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检测板

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Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12~48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个/孔接种于6孔培养板中,使用含0μg/mL、150μg/mL咪喹莫特的培养液培养12~48h,收集贴壁生长的细胞制成单细胞悬液,PBS洗两次,分别加入5μL Annexin V/FITC和10μL浓度20μg/mL的PI溶液,混匀后室温避光孵育15min,流式细胞仪检测,结果用Cell Quest软件分析。

Methods: Use MLT(micro lymphocytetotoxicity test) and the SSOPH method detect HLA-A, B, and DRB1 antigen to 1,572 cord blood, some samples has question with PCR-SSP and the SBT method to redetect.

采用单克隆板和寡核苷酸探针杂交方法对15 72份脐血检测HLA -A、B、DRB1抗原,用PCR -SSP及SBT方法对有疑问的结果进行复核。

To study the effect of specific electromagnetic wave on the growth of microorganism,this paper uses TDP and TDP without radiation board to irradiate growing vintage saccharomycete and E.

为了研究特定电磁波对微生物生长的影响,用TDP和取下辐射板的TDP照射正在生长中的酿酒酵母菌菌液和大肠杆菌菌液,并检测菌液的OD值。

Hareware detection circuit takes up the space of board and augments the cost of design.

2反电动势过零点检测的硬件实现电路占用了板上空间、增加了设计成本、同时也需要较多的外部中断资源。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低,可能是由于其分子量较小,所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中,利用硫氧还蛋白融合方式对该抗原进行修饰,并在大肠杆菌中表达融合蛋白(Trx-B_2),纯化该蛋白后免疫小鼠及家兔,结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后,得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度,并从免疫后的兔血清中提取IgG包被酶联板,利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清,结果阳性率分别为87.5%和43.75%,符合率为71.88%。

Methods The examination specimen with the UuMh liquid media appraisal, the medicine sensitive pore plank performs the medicine sensitive experiment, Simultaneously vaccinates the mycoplasma appraisal agaragar culture medium to make the mycoplasma proof raise.

检测标本用UuMh液体培养基鉴定,药敏微孔板条做药敏试验,同时接种支原体鉴定琼脂培养基做支原体确证培养。

In this study, the characterization methods of barium nitrate were investigated by inductively coupled plasma atomic emission spectrometry, Flame Atomic Absorption Spectroscopy, and Ion Chromatography to detect the concentration of barium in powder and estimate the concentration of barium nitrate.

本研究利用热板加热消化发射药,并以硫酸钡沉淀法、感应耦合电浆发射光谱仪、火焰式原子吸收光谱仪及离子层析仪检测发射药中钡含量进而推算其硝酸钡含量。

My company specializing in the production of: sophisticated detection granite slab, granite platform, granite Jiaochi spent Shek Kong cubic feet, granite-foot, granite parallel regulation, to me granite, V-shaped granite blocks and measuring the surface, such as precision measuring instrument measuring tools, machine tools and various table, beams, rails, X-axis , Z axis, and other precision machine tools complement the granite mechanical components, test cast iron plate, flat-panel line, welding flat, T-shape groove assembly platform, the electrical test table, cast iron-foot, curved panels, to me, machine inquiry Seized equipment, machine tools test wand and machine adjustment Sizing Block, machine tools Earthquake Sizing Block, machine bed casting, mold Dianban, laboratory based flat-panel, slot Rail pad me, and so on.

我公司专业生产:花岗石精密检测平板、花岗石平台、花岗石角尺、花岗石方尺、花岗石平尺、花岗石平行规、花岗石方箱、花岗石V型块和测量表面等精密量仪量具,以及各种机床工作台,横粱、导轨、X轴、Z轴等与精密机床相配套的花岗石机械构件、铸铁检验平板、划线平板、焊接平板、T型槽装配平台、电机试验工作台、铸铁平尺、弯板、方箱、机床研检具、机床检验棒、机床调整垫铁、机床防震垫铁、机床床身铸件、模具垫板,实验室基础平板,槽铁垫箱等。

Objective: To study the method of ABO and RhD Blood Group Typing by microplate automatic detection system.

目的:探讨用微孔板代替纸板实现仪器自动化检测正反定ABO以及RhD血型。

Existing workers 123 (professional staff detected three, four computer technicians, engineers 4), I plant the production of glass fiber cloth (FRP cloth, grid layout, Wa Jibu, a board dedicated cloth, both inside and outside Qiangbu, etc.) by Users praise.

现有职工123人(专业检测人员3名,电脑技术人员4名,工程师4名)我厂生产各种玻璃纤维布(玻璃钢布,网格布,瓦基布,打板专用布,内外墙布等)受广大用户好评。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。