核酸酶

In Chapter 6, an extensive kinetic study of the inhibition of ribonuclease A and papain by urea has been performed. The results suggest that the effect of urea on activities of these enzymes can be well described by the denaturant binding model. The binding constants of urea determined by the present method are nearly identical to that determined from a variety of different studies on model compounds and proteins.

我们在第六章中研究了脲作用下核糖核酸酶A和木瓜蛋白酶的抑制动力学，发现变性剂结合模型可以很好地描述脲对这两个酶活力的影响，而且用我们自己建立的方法测得的脲与蛋白质的结合常数与前人通过对模型化合物和某些蛋白质的研究所获得的数值基本一致。

More and more attention has been paid to explore effective chemical nuclease with recognition and cleavage of DNA and the new, eff...

寻找有效的、对核酸具有识别与断裂功能的化学核酸酶以及开展抗癌药物设计、发现新的、有效的抗癌药物是我所多年来形成的主攻方向。

A solution of crystalline ribonuclease was injected intraperitoneally andintravenously into the blood stream of the mouse.

核醣核酸酶结晶体的溶液由腹膜腔或血管注射到活鼠的体内可引肝细胞皮质部分的核醣核酸的分解和钙质的消失。

In the same time, using Yang Mai 5 as experiment materials, the effects of ABA and Zeatin on membrane physiology, important photosynthetic parameters, key enzyme of photosynthetic C〓 cycle and proteas, nuclease were also studied.

本文以杨麦5号，杨麦158，麦优4号为材料研究了根系功能和叶片光合功能的变化，对伤流液和根、叶中的ABA、ZRs做了系统的分析测定；以杨麦5号为材料对两种激素在膜生理，重要光合作用参数，光合C〓循环关键酶及蛋白质水解酶，核酸酶的作用进行了研究。

The level of nucleic acid decreased and activities of both nucleases increased are markedly greater in sugarcane leaf protopl...

在细胞壁降解产物的作用下，除了甘蔗原生质体内的RNA酶活性略被促进外，其DNA酶和烟草叶原生质体内的核酸酶均不受影响

In chapter 1, the composition of DNA, the basic mode in DNA binding complexes, current research interesting in this field, as well as major processes made in this thesis are briefly introduced and research methods concerning complex DNA-binding and investigation review about chemical nucleases are the emphases.

第一章前言简要介绍核酸的组成和结构、配合物与DNA相互作用的基本形式以及金属多吡啶配合物与DNA相互作用的研究概况；重点讨论了金属配合物与DNA作用的常用研究方法和有关化学核酸酶的研究进展。

The research on artificial nucleases has been one of the most active frontiers of biochemistry and molecular biology. The investigation on DNA and transition metal complexes as the potential artificial nucleases is the significant branch of bioinorganic chemistry, and is of important theory and application values.

人工核酸切割试剂的研究一直是生物化学和分子生物学最为活跃的研究领域之一，过渡金属配合物作为潜在的人工核酸酶与DNA的作用是生物无机化学研究领域的重要分支，具有很重要的理论价值和应用价值。

DNA, RNA are two basic nucleic acids, DNA, RNA recognition reagents or artificial nucleases are a new challenging filed that is quickly developing in chemical biology.

DNA和RAN是两种最基本的核酸，对DNA和RNA识别试剂或人工核酸酶的研究是近20年化学生物学中发展迅猛、富有挑战性的新领域之一。

This kind of deoxyribonuclease possesses unique characteristics, which is different from the deoxyribonucleases which we have known before.

这种酶具有独特的酶学性质，不同于以往所知的脱氧核糖核酸酶。

The purification method of the deoxyribonuclease comprises the following steps: drying fresh fruiting body of Ganoderma lucidum, pulverizing, extracting to obtain crude deoxyribonuclease, salting out with ammonium sulfate, carrying out affinity chromatography, gradient-eluting, carrying chromatography in SP Sephorose Fast Flow column, and gradient-eluting with 0 to 0.3 M NaCl to obtain seoxyribonuclease of Ganoderma lucidum.

该酶的提取纯化方法，以新鲜灵芝子实体为原料，经干燥处理后粉碎、粗酶抽提、硫酸铵盐析、亲和层析，梯度洗脱，再用SP Sephorose Fast Flow柱层析，以0～0.3M NaCl梯度洗脱，即可获得灵芝脱氧核糖核酸酶。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。