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棉铃虫

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By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段,用双脱氧链终止银染测序法测定了该片段的全序列,发现它含有一个完整。。。

The relationship between kanamycin-resistance and bollworm-resistance of transgenic cotton progeny was studied by using this method.

利用该方法对转基因植株后代卡那霉素抗性和棉铃虫抗性之间的关系进行了研究。

Assulta was studied in the present paper. Based on the successful reciprocal crosses, sex ratio and fertility of the hybrids in larvae, pupae and adults were investigated. In addition, the physiological mechanisms and genetic basis of host plant ranges of H.

在此基础上,利用解剖和观察等方法对子一代幼虫、蛹和成虫的性别比例和可育性进行了较为系统的研究;并利用比较的方法对棉铃虫和烟青虫食性广度的生理机制和遗传基础进行了初步研究。

In this paper,the behavioral,physiological, ecological adaption mechanism of H.armigera and H.assulta to their common host plant tobacco were studied comparatively,and the main results were summarized as follows:Electroantennogram response of adult to tobacco volatiles:(1) H.armigera and H.assulta elicited similar EAG responses to the tested 20 tobacco volatiles;both of them elicited significantly more sensitive to green leaf volatiles,aliphatic and aromatic compounds, than to monoterpene,sesquiterpene,heterocyclic compounds under lower concentration; while the similar EAG responses under higher concentration were detected,but most of them were not significantly different with each other.

本文从行为、生理和生态适应三个方面,对棉铃虫和烟夜蛾与烟草间的互作进行了比较研究,主要结果如下:成虫对烟草挥发物的EAG反应:(1)两种夜蛾对测试的20种烟草挥发物均可产生EAG反应;低浓度下对绿叶气味、脂肪族、芳香族化合物的EAG反应显著高于单萜类、倍半萜类和杂环化合物;高浓度下的EAG反应也表现出相似的总体反应趋势,但多数反应值间的差异未达显著水平。

In Xinxiang Aera of Henan Province in 1997, the occurrence of cotton pests were investigated in the Bt cotton and common cotton fields The result showed that the numbers of cotton bollworm egg were similarly in both fields, but that of survival larvae on Bt cotton were lower than that on common cotton The occurrence of cotton aphis was no difference but the spider mite were more heavy in Bt cotton field At the same time,control strategy against cotton pests were discussed for the Bt cotton

对河南新乡棉区种植的转Bt基因棉和常规棉棉田虫情进行了定点系统调查和大田普查,结果表明:棉铃虫在两种棉田棉花上的落卵量无明显差别,而幼虫存量差异显著,Bt棉棉花上幼虫存量显著降低;Bt棉对棉蚜的发生无明显的影响,而Bt棉上棉红蜘蛛的发生比常规棉略重,同时初步研究制定出种植Bt棉后的棉虫综防对策

The effects of tannic acid on GSTs specific activity were dependent on dosage and time.

用含0.005%单宁酸的饲料饲喂棉铃虫后,5龄和6龄幼虫的谷胱甘肽S-转移酶活性明显降低,分别为对照的59%和67%。

Combined with the characteristics of mobile network such as high covering rate, and simpleness of sending short message, GSM (global systems for mobile communication) is prevalent. With the support of hardware and software, the data investigated artificially from the field are sent to the GSM modem of the teledata server by normal format of short message.

结合农村GSM网络覆盖率高和手机短信使用简单等特点,采用硬件和软件结合技术,采用规范化的短信格式,将田间人工调查的数据,发送至远程数据库服务器上的GSM调制解调器,服务器端软件系统处理短信,将棉铃虫的信息,写入到相应的数据库中。

Was also observed and evaluated ,The poison symptoms of tropically treated M.separata were observed, following a similar pattern of nerve poisoning: excitation, convulsion, paralysis, and death. The effect of acetone extract from Ferula.ferulaeoidis on the development and hatch of the eggs and survival of hatched larvae as well as ovicidal activity was observed and estimated.

蛹的生长发育有抑制作用,中毒粘虫先后表现出兴奋、痉挛、麻痹等症状,最后死亡;对黄粉虫蛹生长发育的抑制作用类似于保幼激素(Juvenile hormone analogs, JHA)活性;对棉铃虫(Heliothis armigera Hübner)卵发育、孵化及初孵幼虫存活有明显影响,表现出一定的杀卵活性。

In the present study, differential display polymerase chain reaction was used to isolate three antennal -specific mRNA from the moth of cotton bollworm. Sequence analysis indicated that A4-1 clone was homologous to the glutathione S-transferase family of proteins.

利用差异显示PCR的方法分离得到了3条棉铃虫触角专一性的mRNA片段,在GenBank中进行同源搜索表明,克隆A4-1与昆虫谷胱甘肽-S-转移酶基因家族具有很高的同源性。

The contents of fat, glycogen, protein, RNA and DNA, in diapause pupa of Heliothisarmigera Hubner were determinated by ether extract method, anthroic ketone colorimetry,Folin-phenal method and ultraviolet absorption method respectively.

本文通过乙醚提取法、蒽酮法、Folin-phenal法和紫外吸收法分别测定了棉铃虫滞育越冬蛹在滞育期中脂肪、糖原、蛋白质、RNA和DNA含量的变化。

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