检测

Methods 2BS cell lines were treated with Tuber Fleeceflower Root serum. The activity of c-myc mRNA was detected by semi-quantitive RT-PCR. A TRAP-ELISA method was used to measure the telomerase activity.

采用含何首乌血清对2BS细胞株进行处理，用黄嘌呤氧化酶法检测SOD活性，RT-PCR检测c-myc mRNA表达，TRAP-ELISA检测端粒酶活性。

The thesis focuses on gene diagnosis of diseases, and six new homogenous fluorogenic probes are developed.

本文致力于疾病的基因诊断研究，开发了六种新型的均相荧光基因检测探针，并以病人血清中的乙型肝炎病毒为检测对象，对上述六种探针的检测效果进行。。。

The thesis focuses on gene diagnosis of diseases, and six new homogenous fluorogenic probes are developed. Using hepatitis B virus in human sera as targets, the detection effects of these probes are discussed.

本文致力于疾病的基因诊断研究，开发了六种新型的均相荧光基因检测探针，并以病人血清中的乙型肝炎病毒为检测对象，对上述六种探针的检测效果进行了评估。

Firstly, the paper, combining the characteristic of synchronous pulse bursts and inhibition with the modified PCNN model, presents a way of finding the foveation points in the images adaptively and effectively, and simulates the human vision system. Secondly, PCNN is extended to PCNNs, based on the properties of information couple and transmission, an algorithm that is used to fuse images of the same target got by several sensors to an image is presented to simulate the human vision system. Thirdly, combining the properties of synchronous pulse bursts, capture, and transmission and competition of waves, the paper presents two ways of classification, one is an algorithm based on the properties of neuron to capture and inhibit to classify the data taking on any complex unlinear distribution robustly, the other is based on the restricted distance and modified of the former to remove the influence of inferior samples in classification; Finally, based on the accumulative difference pictures, and the forming and transmission of PCNN wave, selecting and controlling the direction of autowave by connecting the neighbouring neurons selectively, the paper presents a way to simulate the tracks of moving object and detect the moving direction.

首先结合PCNN的同步脉冲发放和侧抑制特性，提出了基于改进型PCNN的图像凹点检测算法，该算法是一种自适应而有效的图像凹点检测方法，并且较好地仿真了人类视觉系统；然后，结合信息传递和信息耦合特性，将PCNN扩展成PCNNs，提出了一种基于PCNNs的图像融合算法，能够将多个传感器获取的同一目标的图像信息融合到一幅图像中，有效模拟了人类视觉系统；另外，结合PCNN的同步脉冲发放特性、捕获特性和波的传播竞争特性，开拓地将PCNN用于模式分类中，提出了基于耦合神经元点火捕获／抑制特性的分类方法和改进的约束距离下的PCNN分类方法，前者可实现对样本空间中任意复杂分布训练样本的稳健非线性分类，而后者能够消除训练样本中刺点对分类的影响；最后，结合累积差分图像思想、PCNN波的形成与传播特性，通过各神经元之间连接取向来选择与控制自动波的流向，将PCNN用于运动视觉分析中的运动轨迹模拟及运动方向检测。

Results The positive percentage in superficial gastritis, deep gastritis, gastritic ulcer, duodenal ulcer, chronic atrophic gastritis and gastric cancer, were 70.3, 72.2, 77.7, 69.2 and 25 respectively by URT, 59.2, 77.8, 71.4, 70.7, 75 and 78 respectively by PCR, and 48.1, 61.1, 70.3, 70.7, 66.7, 66.6 and 72.2 respectively by cagA gene probe detection. Conclusions Statistics suggest that cagA+ H.

结果 浅表性胃炎、深度胃炎、胃溃疡、十二指肠溃疡、慢性萎缩性胃炎及胃癌病人的检测结果是：尿酶检测阳性百分率为：70.3、72.2、77.7、69.2、25；PCR阳性百分率分别为：59.2、77.8、71.4、70.7、75、78；基因探针杂交检测阳性率分别为：48.1、61.1、70.3、70.7、66.7、66.6、72.2。

In this paper, methods for density detection, viability assessment and species characterization are introduced, and performances are evaluated and compared. Finally, a package of analytical methods for systematic research of Cryptosporidum and Giardia are suggested.

对两虫问题的系统研究离不开有效的检测手段，分别对两虫的密度检测、活性分析和种类鉴定方法进行了系统介绍，并对各种检测方法的优缺点进行说明和比较，进而提出了两虫的成套分析方法。

Methods MTT assay for detection of single use of Adriamycin and combination of Adriamycin with gleditsia saponin on the Role of the SMMC-7721 cell survival rate; detection of mRNA expression of bcl-2 by PCR method; detection of protein expression of bcl-2 by Western blotting method.

MTT法检测单独应用阿霉素或阿霉素与皂角皂苷联合作用的SMMC-7721细胞的生存率；RT-PCR检测bcl-2 mRNA表达；Western blotting检测bcl-2蛋白的表达。

The system could acquire images of sample at any beginning position, and if only the parameters of image have been set properly, the system could automatically inspect the headplate packaging. An adaptive morphological filter based on 2-D image entropy is used to preprocess the original images.

在系统硬件设计中，采用计算机与单片机通信的方式来控制作为载物平台的高精度二维运动系统的连续操作运行，我们可以从不同的初始位置对被检测样品进行图像采集，也可以预设图像采集参数和检测流程实现系统自动检测。

The antibodies of measles,diphtheri a and pertussis were detected with ELISA,agglutination and indirect hematocyte a gglutination test,respectively.

麻疹抗体检测用ELISA法，百日咳抗体检测用凝集试验，白喉抗体检测用间接血凝试验。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。