检测

The soluble peptidoglycan is gained by short dose benzylpenicillin sodium and sephadex G-100; the SLP reagent kit revealed the prepared soluble peptidoglycan is peptidoglycan; the limulus test showed that the content of LPS in the prepared soluble peptidoglycan(10ng/ml) is lower (0.01ng/ml); the improved LOWRY reagent kit showed the content of protein in the prepared soluble peptidoglycan(1.0mg/ml) was 0.06mg/ml; the optimization condition is 0.75mg/ml of the dosage of benzylpenicillin sodium,1h of the incubation time of benzylpenicillin sodium addition and 5×108CFU/ml of the bacterial concentration.

应用青霉素诱导和Sephadex G-100凝胶柱成功分离得到金葡菌可溶性PGN；所制备的可溶性PGN经SLP试剂检测结果为阳性；动态浊度鲎实验检测制备的可溶性PGN(10ng/ml)中LPS含量小于0.01ng/ml；改良LOWRY试剂检测可溶性PGN(1.0mg/ml)中蛋白含量为0.06mg/ml；青霉素诱导和Sephadex G-100凝胶柱法提取金葡菌可溶性PGN的最佳条件是：青霉素的使用浓度为0.75mg/ml，菌液浓度为5×108CFU/ml，加入青霉素后孵育时间为1h。

Methods Twenty Wistar rats were divided into 2 groups: a normal control group and a DN model group. We determined the 24 h proteinuria and other biochemic indexes, measured the ultrastructures of podocytes with electronic microscope, and detected the expression of nephrin and podocin with immunohistochemical technique and RT-PCR at the 4th and 8th weeks.

将20只大鼠随机分为正常对照组和糖尿病肾病大鼠模型组，于第4， 8周检测24h尿蛋白量等生化指标、用免疫组织化学和RT-PCR方法检测肾皮质nephrin， podocin蛋白及mRNA 表达，用透射电镜检测足细胞超微结构变化。

Methods:Radioimmunoassay method was used to detect the levels of IL-6 in the cerebral spinal fluid.nephelometry method was used to detect the levels of albumin in the CSF and bromocresol green method was detect the levels of albumin in the serum.

用放射免疫法检测IL-6、溴甲酚绿法检测血清白蛋白、免疫比浊法检测脑脊液白蛋白。

1Livestock Research Institute, Council of Agriculture(2)Department of Animal Science, National Pingtung University of Science and TechnologyGenetic variation in the N-acetylglucosamine 6-sulfatase (G6S) gene is the key role in caprine Mucopolysaccharidosis IIID.

本研究建立山羊黏多醣症遗传缺陷之单股构型多态性基因型检测方法，应用此方法来检测乙醯醣胺氨基硫酸酶(N-acetylglucosamine 6-sulfatase， G6S)基因型不再需要使用限制酶，可节省检测时间、成本与人力。G6S基因的遗传变异在山羊黏多醣症第三型扮演重要的角色。

This dissertation fell to two hierarchical and four sections. Firstly, the existence of OH, the active substance, was detected in the catalytical and oxidizing system of the nano-semiconductor by ESR, which provided a useful analytical and detective method for the detection of the OH .

本论文分为二个层次，首先利用电子自旋共振的方法检测了纳米半导体光催化氧化体系中，活性物种羟基自由基的存在，为羟基自由基的检测和揭示纳米半导体氧化物的氧化机理提供了有用的分析检测手段。

The effects of cholic acid concentration and pH of the buffer on separation were studied, and the influences of organic additives such as methanol, acetonitrile, isopropyl alcohol were also examined.

该实验表明电化学检测同样可用于手性物质的分离，从而扩宽了电化学检测的应用范围，并为对映体的定量合成和纯度测定找到了灵敏的检测方法。

38 patients with nasal polyp and 15 control cases were chosen. Their IL-5 concentration in tissue homogenate were measured by enzyme-linked immunosorbent assay. All samples were stained with Chromotrope 2R histochemical and SP immunohistochemical method separately to examine eosinophil and IgE+ cells in nasal polyp.

方法采用免疫组化SP法检测38例鼻息肉，15例正常鼻腔粘膜组织中IgE阳性细胞表达情况；采用酶联免疫吸附试验检测以上病例中IL-5含量；采用Chromotrope 2R特染方法检测以上病例中Eos浸润情况。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

Activities of chymase and ACE were tested by radioimmunological method. Expressions of phosphoric extracellular signal regulated kinase 1/2 (p-ERK1/2) in hearts were measured by western blot. Results: As compared to the control group levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in those of APS group.

心肌组织的血管紧张素Ⅱ含量；RT-PCR法检测心肌糜酶和血管紧张素转换酶 mRNA表达；放免法检测chymase和ACE的活性；免疫蛋白印迹法检测心肌磷酸化的细胞外信号调节激酶1/2(p-ERK1/2)含量。

An otherwise healthy 12-month-old girl presented for evaluation of reduced abduction of the left eye detected at 6 months of age. The remainder of the examination was unremarkable. A special MRI sequence-fast imaging employing steady-state acquisition-visualized the right but not the left sixth nerve cisternal segment. This is the first reported use of the MRI FIESTA sequence to diagnose aplasia of the sixth cranial nerve.

应用快速平衡稳态取像核磁技术检测第六颅神经发育不全：本研究首次报道利用 MRI FIESTA 诊断第六颅神经发育不全，一例12个月的婴儿其他各项指标正常，在6个月时发现左眼诱导注视减少，其他的检查未见明显异常，利用 MRI FIESTA 可检测到右侧第六颅神经，但左侧部分检测不到。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。