核酸酶

The efficacy of this technique for protein mapping was demonstrated by the mass spectral analyses of the peptide fragmentation of several proteins, including lysozyme, albumin, conalbumin, and ribonuclease A.

我们利用四种蛋白质，溶菌蛋白、白蛋白、伴蛋白和核醣核酸酶A的胜肽片段之质谱分析而证明这个方法是非常有效率的。

Positive rate was relatively high, and the foreign gene could also maintain in testicular tissue for a long time, it could still be detected in the tissue in 7 months after being injected. However. Deferent results could be gained when the detection were made with different primers on different sites of the recombinant plasmid. This could be explained by transgene lost when the foreign gene recombinated and integrated in heterogenetic cell, or transgene degradated by cell nucleases.

然而，我们也看到，在后代检测中，利用不同的引物扩增重组质粒不同部位时，检测结果存在差异，转基因阳性率不同，这种差异说明当外源基因进入到异源细胞内，在重组整合时发生了外源基因丢失的现象，并由此导致外源基因的整合率下降，也可能是受细胞内核酸酶的作用，使得外源基因遭到降解。

We have investigated the conformational features of four short peptide fragments of Staphylococcal nuclease by homonuclear two-dimensional NMR and circular dichroism with a view to examining the early events in the folding process of SNase.

本论文工作用四个金黄色葡萄球菌核酸酶N-末端短片段作为新生肽链折叠的模型，用同核二维核磁共振谱和远紫外圆二色谱研究了它们的溶液构象特征。

Zn~(2+), iminazole, EDTA, K+, Cu~(2+)could inhibit its activity respectively.

确立了核酸酶粗提物的琼脂糖凝胶电泳测活体系。

Objective To study partial biochemical characterization and effect of lumbricus dornases.

：目的研究地龙体内两种新型脱氧核糖核酸酶的部分生化性质，并对其作用进行初步探索。

Exploring the Structures, Coding Genes and Characteristics of Lumbricus Bimastus Nucleases EWD_1,2,3

双胸蚓组织中核酸酶EWD_（1，2，3）的结构、基因和相关性质研究在线阅读整本下载分章下载分页下载

A preliminary study on substrate specificity of Lumbricus Bimastus nuclease.

双胸蚓组织核酸酶底物特异性的初步研究方法1。

A preliminary study on substrate specificity of Lumbricus Bimastus nuclease DNase E degradation studies showed that there were differences in degradation rate of the DNase E to different substrates.

双胸蚓组织核酸酶底物特异性的研究通过DNase E在最适反应条件下对几种不同底物降解速率的研究表明DNase E降解底物存在差异性。

A preliminary study on substrate specificity of Lumbricus Bimastus nucleaseTo measure degrade rate of DNase E to several different substrate in optimal reaction condition to demonstrate the substrate selectivity of DNase E. Agarose gel electrophoresis was used to investigate the products of DNase E processing double strands cyclic DNA to investigate the action of DNase E.

双胸蚓组织核酸酶底物特异性的研究测定DNase E在最适反应条件下对不同底物的降解速率，证明DNase E降解底物的选择性；应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定，阐明DNase E的水解方式。

The spheroplast suspension (200 ul) was transferred into a microcentrifuge tube, supplemented with 150–350 units/ml micrococcal nuclease (Fer- mentas), and incubated at 37°e for 5 min.

暂停的原生质（ 200 UL ）的是转入微量管，补充与150-350单位/毫升微球菌核酸酶，并培养在37 ° E的5分钟。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。