根的生长

And then the two different turfgrass seed germination tests conducted, results show that: 0.1 percent concentration of hydrogen peroxide concentration of 0.01% of the "Bi-care" and the concentration of 0.29 percent for the "God LU Hong Lu," The three kinds of reagents soaking treatment can significantly improve the wild Pennisetum and Eragrostis seed germination and growth of 0.10 percent by the concentration of the soaking H2O2 solution, then 0.29% concentration of the "God LU Hong Lu" after soaking solution, wild Pennisetum Eragrostis seeds and seed germination energy, germination percentage, germination index, root length and were significantly higher than control to deal with the seeds, but also to varying degrees higher than the other chemical reagents used to deal with the seeds, rapid germination and tidy.

然后对两种草坪草不同的种子处理进行了发芽试验，结果表明：浓度为0.1%的过氧化氢、浓度为0.01%的&碧护&和浓度为0.29%的&鲁虹神露&这三种试剂浸种处理能显著提高野生狼尾草和大画眉草种子的萌发和生长，经浓度为0.10%的H2O2溶液浸种，再经浓度为0.29%的&鲁虹神露&溶液浸种处理后，野生狼尾草种子和大画眉草种子的发芽势、发芽率、发芽指数、根长和芽长均显著高于空白对照处理的种子，也不同程度的高于用其他化学试剂处理的种子，发芽快而整齐。

The inhibition substances in Sorbus pohuashanensisHedl fruit not only restrained the seeds of wheat, cabbage from germinating but also restrained height and root growth. After the seeds of wheat, chinese cabbage had been soaked in the water extraction of pulp, seed and decoated seed for 8h, the seed germination rates were 92% and 84%, 89% and 65%, 94% and 78%, lower than the control, especially the water extract of decoated seed; and the root length and seedlings height were lower than the control, especially the water extract of pulp, and decreased in sequence of soaking time from short to long. So the simplest and effective method to eliminate pulp soluble inhibiting substances is to wash fruits by flowing tap water soon after collection.

在几种浸泡时间中，浸泡8小时的去皮种子浸泡液对白菜、小麦种子发芽率的抑制作用最为明显，其发芽率分别为65%、89%，但随着浸泡时间的延长而降低；浸泡8h的果肉浸泡液对白菜和小麦幼苗高生长和根伸长的抑制作用最为明显，种子和去皮种子浸泡液(8h)的抑制作用对小麦和白菜的抑制作用次之，随着浸泡时间的延长，果肉浸泡液的抑制作用逐渐减弱，因而采收下来的果实及时净种是清除果肉水溶性抑制物质简单有效的方法。

Soaking seed of rice and wheat with zymolytic liquid of Trichoderma harziaiarum, Trichoderma DWC and Trichoderma viride, comparing with germinate ratio, germinate force, root length, bud length and ratio of root to bud, the results show that 50 times dilute zymolytic liquid of Trichoderma harziaiarum has a relatively notable promote role for germinate of seed .The effect of Trichoderma viride to germinate of seed is not evidence. Trichoderma DWC for seed sprout has certain inhibition.

据此，采用哈茨木霉作为出发菌株，通过离子束诱变，从大菌落株中筛选出促进生长的高产突变株——h-13菌株，通过对用该菌株发酵液处理后的水稻及小麦的种子的各项生理生化指标的测定，发现该菌株比其它菌株更能促进水稻和小麦的活力和发芽率，三天时小麦的总根长为3.03cm，比对照的1.42cm的两倍还多，并且能够明显提高种子发芽后的淀粉酶和蛋白酶的活力，其活力都是对照的两倍左右。

In this study, 105 TDFs were in silico mapped in the rice high-density linkage map. Nineteen TDFs were mapped to the quantitative trait loci regions for root growth under water-limited conditions in, at least, two of three rice populations derived from three crosses with the same parent of Azucena (Bala×Azucena, IR64×Azucena and IR1552×Azucena). Four of 19 genes (T37, L16, T17 and T7) were mapped based a RIL population of IR1552×Azucena by southern blot analysis. Five genes encode putative or hypothetical protein. Other 14 genes were similar with known genes in databases including expansin (OsEXP2), late embryogenesis-abundant gene , an SR-related protein essential for spliceosome assembly (SART1), autophagocytosis protein , bHLH protein, fruit-ripening protein similar to ASR, nickel-binding protein 2A, DNA-binding protein, pyruvate dehydrogenase kinase , stomatin-like protein, SR1 induced by sucrose starvation, vacuolar protein sorting protein (VSP33a), gibberellin action negative regulator and retroelement.

根据核苷酸序列将105个基因电子定位到水稻的高密度连锁图谱上，其中19个差异表达基因定位在Bala×Azucena、IR64×Azucena和IR1552×Azucena中至少两个群体共同的与根生长相关的QTLs区间，并用Southern杂交将其中的4个定位到IR1552×Azucena群体遗传连锁图谱相应的位点上。19个基因中的5个编码推断的未知功能蛋白质，其余14个编码已知功能蛋白，分别为膨胀素（Os-EXP2）、胚胎后期丰富蛋白、剪接体安装必需的SR相关蛋白（SART1）、自吞噬蛋白、碱性螺旋-环-螺旋转录因子、与ASR相似的果实成熟蛋白、镍结合蛋白、DNA结合蛋白、丙酮酸脱氢酶激酶、stomatins类蛋白、蔗糖调节蛋白SR1、液泡蛋白分类蛋白（VSP33a）、GA负调节因子、逆转座元件。

The details are summarized as follows: 1.γ-irradiation is extended to control the morphology of microcrystals. In our experiments, copper acetate aqueous solution, a simple reaction system, is used at room temperature to prepare pure hexapod-shaped Cu〓O microcrystals, in which a buffer pair is in-situ formed to adjust the acidity of the reaction solution. Some important information on intermediate is obtained by changing the reaction parameter, which favors to reveal the inherent growth habit of Cu〓O.

发展了γ-射线辐照合成法对微晶的形貌的控制，在常温常压下从一个简单的反应体系——醋酸铜水溶液出发，通过辐照过程中原位产生的醋酸-醋酸根缓冲对的自发调节作用，控制反应体系的酸度从而合成得到了纯相的六足状氧化亚铜微晶；并通过改变反应参数，获得了中间体的信息，从而为揭示氧化亚铜的固有生长习性提供了条件。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。