根株

Preventing and controlling crown gall disease applied the early biological controls strain K84, itself did not have the pathogenicity, its plasmid may synthesize one kind of bacteriocin Agrocin84, may suppress many pathogenic strains, but did not have pathogenicity to non-pathogenic strain .

防治根癌病应用较早的生防菌株是K84，本身无致病性，它的质粒可以合成一种细菌素Agrocin84，可以抑制很多有致病性的菌株，而对非致病性的菌株则无影响。

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

The dissertation focuses on the breeding of Rhizopus chinensis 12# producing a fibrinolytic enzyme, optimization of solid-...

本论文对产纤溶酶的菌株根霉12#进行了诱变育种，优化了根霉12#产纤溶酶的固态发酵和液态发酵条件，建立了根霉纤溶酶制备分离纯化的方法并进行了优化，研究了根霉纤溶酶的性质。

The plant height is 35 cm and the plant spread is 48 cm. The plume-like leaf cluster is semi-uprush with small tassel. The leaf color is deep green, but the leafstalk is light green and leave numbers per single plant are 10-12. Its fleshy root is of long column shape with rootlet, little fibre and deep green peel. The length is 26-32 cm, the diameter is 8-12 cm and the weight could reach 2-3 kg. The flesh is light green, with delicate and slight pungent taste. And its dry matter content is 7.0%, VC 209 mgkg^(-1), soluble sugar 3.27 % coarse fibre 0.65%. Besides,'Weiluobo No. 1' had high resistance to virus, resistance to downy mildew, stronger resistance to bacterial soft rot than 'Luluobo No.

生长期80d左右，植株生长势旺盛，株高35cm，开展度48cm；叶簇半上冲型，羽状裂叶，小缨，叶深绿色，叶柄淡绿色，单株叶片10~12片；肉质根长圆柱形，小顶小根，须根少，表皮深绿色，仅1/3入土部分为白色，肉质根长26~32cm，横径8~12cm，肉质根质量2~3kg；肉淡绿色，肉质细嫩，干物质含量7.0%，VC209mgkg^(-1)，可溶性糖3.27%，粗纤维0.65%，微辣，品质佳，适于熟食；高抗病毒病，抗霜霉病，对软腐病的抗性优于对照鲁萝卜1号。

In the 50 cm plough layer per m2 area, the total rootstalk weight of L.

结果表明：赖草株高45~120 cm，单生或丛生，具下伸土壤30~50 cm耕层间有横走根群，每平方米内，深50 cm耕层内赖草根茎总重量为1129.3 g，根茎总长度为2783.3 cm，根茎上的新芽589.7个，使土壤严重缺水，肥力下降；土壤水分在13.4%左右时，对出苗影响不大，对生长发育有很大的影响；气温在-6~6℃，根茎萌发出土，高峰期为4月上旬，平均单株结种量为468.4粒；每平方米内有赖草120~160株时，大黄产量损失达15.8%~17.8%；田间每平方米内有赖草1株时，采用人工或机械深耕捡出地下根茎，减轻翌年危害来源。

The results were as follows:The unit-roots characters (weight, length etc.) and 15N absorption decreased progressively downward. 15N absorbed by any unit roots could be transported from root to tiller and from tiller to tiller, but the transporting ratio was different.The pattern was as follows: when 15N was offered to total roots of plant or only to primary roots, it was transported to main stem and then decreassingly to tillers from lower unit to upper one, when 15N was offered to n unit-root, it was transported to n unit tillers, then to n + 2 or n-2 unit tillers , followed by n + 1 or n -1 unit tillers , and finally to other unfeed roots.

各单位根吸收(15)~N后，蘖根之间，蘖蘖之间皆可互相运转，但其运转比例不同，其功能模式是：(15)~N喂全株根或专喂初生根，则先供给主茎，其次是分蘖，而且随蘖位升高而递减；喂n单位根，则先供给n单位本身，其次是n+2或n-2单位的蘖，然后是n+1或n-1单位的蘖，最后是未喂(15)~N的根系。

As compared with the traditional irrigation-traditional fertilization, the following results of the improved traditional irrigation-optimized fertilization were obtained: reduced tiller number per unit area, lower percentage of the tiller ear number/the tiller number, the decreased ear number per unit area, the greatly reduced grain number per ear (1999-2000) and like weight of 1, 000 grains; almost the same plant height; shorter base internode length, a bit more weight per unit internode length, longer internode length under ear; lower LAI values in middle and later stages; coincident process of dry matter accumulation from straight growth onwards, a little more dry matter accumulation amount from earing to maturity; shorter and thicker flag leaf, 2〓 leaf, 3〓 leaf and 4〓 leaf from top, reduced total area of these leave; bigger mean canopy leaf tilt angle, smaller light extinction coefficient , higher ratio of light penetration in the upper, middle and lower parts of the wheat canopy, and tighter plant type; almost identical dynamic change of flag leaf chlorophyll content; and almost the same root amount in 0-120cm soil layer, smaller rate of progressive decrease of the root length density and root weight density with soil getting deep and relatively increased root amount in deeper soil layers.

在传统灌溉-优化施肥条件下，冬小麦单位面积总分蘖数比传统水肥减少，成穗率降低，单位面积穗数有所减少，穗粒数、千粒重无显著性差异；株高基本无变化；基部节间长度缩短，充实度略微增高，穗下节间增长；中后期叶面积指数略低于传统水肥；起身至成熟干物质积累量动态变化过程与传统水肥十分接近，但抽穗至成熟形成的干物质量增加；旗叶、倒2叶倒3叶、倒4叶叶长及总叶面积有所减少，叶片相对增厚；冠层平均叶倾角大，消光系数小，冠层各层的透光率较高，株型紧凑；旗叶叶绿素含量动态变化与传统水肥十分接近；0-120cm土层总根量与传统水肥也差异不大，但根长密度和根重密度随土层深度递减速度较慢，根系在下层土壤的分布相对增多。

Sp. luffae in Taiwan and management of Fusarium wilt of loofah by using 1:3 cleft inarching with resistant cylindrical loofah as rootstock. Fusarium oxysporum f.

sp。 luffae 是否具生理小种分化，以及发展利用抗病圆筒型丝瓜作为根砧，以 1：3 (1株接穗同时嫁接 3 株根砧)割裂靠接法来防治丝瓜萎凋病。

Of all the strains,only strains OBS-2 and 1. 936 of Bacillus subtil is showed significantly inhibiting activity on the above pathogens in dual cult...

在田间小区生防试验中，OBS—2菌株水剂浸根＋OBS—2甘蔗制剂处理38d的防效为59.7％，1.936菌株作同样处理的防效为38.8％，蚀脉尖镰孢水剂是根的防效为41.7％。

The inoculation effectiveness of fungi varied with soil types,especially for BEG167.BEG167 performed positively on cinnamon soil,and neutral on brown soil,but negatively on red soil.Except for BEG150 all All other fungi,except BEG150,increased maize P uptake on cinnamon soil and brown soil,however,only BEG168 and BEG 141 improved P uptake significantly on acidic soil.

若以真菌的根外菌物量作为衡量AM真菌菌株土壤生态适应性的指标，BEG141和BEG167是生态适应性强的菌株，为广幅生态型菌株；菌株BEG168和BEG151次之，前者在棕壤上适应性高，后者在红壤上适应性高。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。