核菌

Effect of tenuazonic acid on micronucleus and karyokinesis of Vicia faba root tip cells was investigated to evaluate its cytogenetic toxicity.

利用蚕豆根尖为材料，研究了链格孢菌毒素细交链孢菌酮酸对细胞微核和有丝分裂的影响，以评价其细胞遗传毒性。

The results indicated that when its concentration was 12.5~50μg/mL, no significant effect of tenuazonic acid on micronucleus ratio and karyokinesis index was found compared with control, the micronucleus ratio was less than 3‰ and karyokinesis index was more than 9.5%.

结果表明，当浓度为12.5~50μg/mL时，细交链孢菌酮酸对蚕豆根尖细胞微核率和有丝分裂指数不产生显著影响，微核率低于3‰，有丝分裂指数超过9.5%。

However, as the concentration increased to 100~400μg/mL, the micronucleus ratio significantly rose, while the karyokinesis index significantly decreased.

浓度升高到100~400μg/mL时，细交链孢菌酮酸使蚕豆根尖细胞微核率显著升高，有丝分裂指数显著下降，其中，400μg/mL时，微核率高达36.01‰，有丝分裂指数仅为1.90%。

The present invention discloses beet noctuid nuclear polyhedrosis virus insect killing suspending agent, in which the virus bearer content is 5-20 hundred million PIB/ml., itis composed of chlorbenzuron analogue chlorfluazuron, aromatic amino acid tyrosine, fluorescent whitener, dispersant, glycerin, sterilized water.

本发明公开了一种甜菜夜蛾核多角体病毒杀虫悬浮剂。该剂型的主要成分是甜菜夜蛾核多角体病毒，悬浮剂中病毒包涵体含量5－20亿PIB/ml，它由灭幼脲类似物氟啶脲、芳香族氨基酸酪氨酸、精制荧光增白剂、分散剂、甘油、无菌水组成。

The plant Quarantine Lab had intercepted more than 200 quarantine pests such as : Tilletia indica , Tilletia controversa , Bursaphelenchus xylophilus , sorghum halepense , Acanthoscelides obtectus , Sternochetes mangiferae , Bactrocera dorsalis , Coptoermes curvignathus for nearly 5000 batches ; The Animal Quanantine Lab had intercepted about 20 cow which had some pathogens evidence of infectious disease such as : bovine infectiouse rhinotracheitis , leukaemia bovum , paratuberculosis and so on ; The food microorganism lab had detected over 300 unqualified products such as Salm-Sury , Listeria monocytogenes , Mycobacterium paratuberculosis etc .

多年来，分中心各实验室切实履行严格把关的职责，植物检疫共截获大豆疫霉病菌、小麦印度腥黑穗病菌、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、芒果果核象甲、桔小实蝇、大家白蚁等国家禁止进境的危险性有害生物220 多种近10000 批次；动物检疫发现感染牛传染性鼻气管炎、牛白血病、副结核等传染病的疫牛20 多头；食品微生物检测共检测沙门、单增、副结核的不合格商品300 多批；分子生物学实验室为国内企业出具非转基因证书和不含有牛羊源成分证书50 余份，使相关的产品能够顺利出口到欧盟、韩国和日本等国。

The results showed that the biological activities are closely related to the steric conformation and distribution of electrostatic potential of carboxyl group: The coplanatity between the group of C-3 position and the parent nucleus and the coplanatity between the group of C-3 position and C-4 keto group are very important for biological activities.

结果发现该类药物的抗菌活性与C 3位的空间构象和静电势分布有着紧密的联系。C-3位羧基与C-4位酮基共面性、以及它和母核共面性对抗菌活性十分重要，C-3位两个氧原子周围较强的负静电势也是影响活性的重要因素。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Results Total positive rate was 80.4%, the mold detection rate was 28.6%, the condition pathogenic bacteria 28.3%, vagina Gartner the fungus 15.8%, the infusorium 13.1%, the gonococcus 3.8%, the filament trichobacteria 2.0%, the nuclear neterogeny cell 0.5%, the multiple infections account for 12.6%; This method compared with saline smear detection rate has increased significantly.

结果 总阳性率为80.4%，其中霉菌检出率为28.6%，条件致病菌检出率为28.3%，阴道加特纳菌检出率为15.8%，滴虫检出率为13.1%，淋球菌检出率为3.8%，纤毛菌检出率为2.0%，核异质细胞为0.5%，多重感染占12.6%；此法与盐水涂片法相比检出率有显著性提高。

The mutants SV-5-1, SV-5-2 and SV-10-1 were effective in suppression of the infection of rapeseed leaves by ascospores of S.

突变菌株与野生菌株在油菜花瓣上对核盘菌子囊孢子侵染油菜叶片具有明显抑制作用。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法，测定西帕依固龈液对牙龈卟啉单胞菌（Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度；对10例志愿者采用自身前后对照试验，观察四种干预措施（无干预、清水、西帕依固龈液、口泰）对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响，评价西帕依固龈液防治口臭的效果。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐