桑葚

Mulberry contained84.71%moisture,0.36%crude protein,9.61%invert sugar,0.66%ash content ,0.91%coarse fibre,and some malic acid,vitamin B 1 ,vitamin B 2 ,lactoflavin,carotin etc.

桑葚含水分84.71%、粗蛋白0.36%、转化糖9.16%、灰分0.66%、粗纤维0.91%、苹果酸、维生素B1、维生素B2、核黄素、胡萝卜素等。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Rabbit(Oryctolagus cuniculus domestica); mRNA differential display ; Morula stage embryo ; Zygotic gene

兔； mRNA差异显示技术；桑葚胚；合子型基因

Germ potential is established after conception in a totipotent zygote and retained subsequently during development in blastomers of morula , cells form the inner cell mass of blastocyst , epiblast and population of primordial germ cells .

种子潜能的确定是全能的受精卵形成之后，并且随后在桑葚胚，胚泡内层细胞，外胚层和原生殖细胞的发育过程中保留。

One afternoon after school, having nothing better to do as my parents were busy doing farmwork in the fields, I decided to go mulberry-picking at the riverside with several playmates.

那发生在我上小学二年级，一天下午放学后，大人门都在地里忙着干活，我们小孩子放学没事干，所以我们几个孩子决定去河边旁边的桑树那块摘桑葚

It transforms the zygote into a cluster of cells called morula which, seen through a microscope, resembles a mulberry.

卵裂把受精卵转换成称为桑葚胚的细胞团，它在显微镜下看起来象桑葚果。

With the prolonging of treatment with 6-DMAP,the developmental ability of parthenogenetic embryos decreased.

随着6-DMAP处理时间加长，孤雌胚的发育能力降低。6-DMAP处理1小时的桑葚胚／囊胚的发育率(72％)远远高于6-DMAP处理6小时的桑葚胚／囊胚的发育率(28％)。

Go to pick mulberries for the second time!

二次去摘桑葚，途中遇一小狗儿，亦步亦趋，非要跟在我们后面走，我们走它也走，我们停它也停，摘桑葚的时候就坐在地上傻傻地看，扔给它吃它嗅嗅不吃又抬起头来看你，真惹人喜爱！

Mulberries, pelted each other with them, giggling, laughing; I can still see

袋子里装满了干桑葚和胡桃，吃着桑葚，打闹着，笑着。。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。