核酸酶

The royal jelly is the secretion from the bee milk gland of young worker, which is a serum substance, with sour, astringent and piquancy tastes, and which is the food for queen bee and early little bee.

蜂王浆是青年工蜂上颚腺分泌物，味辛酸，性微温，富含有人体所需的蛋白质、氨基酸、乙酰胆碱、维生素、酶、微量元素、生长激素、核酸与蛋白多肽等生物活性物质。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

What is the function of ligase in DNA replication?

什麽是连接酶的作用在脱氧核糖核酸复制的？

The number of 5-Br deoxy uridine monophosphate and nestin in subventricle zone and dentate gyrus of rats at 3, 5, 7, 14, 21 and 30 days after MCAO were detected by immunohistochemistry and the bFGF mRNA were detected by RT-PCR at the same time.

免疫荧光法检测各组大鼠缺血再灌注损伤后第3、5、7、14、21、30天病灶侧侧脑室下区、海马齿状回区5-溴脱氧尿嘧啶核苷和Nestin的变化，逆转录聚合酶链式扩增方法检测相应时间点病灶侧碱性成纤维生长因子信使核糖核酸的表达。

These data suggest that the terminal hairpin structure and ITRs of PfDNV play a key role in the rescue and gene correction process: sequence at the junction of the terminal palindrome of PfDNV genome and the pUC119 can form typical Holliday structure, it can be cutted by the special endonuclease, then the exposed 3'hairpin structure serving for a self-primer to initiate viral DNA replication; and the intact extremity acting as a template to repair another altered end within inverted terminal repeats.

说明存在着一种拯救和基因修复机制，推测与PfDNV基因组末端结构（Hairpin、ITR）有密切关系：PfDNV基因组与pUC119质粒连接处可形成Holliday结构而被识别该结构的核酸内切酶所切割，游离下的基因组3'发夹结构作为引物起始复制，然后被置换链的完整的5'ITR作为模板修复部分缺失的另一端ITR，推测这个过程需要病毒NS蛋白以及细胞因子的参与。

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锌--使皮肤酶素发挥各种作用，从而制造核糖核酸，帮助伤口愈合，加强免疫功能

In this study, multiple primer polymerase chain reaction , consensus primer PCR and in situ hybridisation with biotin-labelled DNA probe (using domestically-produced agents) were applied and the sensitivity, specificity as well as practical value of each method were compared.

本实验采用了国内外领先的多重聚合酶链反应（polymerase chain reaction，PCR）和共用引物PCR法及国产生物素标记探针原位核酸杂交法，比较和估价了三种方法的灵敏度，特异性和在实际工作中的应用价值。

Hepatitis B virus ; Breast-feeding ; Polymerase chain reaction; Deoxyribonucleic acid

乙型肝炎病毒；母乳喂养；聚合酶链反应脱氧核糖核酸

The answer was that the former could be far sensitive than the latter, but the former needed complex instruments. The latter could help us to directly judge the order of severity by colors.On the other hand, we took advantage of polymerase chain reactionto amplify the MCP genome of the virus and compare it with other nine published MCP genomes of viruses including CzIV, TIV, CIV, LCDV-1, LCDV-K1, BIV, FV3, GIV, RSIV. The sequencing data showed that the MCP of Cobia iridoviruswas more homologous to the MCP of LCDV-1（89﹪similarity）than that from FV3（47﹪similarity）.

另一方面，为了了解引起海鲡病变之病毒究竟属於何种病毒，我们透过聚合酶链锁反应增幅其主要鞘蛋白基因并加以定序，与其他九种已发表的虹彩病毒之 MCP 核酸及胺基酸序列，包括：CzIV、TIV、CIV、LCDV-1、LCDV-K1、BIV、FV3、GIV、RSIV 之 MCP 进行分析比对，显示 Cobia iridovirus亲缘性和感染大多数硬骨鱼类的 Lymphocystivirus 属的 LCDV-1 最为接近（89﹪相似度），和感染两栖类的 Ranavirus 属的 FV3 亲缘性较低（47﹪相似度）。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。