梯度法

Suppressor is the key part for IC instrument, and developing a high performance suppressor for IC instrument is quite important to gradient ion chromatography, to analysis of some ions which have strong affinities with the functional groups located on the surface of the pellicular ion exchange column packing.

抑制柱是离子色谱仪的一个核心部件，研制具有较高抑制容量的抑制柱对于离子色谱法的梯度淋洗、对于在分离柱上保留时间长的离子分析具有重要的意义。

An improved purification procedure for satsuma dwarf viruswithout sucrose density-gradient centrifugation was developed,by whichglobular SDV particles about 25 nm in diameted were observed with elec-tron microscopy,using only 100-200 g of Physalis floridana-infectedleaves as the virus source.

本文提出的温州蜜柑萎缩病毒提纯法，仅用100～200克病洋酸浆叶，且无需通过蔗糖密度梯度离心，在电镜下亦能看到中量直径约25nm 的球状病毒颗粒，粗提纯制剂OD_(860)/OD_(280)平均值为1.28，OD_(260)/OD_(240)平均值为1.15，初步显示出SDV 特征性紫外吸收曲线，提纯病毒产量约为4.5～11mg/kg病叶。

ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

选择健康杂种犬36只，无菌抽取犬骨髓液10 mL，经Percoll液密度梯度离心后贴壁法体外培养骨髓间充质干细胞，传2代后行5-氮胞苷诱导，5-溴脱氧尿苷标记备用。

Methodsa gradient hplc system was used in the quantitative analysis of the danshensu ,protocatechuic aldehyde and salvianolic acid b in 6 hours,and its ph was determined at 25℃for 24 hours.

方法采用高效液相色谱法梯度洗脱测定配伍液中6 h内丹参素、原儿茶醛、丹酚酸b的含量。并在25℃时24 h测定配伍液的ph值。

Methods:The RP-HPLC method was applied and protocatechuic aldehyde was used as the internal standard substance.

采用RP-HPLC法，以原儿茶醛为内参照物，色谱柱：Luna C_(18)柱(250mm×4.6mm，5μm)，流动相：0.05%磷酸水溶液-乙腈，梯度洗脱程序为：0min时A-B(100∶0)，80min时A-B(67∶33)，85min时A-B(60∶40)，100min时A-B(60∶40)，流速：1.0mL·min~(-1)，检测波长：280nm。

Objective To determine simultaneously the contents of ephedrine and pseudoephedrine in ephedra herb and its compound preparations by high performance liquid chromatography.

目的 用反相高效液相色谱梯度洗脱法测定麻黄及其复方制剂中麻黄类生物碱的含量。

A ramose input an d multi-output dynamic recurrent neural , network model RDRNN is presented in the , paper.

提出了一种多输入多输出分支动态递归神经网络模型，利用梯度下降法推导了网络权值调整公式。

Fuzzy neural network is used in the three phase adaptive reclosure so that the reclose of permanent faults can be avoided. A multi-input fuzzy neural network is constructed and the algorithm of network is designed. Taking the virtual value of three phase voltage as the input with its scale changed, the network learning can be completed with convergence reached after the process of fuzzification, normalization and perspicuity, so it can disinguish transient faults from permanent faults.

为了避免重合闸重合于永久性故障，将模糊神经网络应用于三相自适应重合闸中，构造了一个多输入的模糊神经网络，设计了网络的算法，将三相端压有效值作为输入，经尺度变换、模糊化、归一化和清晰化，并利用梯度下降法修正误差，使得网络完成学习并最终收敛，在输入故障数据时能根据网络的输出结果准确地判别瞬时性故障与永久性故障。

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。