核菌

Objective To construct recombinant plasmid of Legionella pneumophila mip gene and detect its expression in NIH3T3 cells.

目的 构建嗜肺军团菌mip基因的真核表达重组质粒pcDNA3.1-mip，并研究其在细胞中的表达。

This study has built a foundation for the development of PAL gene DNA vaccine for Legionella pneumophila.

成功构建了真核表达重组质粒PcDNA3.1-PAL为嗜肺军团菌核酸疫苗的进一步研究奠定了基础。

According to the sequence of rabbit defensin NP-1 and porcine defensin pBD-1 gene published in GenBank,We selected respectively prokaryon or eukaryotic expression vector to construct recombinant expression plasmid hoping for getting expression protein in E.coli or COS-7 cells, All these laid down a foundation in research of these two antibacterial peptides.

本文主要根据GenBank已公布的兔防御素NP-1基因和猪防御素pBD-1基因序列，分别选用原核或真核高效表达载体构建重组表达质粒，以期在大肠杆菌或COS-7细胞中得到高效表达，为进一步研究这两个抗菌肽奠定基础。

The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Co. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin in liver tissues of all mice.

采用60Co治疗仪γ射线对实验组和对照组行350cGy的亚致死剂量照射，实验组A、B、C3组照射后3h内经尾静脉分别输入1.0×10^7个/只、2.0×10^7个/只和3.0×l0^7个/只人新鲜脐血有核细胞，对照组经尾静脉注入等体积无菌生理盐水。1个月后对实验A、B组和对照组裸鼠经腹膜腔注射四氯化碳(CCl4)，实验C组注射等体积生理盐水。2个月后采用免疫组化和RT-PCR方法检测裸鼠肝组织人源性CK18、CK19和人源性白蛋白的表达。

Under the support of NSFC, 3 bioactive nucleoside antibiotics against agricultural pathogenic fungi were purified from fermentation broth of DKL producing strain.

本项目从多抗灵产生菌的培养物中主要分离了三个有抗真菌活性的核苷类化合物(DKL-A；DKL-B， DKL-C)。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐