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Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法,检测不同浓度5-FU作用不同时间对Lovo细胞生长所产生的不同影响;光镜观察凋亡细胞的形态特点;流式细胞术检测凋亡细胞百分率;琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki-67及凋亡相关基因蛋白p53、Fas表达水平。

M treatment materials, and also tartaric acid and no maleic acid detected in G. v treatment materials. Inoculation also increased the content of organic acid secretion (increased by 19.80~56.87 mg/kg) of trifoliate root, and the application of AlPO4 greatly increased organic acid content (increased by 20.06~21.84 mg/kg); uninoculated plant root secreted a bit of organic acid; Mycorrhizal poncirus trifoliata seedlings excreted malic acid (42.87%), citric acid (39.22%) and oxalic acid (12.06%).

接种AM真菌的根系分泌的有机酸种类与对照有所不同,未接种处理枳分泌的有机酸有草酸、苹果酸、乳酸、乙酸、顺丁烯二酸和柠檬酸等6种,而接种G.m的则检测到草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸、丁二酸等7种,G.v处理的检测到酒石酸,接种处理均未检测到顺丁烯二酸;接种丛枝菌根真菌增加了枳根系分泌有机酸的量比未接种处理增加19.80~56.87 mg/kg,且施用AlPO4后有机酸含量显著增加(增加20.06~21.84 mg/kg;未接种植株根系仅分泌少量有机酸;接种植株根系分泌的有机酸以苹果酸(42.87%)、柠檬酸(39.22%)和草酸(12.06%)为主。

Sample pretreatment is an indispensable and weaker procedure in analyzing process, especially for bio-samples, because of the complex matrix, low concentration, interference to the determination or pollution to the apparatus. Samples must be suitable for testing and the analytes should be preconcentrated some times.

样品预处理是分析测试过程中不可缺少也是相对薄弱的环节,尤其是生物样品,因其成分复杂、待测物浓度低,易对检测干扰或污染仪器,检测时必须将其处理成适合检测的样品并将低浓度待测物富集浓缩。

Testing of relatives at risk: Newborn sibs of an individual with PKU who have not been tested prenatally should have blood concentration of Phe measured shortly after birth (in addition to newborn screening ) to allow earliest possible diagnosis and treatment.

对高危家属的检测:未进行产前检测的PKU患者的新生同胞兄妹应该在出生后不久就检测Phe血液浓度,以得到尽早的诊断和治疗。

A radiant energy path means comprising a first optical fiber waveguide means and a second optical fiber waveguide means; sensing means prismatically configurated so as to provide a direct reflective path from said first waveguide means, through said sensing means, to said second waveguide means, said sensing means being fabricated from material which absorbs a portion of a monochromatic radiant energy transmitted along said direct reflective path through said sensing means as a function of temperature; a monochromatic radiant energy source means optically coupled to said first optical fiber waveguide means, thereby introducing said monochromatic radiant energy into said first optical fiber waveguide means; and detecting means optically coupled to said second optical fiber waveguide means for detecting the intensity of the monochromatic radiant energy transmitted along said direct reflective path through said sensing means, the detecting means correlating the intensity of the transmitted monochromatic radiant energy with the temperature of the sensing means.

温度传感器组成一个联合:辐射能走了一首手段包括光纤波导手段和光纤波导第二手段;硒干旱prismatically手段配置以提供直接从反光路波导首次表示,次传感手段粗糙说,第二说波导手段飒传感手段伪造身份证从物质吸收了部分单色辐射能直接传送沿线说重 flective路径通过说温度传感功能为手段;一单色辐射能来源地说,就是先光光纤耦合波导手段次 ereby介绍说,首先说成单色辐射能光纤波导手段;一丁光学检测手段波导光纤耦合说二是加大检测手段的单色辐射恩顺沿说传染途径通过直接反光说传感手段,次五是加大检测手段的传播相关单色辐射能与温度传感手段。

Objective:(1) To compare the sensitivity, specificity, and positive prognosticative value in the diagnosis of rheumatoid arthritis by examining antifilaggrin antibody, anti-cyclic citrullinated peptide antibody and rheumatoid factor respectively simultaneously.

目的:①检测类风湿关节炎病人血清抗聚角蛋白微丝蛋白抗体、抗环瓜氨酸肽抗体及类风湿因子,比较单独检测和联合检测对RA诊断的敏感性、特异性、阳性预测值。

Results The Southern blot result showed that 4 among 188 goats produced by pronuclear microinjection had integrated hEPO gene.

结果在原核注射获得的188头羔羊中,经Southern blot法检测有4头羊含有hEPO基因,其中3头为母羊,1头公羊于出生后20d死亡;3头转基因母羊hEPO基因的拷贝数分别为1、10、2;Western blot检测结果显示转基因羊乳中的hEPO分子质量为32kDa;MTT法检测结果表明,在泌乳10d的3只转基因羊乳汁中,每毫升乳汁中hEPO活性分别达到1.17×102IU、1.90×104IU、1.91×104IU。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法,将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中,对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测,对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白,并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测

In chapter 5, the integrated flow of colligation measurement system of concrete pump is designed to measure the systems integration interface by using GUI interface of Matlab.The real time working conditons are inspected by processing of filter, examining data, judging working conditions, calculating pumpage and analyzing error. In Chapter 6, the research of this paper is summed up and forcasted.

第五章设计了混凝土泵车实时排量检测系统集成流程,用Matlab中的GUI界面设计实现了泵车实时检测系统集成界面,把采集来的信号读取到界面后进行滤波处理、数据查看、实时工况判断、实时排量计算和误差分析,检测泵车泵送系统和液压系统实时工况。

A simple digital device for detecting human balance function is described. The device acquires data of a shaking body of quizzee using a micro sensor for measuring rotator, transforms the data into digital pulse signal, and displays the metrical results through digital display and an audio annunciator.

一种用于人体平衡功能检测的简易式数字检测系统,它通过旋转型传感器采集被测人体的晃动信息,并转化成数字脉冲信号,配合数字显示和声音报警来显示检测结果。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。