核菌

Objective To construct recombinant plasmid of Legionella pneumophila mip gene and detect its expression in NIH3T3 cells.

目的 构建嗜肺军团菌mip基因的真核表达重组质粒pcDNA3.1-mip，并研究其在细胞中的表达。

This study has built a foundation for the development of PAL gene DNA vaccine for Legionella pneumophila.

成功构建了真核表达重组质粒PcDNA3.1-PAL为嗜肺军团菌核酸疫苗的进一步研究奠定了基础。

According to the sequence of rabbit defensin NP-1 and porcine defensin pBD-1 gene published in GenBank,We selected respectively prokaryon or eukaryotic expression vector to construct recombinant expression plasmid hoping for getting expression protein in E.coli or COS-7 cells, All these laid down a foundation in research of these two antibacterial peptides.

本文主要根据GenBank已公布的兔防御素NP-1基因和猪防御素pBD-1基因序列，分别选用原核或真核高效表达载体构建重组表达质粒，以期在大肠杆菌或COS-7细胞中得到高效表达，为进一步研究这两个抗菌肽奠定基础。

The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Co. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin in liver tissues of all mice.

采用60Co治疗仪γ射线对实验组和对照组行350cGy的亚致死剂量照射，实验组A、B、C3组照射后3h内经尾静脉分别输入1.0×10^7个/只、2.0×10^7个/只和3.0×l0^7个/只人新鲜脐血有核细胞，对照组经尾静脉注入等体积无菌生理盐水。1个月后对实验A、B组和对照组裸鼠经腹膜腔注射四氯化碳(CCl4)，实验C组注射等体积生理盐水。2个月后采用免疫组化和RT-PCR方法检测裸鼠肝组织人源性CK18、CK19和人源性白蛋白的表达。

Under the support of NSFC, 3 bioactive nucleoside antibiotics against agricultural pathogenic fungi were purified from fermentation broth of DKL producing strain.

本项目从多抗灵产生菌的培养物中主要分离了三个有抗真菌活性的核苷类化合物(DKL-A；DKL-B， DKL-C)。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。