梯度法

Heterodon specimens as successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205bp of the control region of mtDNA from all of the ten specimens was successfully amplified.

本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚－氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。

These indicates bioactive peptides in Chinese forest frog skins have great potential for the exploitation and utilization. S_(180) cells were inoculated hypodermically at the concentration of 1×10~6 cells per mouse and the bioactive peptides in Chinese forest frog skins were resolved into aseptically distilled water to make injection liquid. After injected continuously 15 days, the inhibition rate of tumor was calculated. The induction of tumor cell apoptosis of active peptides in Chinese forest frog skins could be detected by morphological

将S_(180)胃肉瘤细胞接种至小鼠皮下，选取中国林蛙皮肤生物活性肽不同浓度样品液，连续注射种瘤小鼠15天后，计算抑瘤率；采用透射电镜观察细胞形态学变化、DNA梯度琼脂糖凝胶电泳技术、MTT比色法、流式细胞检测等技术观察中国林蛙皮肤生物活性肽对K562肿瘤细胞凋亡过程的诱导。

Methods The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous Percoll density gradient centrif...

分别采用差速贴壁法和Percoll非连续密度梯度离心法进行新西兰兔肌卫星细胞的原代培养并进行纯化。

Methods: the present study improved isolation of LDL, undertook one-step of density gradient centrifugation, generate E-LDL by treatment LDL with multiple enzymes, direct fluorescent label CRP and LDL, and investigate whether CRP play as intermedial mediator in atherosclerosis. Experiment consists of 8 groups: control group, N-LDL group, ox-LDL group, E-LDL group, CRP+N-LDL group, CRP+ox-LDL group, CRP+E-LDL group.

本研究参考文献改进LDL提取方法，通过一次性密度梯度超速离心，分离LDL，并且通过复合酶处理以及铜离子氧化法分别生成E-LDL和ox-LDL，并直接对CRP及LDL进行荧光标记，通过与内皮细胞共孵育，利用荧光显微镜检测CRP及LDL与内皮细胞结合，实验分为8组：空白对照组、CRP组、N-LDL组、ox-LDL组、E-LDL组、CRP+N-LDL组、CRP+ox-LDL组、CRP+E-LDL组。

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC；用光镜形态学、Ⅷ因子和CDl4免疫细胞化学染色及RECA.1单抗间接免疫荧光法及SEM鉴定SEC；采用TEM及AnnexinV联合PI双染的方法检测SEC的凋亡。

Second, we put lens in front of the right eye of the subjects, the degree of lens were increased laddered by 0.50D (range+1. 00～+4. 00D,-1. 00～-4. 00D), induced simply myopia and hyperopia anisometropia.

第二阶段用插片法在受试者右眼前放置正负球镜，以0.50D为梯度逐级增大，范围+1.00D～+4.00D、-1.00D～-4.00D，分别诱导单纯近视和远视性屈光参差。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

The avenge recovery rates of icariin and naringin were 100.04% and 100.23%, respectively.

用HPLC法梯度洗脱能将淫羊藿苷和柚皮苷较好地分离检测，提高时效，减少误差。

Methods: Rat MSCs were isolated with percoll and nonadherent cells were discarded 48 hours later. The shape was observed and CD34, CD44, CD45, CD166 were detected with Flow cytometry analysis. The growth curve of MSCs in different concentration of fetal bovine serum was observed by MTT colorimetry. The growth curve and attachment efficiency of MSCs of passage 1 (P1), P3, P5 and P10 were also observed.

采用密度梯度离心法结合贴壁分离法分离培养大鼠骨髓MSCs，观察其形态，流式细胞仪检测细胞表面标记，MTT法观察不同浓度的胎牛血清对MSCs生长曲线的影响及不同代MSCs的生长曲线和细胞贴壁率。

The results show that the learning algorithm of FNN based on PSO is superior to the BP algorithm observably.

BP算法实质上是一种梯度下降法，算法性能依赖于初始条件，学习过程易于陷入局部极小。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。