检测

Moreover, it cast off the fetters of numerous electric cable without the expensive wireless communication base depot support, and had the advantages of disassembling simple, unattended, low cost, low power consumption and the dynamic monitoring over the warehouse grain humidity and temperature change, has raised the important safeguard for the grain safe storage.

基于ZigBee模式无线通信网络技术的智能粮食综合管理系统，克服了传统检测系统有线检测元件在仓库布置大量检测连接电缆、装拆更换繁杂、易受雷击等弊端，甩开众多束缚电缆，不需要昂贵的无线通信基站支持、拆装简便、无人值守、低成本、低功耗，可动态监测仓库粮食温湿度变化情况，为粮食的安全储藏提了重要保障。

In the end of this thesis, the data independent detection model of exchange ratedata stream is built. Then, the algorithm provided in this thesis is used on the model to solve the problem of detecting the abnormal data block in exchange rate data stream. Experimental data are also provided.

最后，本文根据实时汇率数据流的特点，建立了数据点相互无关的实时汇率数据流的检测模型，然后采用本文提出的数据流的变化检测算法解决实时汇率数据流的异常检测问题，并给出了实验结果。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The title of my paper is the flame detecting system of Furnace Safeguard Supervisory System..The operation safety of the large scale boiler using coal or gas as the fuel is highly depending on the reliability and stability detections of the burning flame in the boiler.First,the paper summarizes the research background and the current situations on the flame detection system of FSSS in China and abroad, analyzes its future trend of market and technology,and then puts forward my research goals and expected results.and resolved problem.

大型燃煤燃气锅炉的安全运行在很大程度上依赖于锅炉炉膛火焰燃烧的稳定性和可靠性检测，论文首先综述了锅炉炉膛安全保护系统(Furnace Safeguard Supervisory System，以下简称FSSS)中火焰检测系统的国内外研究现状，分析了火焰检测系统的国内市场前景，提出了本课题的研究目标和需要解决的主要问题。

The detection device can detect the the free heights and elasticity of Brake Cylinder Buffer Spring

采用该方法设计的检测装置，可对制动缸缓解弹簧进行自由高检测和分段回弹力检测。

According to the specialty of the high-speed catenary s parameters inspection system, the inspection principle and approach are given in the paper.

本论文根据高速接触网参数检测的特点，给出了接触网相关参数的检测原理及方法，并设计了检测系统的软硬件组成方案。

Basing on flexible catenary system's parameters inspection system and according to the characteristics of rigid catenary system, the inspection items and approach are given in the paper.

本论文以现有的柔性接触网参数检测系统为基础，结合刚性接触网的特点，给出了刚性接触网检测项目和实现方法，并设计了参数检测与评价系统的软硬件组成方案。

Basing on flexible catenary systems parameters inspection system and according to the characteristics of rigid catenary system, the inspection items and approach are given in the paper.

本论文以现有的柔性接触网参数检测系统为基础，结合刚性接触网的特点，给出了刚性接触网检测项目和实现方法，并设计了参数检测与评价系统的软硬件组成方案。

Based on the overhead catenary system's parameter detection system and according to the characteristics of conduct rail, the paper presents the overall schema of the system including hardware and software.

本论文基于架空接触网参数检测技术，结合第三轨的特点，给出了第三轨相关参数的检测原理及方法，并设计了检测与评价系统的软硬件组成方案。

PC testing system software is to analyze the experimental data, draw and observe the testing process. The main control cell of the tester adopts the 16 bits MCU-MSP430F169 which has abundant resource and strong anti-jamming ability produced by the company of TI.

检测仪主要完成对空气压缩机的各种参数的检测，上位PC机检测系统软件则是对实验实据进行分析、绘图，方便观察检测过程中的数据变化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。