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Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.

本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒,编码NPTⅡ基因和GUS基因)转化烟草沙姆逊,获得了具有卡那霉素抗性烟草发状根,并筛选出良好的单克隆株系,该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长),通过根尖压片分析,烟草发状根不具备典型的根冠;发状根中烟碱含量1.61%稍高于与天然栽培品种母体根的含量(1.5%左右),发状根向培养液中释放烟碱,释放量最多时占总烟碱量的81.1%;在无激素的培养基上获得了再生植株。

Elicitation has become one of the most effective methods for enhancing secondary metabolite production in hairy root cultures.

目前在毛状根中已有的研究结果表明诱导也成为了提高毛状根中次生代谢产物产量的有效方法。

Rootstock ± vertical, 5-12 mm in diam., not tuberculate, with thickened roots along rootstock. Stem 16-50 cm tall, erect, not rooting at nodes, with 0.2-1.3 mm retrorse to patent nonglandular trichomes and rarely 0.7-0.8 mm patent glandular trichomes.

根茎多少垂直,直径5-12毫米,不具瘤,根沿着根茎加粗。16-50厘米高的茎,直立,并非根在节上,具0.2-1.3毫米对开展非腺状毛状体和很少开展腺毛0.7-0.8毫米。

Methods:Fifty paired embalmed cadaveric humeri (twenty-five pairs:fourteen from male donors and eleven from female donors) were scanned in medial-lateral and anterior-posterior position according to the humeral retroversion by CT. Images of the humeri in the transverse planes at the lowest border of neck,20mm and 40mm distal of LBN(LBN-20、LBN-40), isthmus, head-neck anterior-posteriorwere obtained. Sixty-one extracortical and intracortical parameters were measured exactly by image analytic computer software that included offset, head position, head-shaft angle, head to tuberosity height, head thickness, curvature radius, articular surface arc, neck diameter, isthmus position, proximal and distal border of isthmus, maximum coronal and sagittal diameter of medullary canal and thickness of cortical bone in four planes, including LBN, LBN-20,LBN-40 and isthmus.

50根成对防腐肱骨(男14对,女11对)按肱骨头扭转角置于冠状位和矢状位,行肱骨全长,头颈矢状面,解剖颈下缘及其下20mm、40mm,髓腔狭窄部四平面CT扫描,由CT软件测量冠、矢状位髓腔内外参数共61项,包括头心—干轴距,头位置,头干角,头—结节高度差,头厚度,头半径,关节面张角,解剖颈直径,髓腔狭窄部位置,解剖颈下缘及其下20mm、40mm和狭窄部四个平面髓腔的最大冠、矢状径,皮质骨厚度等。

In the soil introduced with T. citrinoviride agent, the dominate Trichoderma species at rhizosphere were T. hamatum and T. citrinoviride while the dominate Trichoderma species at non-rhizosphere was T. hamatum.

在对照茄子土壤中,根际与非根际土壤的优势木霉种群为钩状木霉;在桔绿木霉处理土壤中,根际木霉优势种为钩状木霉和桔绿木霉,非根际土壤中木霉优势种为钩状木霉。

Methods: The distal 1/3 of eighty humeri was scanned by 16 layer spiral CT. The morphologic features of the intramedullary bony prominence above the olecranon fossa were observed and described on coronal, sagittal and transverse section after three-dimensional reconstruction. Then the distal 1/3 of twenty humeri was splitted vertically along the coronal midline for observing the intramedullary bony crista.

应用16层CT对80根肱骨远段1/3进行扫描,三维重建成像后,从冠状面、矢状面和横截面观察并描述鹰嘴窝上髓腔内骨性突起的形态特点,然后将20根肱骨远段1/3在冠状面上从中线纵行剖开,对髓腔内骨嵴行直观的观察。

RESULTS: Totally 57 rats enrolled in this study, all animals completed Hot pain allergic symptom could not be observed on rats in experimental group during the whole experimental process, but continuously appeared in discharge percentage of nerve fibers at the central end of experimental rat sciatic nerve was remarkably lower than that of the control group 6.79%Sympathetic postganglion nerve fiber budding was not obvious in the slices of entire L4-5 dorsal root neuroganglion of injury side in 6 experimental rats,and basket-shaped cell could not be observed, but sympathetic postganglion nerve fiber budding was obvious in 2 rats in control groups with altogether 41 basket-shaped cells observed.

电生理实验:实验组大鼠坐骨神经中枢端的神经纤维自发放电百分数显著低于对照组(6.79%,46 7%)。③背根神经节切片荧光染色结果:实验组6只大鼠损伤侧L4-5的全部背根神经节切片中看不到明显的交感节后神经纤维芽生,没找到篮状细胞,而在对照组2只大鼠损伤侧L4-5的全部背根神经节切片中可看到明显的交感节后神经纤维芽生,共找到篮状细胞41只。

Among three auxins , IAA at low concentration was the best for growth of Type Ⅰ hairy root, while 1mg/l IBA was the most suitable for growth of Type Ⅱ hairy root.

在发状根的继代培养基中添加外源生长激素(IAA、IBA、NAA),低浓度的IAA更适合于Ⅰ型发状根的增殖培养,而在促进Ⅱ型发状根的增殖和侧根形成方面,1mg/lIBA的作用最强。

A broad expanse of the river was turned to blood;1 in the middle distance the red hue brightened into gold, through which a solitary log came floating black and conspicuous;2 in one place a long, slanting mark lay sparkling upon the water; in another the surface was broken by boiling, tumbling rings, that were as many-tinted as an opal; where the ruddy flush was faintest, was a smooth spot that was covered with graceful circles and radiating lines, ever so delicately traced; the shore on our left was densely wooded, and the somber shadow that fell from this forest was broken in one place by a long, ruffled trail that shone like silver; and high above the forest wall a clean-stemmed dead tree waved a single leafy bough that glowed like a flame in the unobstructed splendor that was flowing from the sun.

不远处红光闪成金黄;孤零零的一根原木在河中漂浮而来,呈一线醒目的黑色;河的一处,一条长长的斜影横落在水面上,闪闪烁烁;另一处,水面被翻滚的水波打碎,仿佛蛋白石放射的斑斓色彩;红彩最淡处是一片平静的水面,覆盖着优雅的环形和放射状的波纹,细腻如绘;河左岸是一片浓密的森林,一条长长的崎岖小径划破树林的一片荫翳,闪闪如银;一株光秃秃的枯树高耸于密林之上,仅剩的一杆青枝随风摇曳,像火焰闪耀在阳光洒下的一览无遗的壮美之中。

Results In healthy subjects, L2-L5 nerves were displayed clearly by MRN, with a round or ellipse ganglion of 3-6 mm in diameter, and smooth stick-like nerve root and trunk, 2-5 mm in diameter. The signal intensity was higher in ganglion than nerve root or trunk. 40% of nerve root sheath was surrounded by small amount of cerebrospinal fluid, Femoral nerve was displayed in all subjects, while only 83.3% of obturator nerve and 43.3% of lumbosacral trunk were manifested. Lamellar blurred hyper-signal intensity was seen around 80.9% of lumbar plexus in GBS, but none in CIDP.

结果 正常组MRN可清晰显示腰2~5神经的走行,神经节呈圆或椭圆形膨大,大小约为3~6mm,神经根及干呈条状、边缘光滑,大小约为2~5mm、神经节信号高于神经根和干,40%的神经根鞘有少量脑脊液,股神经、闭孔神经、腰骶干的显示率分别为100%、83.3%、43.3%。80.9%的GBS腰丛神经周围有片状模糊高信号,神经根和节大小和信号无明显改变,25%神经干增粗,50%的神经干信号增高,边缘模糊。

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