检测

Zhejiang saved haemal center to undertake active research and exploration to this, report as follows now: 1Blood is centralized the requirement that the system runs to haemal information in detecting 1.1 centralized the requirement of iformation flow safeguard in detecting is centralized detected success, iformation flow and content flow seasonable, effective delivering is crucial.

浙江省血液中心对此进行了积极的探究和探索，现报告如下：1血液集中化检测中对血液信息管理系统的要求1.1集中化检测中的信息流保障要求集中化检测的顺利进行，信息流和物流及时、有效的传递是关键。

Centralized the iformation flow in detecting should satisfy afore-mentioned requirements, the haemal information network that builds a stability besides the requirement is exchanged with satisfying the real time of data outside, still should use the haemal information that accords with uniform standard to run software in order to assure centralized detect the unity of flow and can accuse, in addition, still should build regional integrated database on this foundation in order to assure the normative sex of data and consistency.

集中化检测中的信息流要满足上述要求，除了要求建立一个稳定的血液信息网络以满足数据的实时交换外，还应使用符合统一标准的血液信息管理软件以保证集中化检测流程的统一和可控，此外，在此基础上还应建立起区域性的综合数据库以保证数据的规范性和一致性。1.2国外信息保障方面的经验欧盟、美国、日本等发达国家都对信息技术给予了充分重视和大量投入，如美国采供血机构中的红十字会系统为实现全美地区的集中化检测，于1991—19。。。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

The failure frequency of FCC and heavy-tailed property based worm detection algorithm is used as the kernel of the detection system.

在分析蠕虫传播机制基础上，基于入侵检测系统Bro的整体框架，以基于FCC(First Contact Connections，第一次连接)失败概率和重尾特性的蠕虫检测算法为核心，设计并实现了基于Bro的蠕虫检测系统。

Methods IUGR model were established by maternal protein-malnutrition. The mRNA levels of TLR4 in hepatics of IUGR rats at 3 weeks of age were analyzed by fluorescent quantization RT–PCR. TLR4 protein levels in hepatics were determined by Western blot. Hepatics TNF-αand IL-1βlevels were measured by ELISA.

采用孕期蛋白质营养不良法建立IUGR大鼠模型，应用荧光定量RT-PCR技术检测3周龄子鼠肝脏中TLR4的mRNA含量变化，Western blot方法检测子鼠肝脏TLR4的蛋白表达，并应用酶联免疫吸附法检测肝组织中肿瘤坏死因子-α和白介素-1β的水平。

Heptachlor epoxide was used as internal standard. Results The detection limit ranged from 0.2-15μgkg^(-1). The recoveries of most pesticides were good with the ranged of 70%-120% in 9 medicine crops.

结果 对48种中药材进行了检测；除敌敌畏、杀虫脒、磷丹及三唑磷外，其余农药的检测限都在0.2~15gkg^(-1)之间；以9种中药材为例检测，大部分农药的回收率在70%~120%之间。

Virtual instrument technology is utilized and products are focused in fault detection system for cable inflator/air pressure monitor system and, fault detection system for communication fibre and it was categorized as 2001 National Torch Plan.

公司应用虚拟仪器技术，专为电信系统研制生产的电缆充气机/气压监测系统、虚拟通信光纤故障检测系统，使得通信故障检测和故障定位更精确，管理更方便，虚拟通信光纤故障检测系统更被列入2001年国家级火炬计划。

The method is robust to the parameter and the input sequence of data, also can detect unknown intrusions.

基于特征的检测方法和基于指导的检测方法对已知的入侵行为有高的检测率

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

The development of this detection system is the first attempt to extend the application field of the NQR technique from laboratory environment to realistic environment, especially the field of the contraband detection.

正是由于这种检测方法良好的特异性并可实现在线检测，使它被认为是目前所有检测方法中较有效的一种，具有广阔的应用前景。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。