核酸酶

The macrocyclic polyamines are a kind of excellent ligands and have a substantially high binding affinity with transition metal ions. Macrocyclic polyamine metal complexes as enzyme models are widely used in chemical biology, medicine and gene therapy. 1, 4, 7, 10-tetrazacyclododecane is a representative macrocyclic polyamines compound and shows high binding affinity with transition metal ions, such as Zn, Cu and Co. Their metal complexes especially multinuclear complexes were widely used in DNA, RNA recognition.

大环多胺化合物是一类性质独特的优秀配体，具有良好的金属配位能力，其金属配合物作为人工酶模型广泛应用于核酸结构及功能研究、基因表达与调控、抗癌药物的研制等生物、化学和医学领域。1，4，7，10-四氮杂十二烷是典型的大环多胺化合物，对过渡金属离子Zn， Cu and Co有良好的配位能力，这些金属配合物特别是多核金属配合物被广泛应用于DNA识别中。

Methods Serological test and PCR method were used to detect 117 of 15 species of wild animals and 99 domesticated Paguma larvata .

对广西境内15种117只野生动物及99只人工养殖的果子狸采用聚合酶链反应和血清学方法检测其病毒核酸。

According to the alignment of Blastn and Blastx, one differentially exp ressed fragment, A7, was 97% identical in nucleotides to the 26S ribosomal RNA gene of Photinia fraseri and 91% in amino acids to cytochrome P450 monooxygenase in maize, respectively. Another differentially exp ressed fragment, A16, was 85% in nucleotides and 93% in amino acids identical to alpha-expansin 2 in Triphysaria versicolor.

核酸和氨基酸比对分析中同源性最高的是片段A7和A16.A7的核苷酸序列同源于红叶石楠Photinia fraseri 26S核糖体RNA (97%)、蛋白同源于玉米的细胞色素P450单加氧酶(91%)， A16的核苷酸(85%)及蛋白(93%)同源于直果草属 Triphysaria versicolor 的α-expansin 2基因。

Using real-time reerse transcriptase polymerase chain reaction, transforming growth factor- messenger ribonucleic acid expression was quantitatiely measured.

通过实时逆转录聚合酶链反应，定量测定转化生长因子信使核糖核酸的表达。

The CORNELL/COPERSUCAR/USPA project for the application of RELP technology in sugarcane encompasses three objectives: aEstablishing measures of genetic distance among and within species of the Saccharum complex and hybrids. bDevelopment of a genetic linkage map applicable to the saccharum genera.

CORNELL/COPERSUCAR/USPA 合作的多倍体的核酸内切酶内切限制断段变化技术应用于甘蔗，包括三个目的：a建立甘蔗属的合成物和杂种种内和种间的基因距离的测定。b发展应用于甘蔗属的基因链锁图。c努力建立分子标记和农学特性之间的链锁。

Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.

采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型，同时以何首乌饮灌胃作为延缓衰老组；造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况；测定各组Fas和FasL的信使核糖核酸水平；SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。

When food is in the presence of Y radiation, ionisation occurs within the cell nuclei.

食物受到γ射线照射时，可与体内细胞发生电离作用，电离产生的离子能侵蚀复杂的有机分子，如蛋白质、核酸和酶。

An enzyme that catalyzes the formation of DNA and RNA from an existing strand of DNA or RNA.

一种催化DNA或RNA的酶，利用一段既成的DNA或RNA作为核酸的模子。

The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

分离解剖耳蜗后，用碘化丙啶染色细胞核、pholloidin染色f-actin，免疫荧光抗体分别染色单链dna、核酸内切酶g和凋亡诱导因子（apoptosis inducing factors， aif），制备耳蜗铺片，激光共聚焦显微镜下观察凋亡和坏死毛细胞内的荧光信号变化。

Bioinformatics analysis showed that over 150 AP2 genes in Arabidopsis could be divided into five subfamilies on the basis of number of repetition and sequence of AP2 domain.

AP2基因曾被认为植物所特有，但最近在蓝藻、线虫和病毒中发现了具有AP2结构域和位点特异核酸内切酶的蛋白。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。