梭子蟹属

In addition, the phylogenetic relationship among several genera of Portunidea was discussed using Eriochier sinensis as the out-group.

另外，以中华绒螯蟹为外群探讨了梭子蟹科几个属种的系统进化关系。

All homologous partial sequences of COI and 16S in Portunidae were collected from GenBank and aligned with the homologous sequences of P.trituberculatus. The NJ tree and MP tree were constructed using Kimura 2-parameter model. Homologous sequences (429 bp) of sampled specimen and genus Charybdis were aligned.

结合GenBank中收集的梭子蟹科COI，16 S rRNA两个基因所有同源序列信息，使用Kimura双参数模型采用邻接法、最大简约法构建梭子蟹科分子系统发育树；将样品蟹测得的COI基因序列和已知属的其他蟹同源序列(429 bp)进行比对分析。

The molecular phylogenetic trees were constructed by the NJ method using software MEGA4.0, and Portunus was clustered with Callinectes first, then they were clustered with Charybdis, and finally with Eriochier sinensis. The results are consistent with classical taxonomy.

用MEGA4.0软件中的NJ法构建了系统进化树，基于16S rRNA和COⅠ2种片段的聚类结果均显示梭子蟹属与美青蟹属亲缘关系最近，先聚在一起，然后再与蟳属聚在一起，最后才与外群中华绒螯蟹聚在一起，这一结果与传统分类基本一致。

Portunus trituberculatus is a famous aquatic variety in Ningbo city and melanin is often found in storage period. It is a big problem to prohibit melanin phenomenon and prolong storage period of Portunus trituberculatus.

三疣梭子蟹属宁波名特优水产养殖品种，但三疣梭子蟹在保藏加工中容易发生褐变，哪种包装方式可抑制梭子蟹褐变并有效延长产品保鲜期，是一个值得探讨的问题。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。