梯度法

The recombinant proteins form inclusionbody in cytoplasm. Dissolve the inclusionbody with urea and refold it with metal affinity chromatography. The refold recombinant protein has certain antibacterial activity.

对表达产物进行复性，结果显示无论是融合表达的VHb-Fetidin还是非融合表达的Fetidin都无法用稀释法和尿素浓度梯度透析法成功复性，只有VHb-Fetidin可以通过金属亲和层析柱成功复性。

So it can be avoided that the computation accuracy is lowered and the calculation result is hard to be convergent for the odd state of transformation matrix.The computation accuracy of wavelet Ritz method and wavelet Galerkin method has been improved so the special'microscopic'property could be fully expressed. An effective method is offered also to solve the problem of high stress gradient and some other odd state problems in engineering.

从而避免了由于转换矩阵奇异而造成精度下降且计算结果不易收敛的问题，提升了小波Ritz法和小波Galerkin法的求解精度，使小波分析的"显微"特性得以充分发挥，并为应力大梯度问题和工程奇异问题的有效求解提供了强有力的计算手段。

A hybrid method based on differential evolution and subgradient optimization is used to solve the problem. It is found that the proposed method can find solutions better than the de facto commercial software LINGO.

在采用一种混合微分演化及次梯度优化法来解决此问题之后，实验结果发现这种混合法比业界公认的商业软体LINGO能够找到更好的答案。

In order to make the new recursive method convergence fast, the RLS-like forgetting factor is introduced to construct the new data Hankel matrices. In the framework of subspace methods, the gradient type subspace tracking method is used to implement the update of the state subspace, which results the unbiased estimate. Different from traditional offline method, the system matrices are recursively computed using RLS method. The convergence analysis of the proposed method is given and finally, the efficiency of this method is illustrated with a simulation example.A new closed-loop identification method is proposed to implement consistency estimation of closed-loop subspace identification.

引入RLS-like遗忘因子机制构造新的数据矩阵，更好地跟踪系统时变信息，提高递推算法的收敛速度；在子空间辨识框架内，采用梯度型子空间跟踪算法实现对状态子空间的更新，避免了子空间近似带来的估计有偏性；与传统的离线算法不同，在得到系统状态子空间后，基于递推最小二乘方法提出新的算法，实现对系统矩阵的递推估计；并给出递推子空间辨识算法的收敛性分析；最后通过仿真实例验证算法的有效性。

Aluminum oxide;Nickel aluminide;Functional gradient composite material;Oxidation resistance;Hydrothermal method;Hydrothermal anodic oxidation

中文关键字氧化铝；镍铝介金属；功能梯度复合材料；抗氧化性；水热法；水热阳极氧化法

BiConjugate Gradients Stabilized method is applied for solving large linear equation set. That the calculation results of PC modeling program match well with that of parallel algorithm shows high reliability of the latter. The parallel algorithm has established the basis for the inversion 3D Borehole-Surface electromagnetic data and also provided the tech support for the utilization of the program.

在求解大型线性方程组时，采用的是稳定的双共轭梯度算法，单机正演程序与并行正演程序的计算结果有着很好的一致性，这说明了并行算法的正确性，为进一步开发井地电法三维反演并行程序奠定了基础，也为井地电法三维正、反演程序实用化提供了技术支持。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

A method for the separation and determination of Pueraria lobata isoflavonoid extract by reversed-phase high performance liquid chromatography was proposed.

采用反相高效液相色谱法，在C1 8柱上以甲醇水为流动相进行梯度洗脱，在 0～ 1 0min内将葛根黄酮提取物进行分离，并在 2 5 0nm检测，采用标准加入法对实际样品中的葛根素含量进行了分析。

Methods first , to acquire the highly pured glomeruli by three steps grade filtrated net ; next , to culture the microvascular endothelial cell in vitro with the transplant methods of isolated glomerali.

方法采用三步梯度筛网法，先获得较高纯度的肾血管球，再应用肾血管球外植法体外培养肾微血管内皮细胞。

Methods After enzymatic digestions of rat testis,the suspension passed the BSA uncontinuous gradient medium in a gravity unit by velocity sedimentation.

方法酶消化法制备大鼠睾丸组织单细胞悬液，采用牛血清白蛋白不连续密度梯度沉降法分离Sertoli细胞和精原细胞。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。