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In order to select high nitrogen-fixing azotobacter strains, nitrogenase activity of 13 strains separated in the rhizosphere in Gansu non-leguminous crop fields were measured by acetylene reduction method. Also the fertilization effect of the strains on spring wheat was investigated via pot experiment.

为挑选具有高固氮能力的自生固氮菌菌株,用乙炔还原法对甘肃省春小麦和玉米等非豆科作物根际土壤中分离所得的13株菌进行固氮酶活性测定,并通过盆栽试验对其进行春小麦肥效试验。

The results showed that Hyphae, conidiophore, conidium and chlamydospore of biocontrol strains ZK7 and IPC were found in the rhizoplane, Hyphae of ZK7 and IPC penetrated epidermal cells to deep cortical cells but not in phloem tissue and xylem vessels, biocontrol strains ZK7 and IPC also crossed and even grew within epidermal and conical cell walls without any visible alterations of the plant cell.

生防菌ZK7和IPC菌株能以菌丝方式稳定定殖于烟草根表的根冠区、伸长区、根毛区、成熟区等不同部位,而在根内的定殖仅局限于根表皮层较深的细胞组织,不能侵入烟草根部的韧皮部和木质部组织。

A biocontrol strain, XF-1, was identified as Bacillus subtilis based on morphology, physiology, biochemistry and biotechnology, which could control clubroot of cruciferous crops effectively.

依据传统形态学、生理生化特征及16S rRNA基因序列,将一株对十字花科根肿病有很好防治效果的菌株XF-1鉴定为枯草芽孢杆菌。

With the growth-promoting bacteria HRO-C48 in plant rhizosphere as tested strains, the growth-promoting effect of chitosan on tomato and the influence of its combination with HRO-C48 on the biocontrol potential of the strain, including its induced resistance effect on grey mould in tomato and its biocontrol effect on damping-off of cucumber were determined in greenhouse.

以植物根际促生细菌HRO-C48为供试菌株,在温室条件下测定壳聚糖对番茄的促生作用以及HRO-C48与壳聚糖组合对该菌株生防潜能的影响,包括对番茄灰霉病的诱导抗病作用、对黄瓜猝倒病的生物防治效果。

Antagonistic strains of rhizobacteria against the fungi of Rhizoctonia solani, the causative agent of the damping-off disease of mungbean, were selected on potato dextrose agar, tryptic soy agar and malt yeast glucose agar with 62 rhizobacteria isolated from the rhizosphere of mungbean respectively.

采用对峙法分别在马铃薯葡萄糖琼脂,胰酶大豆琼脂和麦芽酵母葡萄糖琼脂3种培养基上测定了从绿豆根际分离的62株细菌对于绿豆立枯病菌的拮抗作用,筛选出2株具有较强拮抗作用,即05-33和05-49。

The results indicated that:(1) plant height and tillering number decreased, and root length, root surface area, root volume and rootlet number increased under drought stress; straw mulched on surface soil and pig manure mixed in soil could improve the growth and morphological property of rice root, while straw mixed in soil and pig manure mulching had no significant effects;(2) the contents of chlorophylls and the activities of SOD, POD and CAT decreased significantly under drought stress, and straw mulching on surface soil and pig manure mixed in soil could restrain these decreases.

结果表明:①干旱胁迫下水稻株高降低、分蘖数减少,根系则表现出主根伸长、支根增多、根表面积和根体积增加;秸秆表施和猪粪混施可明显改善水稻的生长和根系形态,而秸秆混施和猪粪表施的改善作用不显著;②干旱胁迫下叶片叶绿素含量显著降低,超氧化物歧化酶、过氧化物酶及过氧化氢酶活性均显著下降,秸秆表施和猪粪混施能够在一定程度上抑制这种下降幅度。

This paper has studied the physiological and biochemical changes during the period of forming strong buds of tree peony, and expect to provide academic basis for extending its view value and economic benefit . The main results are as the following:1 Under the field condition, Characteristics of photosynthesis of tree peony are very regular. From before blooming to the prophase of abducting buds that after blooming ,that is April,May and June,the net photosynthetic rate of tree peony is higher than the other period .It indicates that this period is very important for accumulating photosynthetic products of tree peony . So, if we want to get quality flower of tree peony ,it is very important to strengthen the management of tree peony cultivation and to ensure provide the fertilizer and water.2 From the middle ten days of June, with the time elapsing and with the environment conditions worsening, the characteristics of photosynthesis of tree peony decline deeply. But it is different among different tree peony breeds. For example , cv. Wu Long Peng Sheng can also has higher net photosynthetic rate, it indicates that cv. Wu Long Peng Sheng is super than the other two tree peony breeds when adapting the environment conditions and cv. Wu Long Peng Sheng has wide prospect to extend .3 The main factors of effecting the characteristics of photosynthesis are stomatal factor and non-stomatal factor ,that is the ability of photosynthesis of leaf cells. Under the environment of high light intensity in midsummer, if overshadow tree peony properly, then it can slow the declining of chlorophyll content, and increase the net photosynthetic rate and benefit for the accumulating of photosynthetic products.4 The content of soluble sugar in the root of tree peony is the highest among different organs during the period of forming strong buds . According to analyse ,we believe that the soluble sugar will compose starch then, and provide the solid base of material for the growth and development next year. It provide us strong academic basis of adopting the method of cultivating root and protecting root under thefield environment conditions .In other words ,it will help to increase the health level of the whole plant of tree peony and will be beneficial to forming quality tree peony flower if we strengthen the management of root. 5 To tree peony, the endogenesis hormone is important for its growth and development .In the prophase ,that is about the blooming of tree peony and the mid-summer ,IAA and ZR contents are higher and GA content is lower and its changes are very little, and the content of ABA is decreasing . This indicates that IAA and ZR act the important promote function for growth and development of tree peony in this period, and the function of GA is not important. Bur in the period of anniversary senescence of tree peony , the content of IAA and ZR begin to decrease ,while the ABA content has a certain rebound .T

本研究对几个牡丹品种壮芽形成过程中的某些生理生化变化做了研究,以期了解牡丹生长发育过程中生理生化的变化规律,探讨大田栽培条件下提高牡丹花质量的栽培措施和管理技术,为提高牡丹的观赏价值和经济效益提供理论依据,结果如下:1 大田栽培条件下,牡丹光合特性在生长发育过程中表现出极强的规律性,牡丹各品种的光合速率从牡丹开花前,到牡丹花谢后的花芽诱导期前期(6月10日之前),也就是在4、5、6月份,各牡丹品种的净光合速率相对与其他时间都维持在比较高的水平,这说明,在这段时间,是牡丹光合产物积累的重要时期,因此,要保证得到高质量的牡丹花,在这段时间加强对牡丹的栽培管理,保证这个时期的肥水供应十分重要。2 从6月中旬后,随时间的推移,环境条件的恶化,牡丹的光合性能剧烈下降,但是不同牡丹品种间仍有差异,比如,乌龙捧盛仍可维持较高的光合水平,这说明了乌龙捧盛这个牡丹品种对于环境的适应性较之其他牡丹品种高,有着广泛的推广前景。3 影响牡丹光合性能的主要因子是气孔因素和非气孔因素即叶肉细胞的光合能力,在盛夏高温高光强的环境条件下,对牡丹进行适当遮荫,能缓解牡丹叶绿素水平的下降,适当提高Pn,有利于光合产物的积累。4 可溶性糖在牡丹各品种的壮芽形成过程中,根中可溶性糖的含量在牡丹各部位中始终维持最高水平,分析认为这些可溶性糖在根系中进一步合成淀粉等储藏性物质,为来年牡丹的生长提供雄厚的物质基础,这为我们在大田栽培条件下对牡丹采取养根护根的栽培措施提供了有力的理论依据,即在大田栽培条件下,加强对牡丹根系的管理,有助于提高牡丹整株的健壮程度,有益于形成高质量的牡丹花。5内源激素对牡丹的生长发育调节作用是十分明显的,在前期,即开花前后和盛夏,IAA和ZR含量维持在比较高的水平,GA含量低且变化不明显,ABA含量逐渐降低,说明了IAA和ZR对牡丹在这段时间内的生长发育起到重要的促进作用,GA的作用不是很明显,ABA可能在高温高光强下有明显的升高,也说明了ABA的确有一些促进作用,但是到了牡丹周年衰老期,IAA和ZR的含量开始下降,ABA含量却有了一定程度的反弹,说明了IAA和ZR的作用开始降低,ABA起到了促进衰老的作用

The mycorrhizal formation,inoculation effectiveness,extraradical fungal biomass and P uptake contribution of six arbuscular mycorrhizal isolates screened from South China,Center China and North China on typical cinnamon soil,brown soil and red soil collected in above three regions were investigated using three-compartment glass-bead culture system associated with maize.

以分离于华北、华中和华南3个生态区及法国引进的丛枝菌根真菌为试验菌株,采用三室根箱培养的方法,研究了它们在华北、华中和华南3种典型土壤—褐土、棕壤和红壤上的菌根形成、接种效应、磷吸收贡献和根外菌物量情况。

In Hubei. After screening, two strains of fungal parasites were gottenwhich had stronger. ability to parasite Meloidogyne eggs. Their parasitic ratio tothe root knot eggs was 78.04% and 70.02% in concave slides and water agar plates,72.37% and 65.65% in pots. The effective hatchings in experimental containers wereonly 5.02% and 8.32%, and there were 100% in controls.

危害的根和根围土壤64份样品,采用3种方法分离,经过筛选测定,获寄生能力较强的真菌2株,室内凹玻片和琼脂平板法测定结果表明,对卵的校正寄生率分别为78.04%和70.02%;室外盆栽测定的寄生率分别为72.37%和65.65%,供试卵有效孵化率为5.02%和8.32%,对照卵为100%。4%土重的棉籽壳真菌制剂能有效控制根结线虫第一代卵量,有效用量的10倍菌剂盆栽测定对植物均没有不良影响。

Under the same eggs inoculation levels,and using such index as root decay,relatively gall index,relatively eggs mass index,shoot height,root length and shoot weight,the pathogenicity for 34 Meloidogyne populations from different areas in 11 provinces in China to the tobacco cultivars K326 and Honghua Dajinyuan were clustered by SAS.

在相同的接种水平下,以烟草品种K326、红花大金元为供试寄主,用SAS软件,对坏死百分率、相对根结指数、相对卵块指数、株高、根长、地上部分重等6个指标进行聚类,分析不同或相似生态条件下,4种常见根结线虫中34个群体的致病性。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

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