检测

The application of nonextensive distribution model for sea clutter modeling and small targets detecting is improved. Surrogate data method is used to analyze nonlinear character of sea clutter.

本文分析了海杂波在统计模型建模后运用最大似然比检测准则下，很难检测出弱小目标的缺陷，改进了非广延分布模型在海杂波建模和弱小目标检测领域的应用。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

But it is doubt that the speciality and stability for the outcome of detecting BDV ORF I genome.

但对另一编码P40蛋白序列的检测结果的特异性和稳定性还不肯定，我们通过对先前检测BDV ORFⅡ基因片段为阳性或阴性的神经精神病人和健康献血者进行了BDV ORFⅠ基因中部片段的检测。

The biochemistry and image analyzing were used to investigate the effects of the PDGF-AA at high concentration on the osteoclastic bone resorption in the osteoblast-osteoclast co-culture system.

在高浓度PDGF-AA的作用下，应用生化检查和图象分析技术，检测PDGF-AA对成骨细胞-破骨细胞共育体系中破骨细胞骨吸收功能的影响；应用免疫荧光检测破骨细胞合成抗酒石酸酸性磷酸酶的变化；应用免疫组化和RT-PCR技术检测骨保护素配体的表达。

M, wide linear range (0.78μM~0.50 mM) and short response time (within 7 s). Other oxidable amino acid would make little interference signals in the cysteine detection.

此外该电极对半胱氨酸的检测有低的检测下限(0.26μM)和短的响应时间(7s内)，其他可氧化氨基酸在测量电位下对半胱氨酸检测几乎不产生干扰信号。

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

And detected precision still is restricted certainly, at present each countries develop maritime petrolic actively high-tech detects technology, better detect method still is phreatic water examination.

而且检测的精度仍然受到一定的限制，目前各个国家都积极开发海上石油的高科技检测技术，比较好的检测办法仍是潜水检查。

Reverse-transcription PCR assays were used to detect the mRNA expression level of related regulatory genes such as p15, p16, p21, p27, p57, surviving, cyclin B, cdc2 and chk1, and Western blot assay to detect the level of protein expression and phosphated change such as survivin, cdc2 and chk1. Results:(1) K562 cell arrested on G2/M phase were obviously increased after co-cultured with 2 to 10μmol/L Arsenic trioxide for 24 hours. The ratios of control group, 2μmol/L, 5μmol/L and 10μmol/L groups were 22.6±3.4%, 27.2±2.3%, 43.8±4.5% and 36.7±4.1%, respectively.

体外培养K562细胞，以不同浓度AS_2O_3作用不同时间后采用PI染色、流式细胞仪检测药物作用后细胞的周期分布改变；逆转录酶多聚酶链扩增方法检测细胞周期相关调节基因(p15、p16、p21、p27、p57、survivin、cyclinB、cdc2、chk1)的mRNA表达变化；Western blot方法检测细胞周期相关调节蛋白表达及磷酸化改变(survivin、cdc2、cdc2-p、chk1)。

Serum total cholesterol was determined by isopropanol withdrawing and ferric-glacial acetic acid -sulfuric acid developing method, serum triglyecride by isopropanol withdrawing and acetylacetone developing method,serum high density lipoprotein-cholesterol by magnesium phosphotungstate mehod, respectively. Serum low density lipoprotein-cholesterol was calculated by the equation: LDL-C=TCh--0.2 TG.

采用异丙醇抽提、三氯化铁-冰醋酸-硫酸显色法检测血清总胆固醇，用异丙醇抽提、乙酰丙酮显色法检测血清甘油三酯，用磷钨酸镁法检测血清高密度脂蛋白-胆固醇，用等式LDL-C=TCh--0.2TG计算血清低密度脂蛋白-胆固醇。

Method; A rat model of acute cerebral ischemia was built by photochemically initiated thrombosis . Vegf - mrna was observed with RT - PCR. Vegf and cell proliferation were observed with immunohischemistry.

用光化学法制作局部脑皮质梗死模型，针刺治疗3天后，应用RT—PCR检测梗死边缘区 VEGF mRNA的表达，用免疫组织化学方法检测VEGF表达和细胞增殖，用激光多谱勒检测造模后 2 h和72 h的血流情况量。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。