根瘤菌

The rhizobia isolated from Indigofera, Kummerowia and Astragalus in Shaanxi, Gansu and Ningxia were firstly and systematically studied.

首次对国内外未曾作过分类研究的木蓝属和鸡眼草属的根瘤菌以及陕甘宁地区的黄芪属根瘤菌进行全面、深入、系统地研究。

Based on the investigations of rhizobia resource of Shaanxi, Gansu and Ningxia, the research applied polyphaic taxonomy to study the rhizobia isolated from Astragalus, Indigofera, Kummerowia and so on in the different levels.

本研究在陕甘宁地区豆科植物根瘤菌资源调查的基础上，利用多相分类技术，从不同层次水平系统地研究了分离自黄芪、木蓝、鸡眼草等寄主的根瘤菌。

Prain, Trifolium repens Lam, Aeachynomene Mica L, Indigofera bungeana Steud.The same strains used in numerical taxonomy were analysed by BOXAIR-PCR fingerprinting. All tested strains were clustered into 47 groups at the similarity level of 50%. Compared with the result of numerical taxonomy, strains belonged to one phenon in numerical taxonomy always distributed into several BOXAIR-PCR groups, most of reference strains come from one recognized rhizobial species were not grouped together.

B(来源：dd9dAB94C论文网www.abclunwen.com)OXAIR-PCR指纹图谱的聚类结果显示：全部供试菌株在50％的相似性水平上分为47群，与数值分类的结果相比较，其分群的结果更分散，在数值分类80％相似性水平上位于同一表观群的供试未知菌株大多分布于几个综合的BOXAIR-PCR群内；另外在数值分类中来自同一个种的已知根瘤菌参比菌株几乎都聚在同一个表观群，而在BOXAIR-PCR分析中，已知参比根瘤菌的同一个种内的大部分菌株都单独成群。

Further study of the interaction between NodD and naringenin by FRET technology is underway.

通过对naringenin分子上六个不同位点，不同性质，长度连接链以及母体和荧光团之间两种连接方式的11个荧光标记物的合成以及生物活性评价，我们找到了一个荧光标记物，它保留了野生型naringenin约20％的活性，并将其用于根瘤菌固氮过程的研究，发现该荧光标记物能够顺利进入根瘤菌活细胞中，为下一步通过FRET技术研究naringenin和NodD蛋白之间的相互作用打下了基础。

In order to discover acid-tolerant Rhizobium meliloti that can nodulate alfalfa high-effectively on acidic soil, researches were explored to isolate rhizobia from alfalfa nodules on acidic soil (pH5.2), methods based on the thesis of adaptive acid-tolerance response of the Rhizobia were adopted, six strains were isolated, which could grow on solid medium with pH4.8 steadily. In addition, the nodulation characteristics, growth curves and mean generation time under different acidity of the strains were examined.

为获得能够使紫花苜蓿在酸性土壤中高效结瘤的耐酸根瘤菌，该研究从酸性土（pH5.2）上生长的紫花苜蓿根瘤中分离到若干菌株，利用根瘤菌对酸的适应性耐受反应筛选到六株能够在pH4.8的固体培养基上稳定生长的菌株，并检测了这六株菌的结瘤特征、不同酸度下的生长曲线和平均代时。

One could nodulate the soybean cultivars tested and showed wider host range, the other only nodulate limited soybean cultivars. Plasmid could not be detected from twenty-three slow-growing strains and twenty-nine fast-growing strains were found containing 1-4 plasmids by Echardts method.

质粒快速检测结果表明：慢生型大豆根瘤菌均不含有质粒，快生型大豆根瘤菌均有1-4个质粒，质粒类型与宿主亲和性也有一定的相关性。

Sinorhizobium fredii 042BS was isolated from root nodules of alfalfa from Xinjiang Region. Nodulation experiments showed that both soybean and alfalfa were nodulated by 042BS effectively.

费氏中华根瘤菌042BS分离自新疆的苜蓿根瘤，交叉结瘤试验发现它既可以在苜蓿上又能在大豆上结瘤固氮。16S rDNA PCR-RFLP分析表明，042BS与费氏中华根瘤菌模式菌株USDA205的4种限制性酶切图谱完全一致。

Diversity and phylogeny of rhizobia isolated from root nodules of Sesbania cannabina in Jinshajiang arid river valley;2. To study the diversity and phylogeny of rhizobia from Sesbania cannabina in JinShaJiang arid river vally in Sichuan,48 rhizobia was isolated,nodulated,and studied by numerical taxonomy,BOXAIR-PCR,16S rDNA PCR-RFLP,sequences analysis of 16S rDNA and GSⅡ.

从金沙江干热河谷区田菁根瘤中分离得到48株根瘤菌，在对其进行回接实验后，采用数值分类、BOXAIR-PCR、16S rDNAPCR-RFLP、16S rDNA和GSⅡ序列分析方法对这些菌株进行研究，以揭示在金沙江干热河谷区这一特殊地理环境下田菁根瘤菌的多样性和系统发育地位。

In order to investigate the electrotransformation condition of Sinorhizobium meliloti variable curve was devised by different cell cycles time,resuscitative buffer,electrotransfection buffer,pulsed electric field and resistance.

为了研究苜蓿中华根瘤菌电转化的条件，通过对不同的细胞生长周期、复苏缓冲液、电转缓冲液、电场强度和电阻对电转率影响程度来作出变化曲线，以确定电转化的最优条件，从而提高苜蓿中华根瘤菌电转化率。

102 phenotypic features of 16 strains isolated from Sesbania ,growing in dry hot valley region of panzhihua,were analyzed.13 reference strains were included for comparison.The result showed that there were great diversities among strains in carbon and nitrogen nutrient utilization,resistance to antibiotics,endurance to salt.

选用分离自攀枝花干热河谷地区的田菁根瘤菌未知菌16株和13株参比菌株，进行了102项表型性状测定，结果表明：供试根瘤菌株在碳氮源利用、抗生素敏感性、抗逆性等方面存在着差异。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。