核酸酶

The members with Leber hereditary opˉtic neuropathy have a point mutation in mtDNA and the mutation at np11778is one of mechanisms inducing this disease.PCR technique provides a useful diagnostic aid for LHON.

该LHON家系成员存在线粒体DNA点突变，mtDNA的ND4亚单位第11778核苷酸突变是本病的发病机制之一，PCR-限制性核酸内切酶酶谱分析技术为LHON的诊断提供了依据。

Activity of Diamine oxidase: DOA is the highly active endoenzyme in the microvilli of small intestine of human and other mammal, which activity has crucial correlation with nucleic acids and protein synthesize within mucous cells and also reflects the completion or trauma level of small intestinal mucosa.

二胺氧化酶活性：DAO是人类和哺乳动物小肠粘膜上层绒毛中具有高度活性的细胞内酶，其活性与粘膜细胞的核酸和蛋白合成密切相关，可反映小肠粘膜的完整性和损伤程度。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

The Nucleonics' HBV clinical candidate encodes four short hairpin RNA molecules, each under the control of an RNA polymerase III promoter.

该nucleonics的乙型肝炎临床候选四个编码短发夹核糖核酸分子每个控制下的一种核糖核酸聚合酶三子。

As compared with the tissue of young leaf, the contents of RNA, DNA and total nucleic acid in freshly isolated protoplasts from sugarcane Saccharum officinarum L.

与幼叶组织相比，酶法新鲜分离的甘蔗和烟草幼叶原生质体内的RNA、DNA及总核酸含量均降低。其原因可能是刚游离的原生质体内酸性和碱性RNA酶与DNA酶等活性提高所致。

The Center for Advanced Biomolecular Research is primarily dedicated to education and research in biomolecular including mechanism and regulation of enzymes, Protein folding and stability, proteomics, enzyme regulation and design, mechanisms of enzyme allostery, nuclear magnetic resonance, structural biology, proteins and RNA folding thermodynamics.

高级生物分子研究中心主要致力于生物分子的教学与研究，其研究领域包括酶作用机理，蛋白折叠和稳定性，蛋白组学，酶调控和设计，酶变构机制，核磁共振，结构生物学，蛋白质和核糖核酸折叠热力学等。

At the computers heart is a ribozyme – a short RNA molecule able to catalyse changes to other molecules.

计算机的核心是酶性核酸――一类具有催化断裂RNA作用的核酸分子。

What is the function of DNA polymerase in DNA replication?

什麽是脱氧核糖核酸聚合酶的作用在脱氧核糖核酸复制的？

Acquiring real time process information of interactions between nucleic acids and polymerases would not only benefit to understand mechanism of DNA replication, but also provide a new research tool for drug screening.

核酸的复制是生命现象中最本质的内容之一，获取有关核酸和聚合酶作用的实时过程信息将有助于DNA复制过程机理的研究，而且还将为药物的筛选提供新的技术手段。

Methods In 50 cases of patients after treatment of artificial kidney and 54 healthy volunteers, the prevalence of HHV-7 in peripheral blood mononuclear cell and cell-free viral DNA in plasma was tested by nested polymerase chain reaction assay; the product of PCR was digested by restricted enzyme EcoR I to identify its specificity.

采用巢式聚合酶链反应对50例肾透析病人和54例正常健康人PBMC和血浆标本中HHV-7 DNA进行定性检测；用限制性核酸内切酶EcoRⅠ对PCR产物进行酶切图谱分析以确定扩增产物的特异性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。