检测

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

This invention be able to real time detect, display and deliver data to computer for display and store. This invention not only can apply to detecting led component in crystal engine gutter tail's solder, also can apply to internal heating type multistage series vacuum delead kiln, to solve Pb content automatic detection problem of key equipment's Sn - Pb alloy.

本发明可实时检测、显示，以及将数据送上位机显示和存储，既可应用于结晶机槽尾焊锡中铅成分的检测，还可以应用于内热式多级连续真空脱铅炉中，以解决两关键设备的重要参数Sn-Pb合金中Pb含量自动检测问题。

The repeatability, recovery, and detection range of EBC method and chemical radiation method could meet the requirements of routine analysis. However, they could not measure the true diacetyl content.

EBC法和化学发光法的重复性、回收率和检测限可达到常规分析的要求，但检测结果并不能反应双乙酰的真正浓度，可适合检测要求不高和常规分析。

Asymmetric over-introgression of the indica alleles on chromosome 7 and many specific regions on other chromosomes in the japonica genetic background is consistent with the general trend observed in the RIL and F_2 populations of the same cross, suggesting a high genetic load in the japonica gene pool, and japonica genome experienced a very severe bottleneck by artificial domestication.2 Genetic background effect on QTL expression of grain weight and its related traitsGenetic background effect on main-effect QTL and digenic epistatic QTL mapping for GWT and its related traits including GL, GW and GT was dissected by a large set of reciprocal introgression lines and recombinant inbred lines derived from Lemont and Teqing in Beijing environment.

籼粳相互导入具有不对称性，粳稻向籼稻背景的平均导入频率与期望值相仿，相反籼稻向粳稻背景的平均导入频率大大高于理论预期，位于第7染色体和一些其它染色体的特殊区段存在籼稻等位基因的超导入现象，在来自相同亲本的重组自交系和F_2群体中也观察到同样的导入趋势，表明粳稻基因组具有较大的遗传负荷，粳稻品种在人工驯化过程中曾经历过一个非常剧烈的遗传瓶颈。2水稻籽粒相关性状的主效和互作QTL表达的遗传背景效应利用Lemont和特青培育的双向导入系和重组自交系群体，在北京环境下定位影响千粒重及其相关性状如粒长、粒宽和粒厚的主效和互作QTL，分别在特青背景导入系、Lemont背景导入系和重组自交系下检测到影响千粒重及其相关性状的主效QTL分别为33个、27个和13个，互作QTL分别为4个、8个和23个，其中仅有6个主效QTL(QGwt5，QGl1，QGl3b，QGl4，QGw7和QGw8)在籼粳两个不同背景下共同表达，3个主效QTL(QGwt5，QGwt7a和QGt5)在导入系和重组自交系中共同表达，分别占检测到影响这些性状QTL总数的11.1%和4.3%，未检测到在3个背景下相同表达的互作QTL。

ELISA method was used for comparison. Results The positive rate of DIGFA in testing the serum from 50 infected rats was 96.0%(48/50). The negative rate of DIGFA in healthy rats was 100%(50/50). Cross reaction with Enteroclysis (13 samples) and Tapeworm (11 sqamples) was not observed. The cross reaction rate in 18 Strongyloidiasis rats was 5.6%. The sensitivity and specificity of DIGFA is comparable with ELISA (96.7%).

结果 用DIGFA检测广州管圆线虫实验感染鼠血清50份，其阳性检出率为96.0%，50份正常鼠血清的阴性符合率达100%，DIGFA分别检测蛲虫阳性鼠血清13份，绦虫阳性鼠血清11份和粪类圆线虫阳性鼠血清18份，前二者均为阴性，后者交叉反应率为5.6%；DIGFA与ELISA平行检测30份广州管圆线虫阳性鼠血清，两法符合率达96.7%。

Combining the general detecting scheme with the method of quality management, the on-line detecting quality for the shape and position tolerance of the epicyclic gearbox and the final product detecting quality have been well controlled, so that high quality product rate has been increased.

用一般的检测方案和质量管理相结合的方法，对行星轮箱体形位公差的在线检测质量和成品检测质量进行控制。

Furthermore, K562 cells transfected by lentivirus plasmids were induced into the erythropoietic cells and the erythropoietic differentiation of K562 cells were examined by benzidine staining, immunocytochemistry and RT-PCR.

进一步对SOCS-3基因沉默后的K562细胞进行了诱导分化，并采用联苯胺染色法检测K562细胞向红系分化比例变化，免疫荧光染色检测细胞表面抗原的变化，RT-PCR 检测造血相关基因的变化。

Methods: the experimental condition for preparing the gene chips was studied and 7 fragments were screened from GenBank as probes dotted on chips to make HBV genotyping gene chips, many examinatorial experiments were done to evaluate the quality of this gene chip, and to analysis its detection characteristics.

对大量HBV各基因型序列对比分析后，设计7支探针，用芯片点样机点样于经预处理的玻片上制成HBV基因型检测芯片；对芯片检测的实验条件进行研究探索；进行芯片质量性能的考察实验，分析其检测特性。

Methods The mobile population from some provinces which were heavily endemic areas of filariasis was observed to detect the filarial infection by filarial-specific IgG4 testing kit.

方法应用丝虫特异IgG4试剂盒，检测重点省原丝虫病流行区外来流动人口，同时检测本市居民和6~12岁儿童特异IgG4，阳性者进行病原学检测。

On the basis of electrical network characterization, Chapter 5 presents a new principle to detect PCB's fault first time. Three methods of online test for electrical network characterization are described in detail. Then, a hierarchical, combinatorial approach is firsfly presented. The test efficiency of this method is studied also. The experiment result shows that the new approach can greatly enhance the test efficiency of fixtureless tester under the requirement of 100% test for PCB's connections.

本文第5章利用集成电路的输出特性，首次提出了一种新的印刷电路板连接故障的检测原理，给出了三种印刷电路板电气网络输出特性的在线测试方法；在此基础上，首次提出并实现了基于飞针式在线测试仪的层式组合测试思想，研究了这种方法的检测效率；应用实例表明这种方法在不降低对印刷电路板连接故障100％测试的要求下，可以有效地提高飞针式在线测试仪的检测效率。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。