梯度法

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。

Objective To establish a new method for isolating serum β-lipoprotein subfraction s using second double gradient polyacrylamide gel electrophoresis.

目的建立一种分离血清β脂蛋白亚组分的新方法———二阶双梯度聚丙烯酰胺凝胶电泳法。

The molecular weights of the two subunits were shown 142 KD for the larger one and 47 KD for the smaller one, respectively, on SDS—polyacrylamid electrophoresis. The molecular weight of the native vitellin was determined as 205 KD on native gradient polyacrylamid gel electrophoresis.

用蒸馏水沉淀法从东方粘虫Mythimna seperata新鲜卵中提取卵黄蛋白，SDS-PAGE分析结果显示，粘虫的卵黄蛋白由大小两个亚基组成，大亚基分子量为142KD，小亚基分子量为47KD，线性聚丙烯酰胺梯度凝胶电泳结果显示，卵黄蛋白的天然分子量为204KD。

MethodsChromatographic fingerprint of Amomum villosum,Amomum chinense and Alpinia zerumbet was determined by RP-HPLC and the gradient elution mode was applied in chromatographic separation,data were analyzed by Fingerprint Similarity Evalution Software to compare the similarity of samples.

方法采用RP-HPLC法，梯度洗脱，测定阳春砂、海南假砂仁及艳山姜的指纹图谱，并作相似度比较分析。

Experiment results show as follows in the processing of semiconductor etching, chemical heat centers in the semiconductor material, and is convected gradiently in all directions; the vertical heat convection velocity is obviously swifter than the horizontal; the process of heat convection can be real- timely recorded, and the heat convection situation can be gotten in any small area at any time by infrared camera which makes the analyse and understanding of heat convection characteristic in the processing of wet etching more intuitionistic.

实验结果表明：在半导体腐蚀过程中，化学热以半导体材料为中心，向四周梯度状对流；向上的热对流速率明显大于水平的热对流速率；红外热像仪可以实时纪录热对流过程，并获得任意时刻、任意小区域的热对流情况，使得对湿法腐蚀过程中热对流特性的分析和理解更加直观。

According to the shortage of only considering the phase change of emitter and no refer to the amplitude change, based on the patterns of radar and decoy, using equal phase gradient method, while considering the influence about waviness of phase and amplitude of each, the decoying formula of the coherent decoy system is deduced, a damage probability model is drawn out.

针对目前多点源诱偏效果评估过程中，仅考虑各点源相位分量变化，未考虑幅度分量在空中变化的不足，基于雷达和诱饵的具体方向图，运用等相位面梯度分析法，同时考虑各点源辐射信号的相位和幅度波动的影响，对相参多诱饵系统的诱偏误差公式进行了推导。

Coli BL21 (DE3) plysS for expression under induction of IPTG. The expressed product in a form of inclusion body was denaturalized with urea, purified by nickel ion chelate affinity chromatography, renaturalized by gradient dialysis and identified by Western blot.

coli BL21(DE3) plysS进行诱导表达，采用Ni(上标 2+)螯合亲和层析法纯化经尿素变性的包涵体，梯度透析复性纯化的目的蛋白，并进行Western blot鉴定。

This principle eradicates local minima or invalid solutions caused by consistent reduction of the energy function via the usual gradient descent method.

利用这一理论，可以克服由于梯度下降法所导致的网络能量函数总是下降，从而使网络陷入局部极小值或不可行解的现象。

For the determination of five carbamate pesticide residues by high performance liquid chromatography with gel permeation chromatography purification, sample was extracted with acetonitrile and purified with gel permeation chromatography. Methanol-water was used as the mobile phase by gradient elution, flow rate of 1.0mL/min, using a fluorescence detector and quantitating with external standard method.

凝胶渗透色谱-液相色谱法测定5种氨基甲酸酯类农药残留中，实验采用乙腈提取，凝胶渗透色谱柱对样品进行净化，以甲醇-水为流动相，进行梯度洗脱，流速1.0mL／min，荧光检测器检测，外标定量测定。

The results showed that plenty of the purified DPV virions were obtained after the cell lysate was condensed by 5-fold by ultrafiltration,and the concentrated DPV fluid was ultracentrifuged with 300g/L sucrose cushion method and 300-600g/kg sucrose density gradient centrifugation methods.

结果显示，用切向超滤法将病毒培养物浓缩为原体积的1/5后，再采用300g/L蔗糖垫底超速离心和300～600g/kg蔗糖密度梯度离心的方法进行纯化，可获得大量的纯化病毒粒子。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。