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To harmful microflora white part of birch bark is obliged precisely to betulin.

稳定有害菌群白桦树皮部分恰恰有义务为桦木醇。

The microwave-assisted extraction of betulin from Betula platyphylla Suk. bark was optimized using uniform design.

利用均匀设计法优化了微波辅助提取白桦树皮中桦木醇的工艺。

The results showed a betulin yield of 25.43%, a purity of 59.19% was achieved from 1g powder of B. platyphylla bark under the extraction temperature of 72℃, microwave power 67W, and extraction time 100s.

试验结果表明,1g白桦树皮粉末,在提取温度72℃、微波功率67W,提取时间100s的条件下,微波辅助提取的桦木醇收率达到25.43%,纯度达到59.19%。

Abstract〕 Objective To study 23-hydroxyl betulinic acid induced apoptosis and growth inhibition of B16 melanoma cell in vivo and in vitro.

摘要〕 目的研究23-羟基桦木酸对体内、外B16黑色素瘤细胞生长的抑制及诱导凋亡作用。

And a birch bark compound, betulinic acid, is being tested as a treatment for melanoma and other serious diseases.

一、桦树皮大院桦木酸,正测试作为治疗黑素瘤和其他严重疾病。

Key words〕 23-hydroxyl betulinic acid;B16 melanoma cell;apoptosis

关键词〕 23-羟基桦木酸;黑色素瘤细胞;细胞凋亡

Methods MTT assay,electronic microscopy,flow cytometry and tumor volume assay were used to examine the effect of 23-hydroxyl betulinic acid on B16 melanoma cell line.

应用MTT分析,电镜观察,流式细胞仪,肿瘤体积大小测定等方法,对23-羟基桦木酸诱导黑色素瘤细胞进行检测和观察。

The substance, betulinic acid, had weak actiity against HI.

这个物质就是桦木酸,有着些微的抗HI活性。

Een though researchers understood that PA-457 seemed to hae actiity against all strains of HI, they needed to find out how the betulinic acid deriatie worked against the irus on the molecular leel.

研究人员了解到PA-457似乎可对抗所有品系的HI后,还需要知道桦木酸的衍生物在分子层次上怎样对付病毒。

Results 30~80μg/ml 23-hydroxyl betulinic acid in vitro and 300mg/kg~600mg/kg P.O.in vitro markedly inhibited the growth of B16 cells at a dose-dependent manner with a great number of apoptotic cells seen.

结果 23-羟基桦木酸体外30~80μg/ml处理,体内300mg/kg~600mg/kg灌胃时,对B16细胞有显著的生长抑制作用,有一定的剂量依赖关系并出现大量的凋亡细胞,同时体内肿瘤细胞被阻滞在G0~G1期,DNA合成受阻,肿瘤体积缩小。

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