核酸酶

Objective To test the in vitro effect of daphnetin on cytochrome C oxidase and ribonucleotide reductase activity of Plasmodium falciparum.

目的 体外测定瑞香素对恶性疟原虫细胞色素C氧化酶及核糖核酸还原酶活性的影响。

Methods Biopsy samples of were obtained from 353 patients with cervical carcinoma, HPV were detected by PCR and endonuclease cleavage. Results The results indicated that HPV types 16, 18 were found in 138 (39.1%) 353 cervical carcinoma, while HPV types 6, 11 were found in 81 (69.2%) of 117 acuminate verruca.

采用聚合酶链反应-核酸内切酶分型检测宫颈癌活检组织中HPV-DNA，对来源于子宫颈癌患者的石蜡包埋组织标本进行四种常见生殖道人乳头瘤病毒(HPV6、11、16、18)的检测和分型。

Methods Trizol reagent from clamworm digestive tract epithelial cells extracted methods Total RNA. The mRNA was purified and reverse transcripted into cDNA. The cDNA was linked into plasmid pDNR-Lib after digested by nucleate endonuclease Sfi I to construct cDNA library.

分离沙蚕消化道上皮细胞，使用Trizol试剂提取总RNA，纯化mRNA，用MMLV逆转录成cDNA，用Sfi I核酸内切酶酶切后连接到pDNR-Lib质粒中，构建cDNA文库。

Objective To test the in vitro effect of daphnetin on cytochrome C oxidase and ribonucleotide reductase activity of Plasmodium falciparum . Methods P.

摘要目的体外测定瑞香素对恶性疟原虫细胞色素C氧化酶及核糖核酸还原酶活性的影响。

Daphnetin significantly reduces the COX and RNR activities of Plasmodium falciparum in vitro .

在体外瑞香素可显著降低恶性疟原虫的细胞色素C氧化酶及核糖核酸还原酶活性。

The inhibition level of the COX activity by daphnetin after being treated for 2, 4, 8 and 12 h were 0.6%, 73% and 80% respectively and the inhibition level by daphnetin at different concentrations （0.1, 1, 100 and lmmol/L） for 6h was 3%, 31%, 53% and 84%, respectively.

在体外瑞香素可显著降低恶性疟原虫的细胞色素C氧化酶及核糖核酸还原酶活性。

Methods The system employs the 5' 3' nuclease activity of Taq DNA polymerase to digest a salmonella specific internal fluorogenic probe which is labeled with two fluorescent dyes and hybridizes to the target DNA during PCR.

利用TaqDNA聚合酶的 5' 3'核酸外切酶活性降解特异性杂交于模板上的荧光标记探针，导致荧光强度的改变，通过检测荧光强度分析PCR扩增产物。

With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase , Nucleic acid-binding protein , Ribosomal protection tetracycline resistance protein , hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase .

应用PMF技术对这11个具有显著表达差异蛋白点中的9个进行鉴定，得到了5个质谱阳性鉴定结果，其中2个蛋白分别为玉米种属的1，5-二磷酸核酮糖羧化酶／加氧酶和叶绿体的核酸结合蛋白；其它的3个蛋白在玉米蛋白质数据库中未见报道，但与其它物种基因编码的蛋白质具有高度的序列同源性。

As well as nucleic acid, amino acid, gland glucosidal, each kind of enzyme and its coenzyme, polysaccharide synthesis essential element.

富含有机酸和生物促长剂，提供可直接被草株吸收的N、P、K等营养形态，以及核酸、氨基酸、腺苷、各种酶及其辅酶、多糖合成的必需元素。

Gene sequence was highly homologic with a gene encoding aβ- subunit of ATP synthetase and an EST originating from a cDNA in relation to water deficit stress, which both were closely associated with the protein functioning in stress resistance and ion transportation. The results suggested that No. 1 gene sequence was involved in stress resistance of apple rootstock. No. 2 gene sequence had high homology with a gene encoding a serine/ threonine kinase protein, indicating perhaps it was a new gene encoding the serine/ threonine kinase protein in apple rootstock. The gene sequences of A1 and A2 were highly homologic with a gene encoding aβsubunit of ATP synthetase.

对得到的差异片断进行回收、测序，通过BLAST和其它植物比对，发现Ⅰ号序列与一条编码ATP合酶β亚基基因和一条水分亏缺胁迫cDNA克隆基因EST的同源性非常高，该序列编码涉及到了抗性表达、离子转运基因，可能参与了植物抗性表达，说明获得的基因序列应是与苹果砧木抗性相关的基因；Ⅱ序列与一条serine/threonine kinase蛋白激酶的蛋白序列同源性比较高，核酸比对较低，可能是具有丝氨酸/苏氨酸蛋白激酶功能的新基因。A1、A2序列与一条编码ATP合酶β亚基基因的同源性较高。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。