根株

Majority among 39 strains can renodulated on the hosts and on Robinia pseudoacacia ___a legume.

项目分离获得的39株菌株中，多数菌株能回接宿主结瘤，多数回接结瘤的菌株在剌槐的根上也表现出侵染致瘤能力。

We studied the optimum medium and hormone combination of callus induction from cotyledons, subculture of callus, shoot regeneration from callus, shoot-extension induction, root induction and identified the chromosome number of the plantlet. The results showed that: MS+2,4-D 2.0mg/L+KT 0.5mg/L, MS＋2,4-D 2.5mg/L＋KT 0.5mg/L, MS+2,4-D 0+KT 1.0mg/L, MS+KT 0.5 mg/L and basal MS medium. One plantlet among twenty two was determined as a tetraploidy based on the plant morphology, observation of the leaf stoma and chromosome number of the root, other two plantlets represented morphological variation.

结果表明，诱导章丘大葱子叶产生愈伤组织、愈伤组织的继代培养、愈伤组织芽分化、芽点的伸长及诱导生根的最佳培养基及激素配比分别为：MS+2，4-D 2.0mg/L+KT 0.5mg/L、MS＋2，4-D 2.5mg/L＋KT 0.5mg/L、MS+2，4-D 0+KT 1.0mg/L、MS+KT 0.5mg/L和MS基本培养基；试管苗经驯化移栽后，共有22株成活，成活率为92%，对成活的再生植株进行形态学、叶片气孔及根尖染色体鉴定表明，共有3株发生变异，其中2株为外部形态上的变异，而另一株为染色体的加倍变异。

In vitro inoculation technology of Rhododendron fortunei L . with ericoid mycorrhizal fungi and its inoculation effect;2. Observation on Microstructure of Mycorrhizal Root of Rhododendron fortunei L .;3. In this experiment, Rhododendron fortunei L .

本文研究以云锦杜鹃幼苗以及自野生云锦杜鹃根系分离出的菌根真菌为材料，探索云锦杜鹃和菌根真菌形成菌株共生体的最佳接种体系及其菌根真菌和云锦杜鹃的组合效应研究，并根据效应筛选出优良的菌株，为菌根化苗促进生产提供技术基础。

So in our test aantagonistic germ was isolated from ginger soil and was studied. 108 strains of germs were isolated by tube dilution assay from the soil of gingerin Qingmu town,Bazhong city. Q6 strain was screened from these strains of germsthough the first screened and rescreened double with deck agar method andimproved aperture-strikeing method.

本实验从巴中市青木镇堆放腐烂姜并且又长出健康姜苗的根际土中分离出106株细菌，在涂布有姜瘟病原菌青枯劳尔氏菌的肉汤培养基上进行初筛，得到6株对青枯劳尔氏菌有较强拮抗作用的细菌，通过摇瓶发酵后利用发酵液，采用改良的琼脂孔扩散实验复筛，获得一株具有强拮抗作用的菌株，命名为Q6号。

Over 521 strains were screened for phytase production from 30 kinds of soil samples.

从土壤中分离的500多个菌株中筛选出一株产植酸酶活力较高的菌株LW03，经形态学观察和生化试验，初步鉴定为根霉，属于米根霉组。

In bud stage of mustards ecotype Xuelihong and Yuanyejie were transformed with in planta vacuum infiltration by an Agrobacterium tumefaciens strain GV3101 carrying hy-gromycin resistance gene. Effects of different factors on transformation frequency with in planta vacuum infiltration were studied.

以蕾期的芥菜生态型雪里蕻和圆叶芥为试材，在携带潮霉素磷酸转移酶标记基因的根癌农杆菌菌株GV3101的介导下，对影响芥菜植株原位真空渗入遗传转化效果的真空渗入时间、根癌农杆菌悬浮孢子的浓度、渗入培养基、感染方式以及根癌农杆菌菌株等进行研究。

The results showed that in the growth period of R. glutinosa, soil ferulic acid, vanillic acid, and vanillin contents had a persistent increase, while β-hydroxybenzoic acid content increased first but decreased then. The addition of ferulic acid (8 μg·ml-1), vanillic acid (08 μg·ml-1), vanillin (12 μg·ml-1), and β-hydroxybenzoic acid (30 μg·ml-1) in water culture had stronger inhibitory effects on R. glutinosa root length, root mass, fresh plant mass, and plant height. Treating with test phenolic acids except β-hydroxybenzoic acid increased leaf chlorophyll content significantly, compared with the control. After treated with test phenolic acids, plant SOD and POD activities decreased after an initial increase, and plant malondialdehyde content increased. In the treatment of ferulic acid, root SOD and POD activities were the lowest, and roots were rotted and dead.

结果表明：阿魏酸、香草酸和香草醛含量在地黄生长期表现为持续增加，而对羟基苯甲酸含量先升高后降低；外加阿魏酸（8 μg·ml-1）、香草酸（0.8 μg·ml-1）、香草醛（1.2 μg·ml-1）和对羟基苯甲酸（3.0 μg·ml-1）对水培地黄根长、根质量、全株鲜质量和株高有较强的抑制作用；酚酸处理使地黄叶片叶绿素含量降低，除对羟基苯甲酸外差异性极显著；经过酚酸处理的地黄幼苗SOD和POD活性为先升高后下降，丙二醛含量升高，其中阿魏酸的处理使根中酶活性降至最低，根部腐烂死亡。

Soaking seed of rice and wheat with zymolytic liquid of Trichoderma harziaiarum, Trichoderma DWC and Trichoderma viride, comparing with germinate ratio, germinate force, root length, bud length and ratio of root to bud, the results show that 50 times dilute zymolytic liquid of Trichoderma harziaiarum has a relatively notable promote role for germinate of seed .The effect of Trichoderma viride to germinate of seed is not evidence. Trichoderma DWC for seed sprout has certain inhibition.

据此，采用哈茨木霉作为出发菌株，通过离子束诱变，从大菌落株中筛选出促进生长的高产突变株——h-13菌株，通过对用该菌株发酵液处理后的水稻及小麦的种子的各项生理生化指标的测定，发现该菌株比其它菌株更能促进水稻和小麦的活力和发芽率，三天时小麦的总根长为3.03cm，比对照的1.42cm的两倍还多，并且能够明显提高种子发芽后的淀粉酶和蛋白酶的活力，其活力都是对照的两倍左右。

As compared with the traditional irrigation-traditional fertilization, the following results of the improved optimized irrigation-optimized fertilization were obtained: significantly higher nitrogen use efficiency; the like tiller number per unit area, percentage of the tiller ear number/the tiller number, the ear number per unit area, the greatly reduced grain number per ear, and significantly heavier weight of 1, 000 grains; a trifle taller plants; shorter base internode length, a bit more weight per unit internode length, longer internode length under ear; almost the same LAI values in middle and later stages; coincident process of dry matter accumulation from straight growth onwards, a little more dry matter accumulation amount from earing to maturity; a little longer flag leaf, 2〓 leaf, 3〓 leaf (but the three leaves were shorter than those of the optimized irrigation-traditional fertilization) and shorter 4〓 leaf from top, a bit reduced total area of these leaves; almost the same leaf thickness, mean canopy leaf tilt angle, light extinction coefficient , ratio of light penetration in the upper, middle and lower parts of the wheat canopy, and fair plant type; almost the same senescence process of flag leaf; and increased root amount in 0-120cm soil layer and smaller rate of progressive decrease of the root length density and root weight density with soil getting deep, which can strengthen the resistance to adverse conditions such as water stress in the later stage.

在优化水肥条件下，冬小麦总分蘖数、成穗率及单位面积穗数与传统水肥比较接近，但穗粒数显著减少，千粒重显著提高；株高稍有增加；基部节间长度缩短，充实度略微增高，穗下节间增长；中后期LAI与传统水肥的LAI值基本吻合；起身至成熟干物质积累量动态变化过程与传统水肥十分接近，但抽穗至成熟所形成的干物质量增加；上三叶变长（但上三叶长度增加幅度小于优化灌溉-传统施肥处理），倒4叶变短，总叶面积略有减少；叶片厚度、冠层平均叶倾角、消光系数、冠层各层的透光率与传统水肥差异很小，株型较好；旗叶叶绿素含量动态变化与传统水肥十分接近；0-120cm土层的总根量明显增加，上层根群和深层根群的差异最小，有利于提高小麦后期的抗逆力。

Culture medium, water volume, root exudates of different crops, temperature, illumination affecting germination conditions of oospores of Phytophthora sojae studied in this reasch and compared the difference of germination of oospores of different race of Phytophthora sojae, filtrated the higher germination rate of strain and suitbable conditions combination of germination of oospores; The pure-line(Race 1) in virulence was obtained through isolation and culture and virulence identify of the second generation. The pure-line was analyzed by molecule method of ISSR. The results were summarized as follows:There was quite difference of germination rate of oospores on different culture medium.

本文测定了不同培养基、水量、农作物根渗出液、温度、光照对大豆疫霉菌卵孢子萌发影响，比较了大豆疫霉菌株间卵孢子萌发的差异，筛选出萌发率较高的菌株和适于卵孢子萌发的条什组合；通过继代单卵孢株分离培养和毒性鉴定，建立了大豆疫霉菌单卵孢株毒性纯系，并分析了单卵孢株毒性纯系后代群体ISSR分子指纹，结果如下： 1。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。