高,随后开始下降。

Armigera. The mRNA expression levels of CYP9A12 and CYP9A17v2 in midgut were higher than those in the fatbody respectively.

以pYES2作为表达载体,在酵母W菌株中表达了棉铃虫细胞色素P450基因CYP9A12和CYP9A17v2,分析了这两个基因的异源表达产物对溴氰菊酯、氟氯氰菊酯、甲氰菊酯和联苯菊酯四种拟除虫菊酯类药剂的离体代谢作用。

In order to investigate the relationship between lipid raft and the formation of membrane pore by Bt β-endotoxins, the preparation and identification of lipid raft from the larval midgut of Helicoverpa armigera was performed.

为研究脂筏与Bt毒素在细胞膜上形成寡聚体膜孔的关系,本文对棉铃虫Helicoverpa armigera幼虫中肠脂筏的制备与鉴定方法进行了研究。

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