核菌

Chemotaxis is involved in the whole life cycle of Dictyostelium, a simple social amoeboid organism displaying chemotactic behavior very similar to that observed in higher eukaryotic cells.

黏菌的生活史中，从生长期的吞食细菌到发育期的聚集与型变，趋化运动都参与其中，且无论在行为上或牵涉的分子都与高等真核细胞非常相似。

A binucleate spore of a rust fungus , formed in a chainlike series in an aecium .

锈孢子锈菌的双核孢子，在锈孢子器中形成系列来源：词友

The analysis in this study provided useful information on applications of microsatellites in population genetics of S. sclerotiorum and B. cinerea .

文章对核盘菌和灰葡萄孢基因组中SSRs的丰度、出现频率及最长基序的分析为快速、便捷地设计多态性丰富的SSRs引物提供了有益的信息。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

In the more advanced fungi, especially the Basidiomycota, nuclei may fuse some considerable time after fusion of hyphae of different mating strains, forming a dikaryon.

在较高等的真菌中，尤其在担子菌门中，不同类型的菌丝融合后细胞核需要较长的时间进行融合，从而形成双核体。

The non-volatile substances of Trichoderma have the obvious inhibitions to the hyphal growth, number and size of sclerotia of S.sclerotiorum.

木霉的非挥发性代谢产物对核盘菌菌丝的生长、菌核的数量和大小有明显影响。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

Using the intact G1-nuclei prepared from an isogenic yeast strain lacking mitochondrial DNA , we demonstrated that the DNA synthesis observed within the first 10-20 min was largely due to mitochondrial DNA synthesis, whereas nuclear DNA synthesis did not begin until after a 10-20 min lag period.

利用从缺少线粒体DNA的菌株中制备的完整G1期细胞核，证明了最初10-20min的滞后期内的DNA合成主要是由线粒体DNA合成造成的，而核DNA合成是在经过10-20 min后才开始。

Micrococcal nuclease hypersensitive DNA fragments were either cloned directly, or mapped onto the genome by hybridization with oligo-nucleotide probes on a 244K Agilent array-CGH chip. The array-CGH mapping was confirmed for nuclease sensitivity by PCR after MNase digestion and by Southern blot analysis.

我们利用小球菌核酸酶超敏感的去氧核醣核酸片段直接选殖或是将其作为寡核苷酸探针於安捷伦244k寡核苷酸阵列-比较基因体杂合晶片上，进行基因体定位，并利用聚合酶链索反应和南方墨点法侦测其核酸酶敏感程度，以确认结果之可靠性。

By June of 2008, there were less than 80 fungal genomes that were completely or almost completely sequenced and publicly accessible representing the widest sampling of genomes from any eukaryotic kingdom. These genomes belonging to true fungi, Microsporidia and Oomyces vary in size from approximately 2.5 Mb to 81.5 Mb.

至2008年6月，近80种隶属于真菌、微孢子虫和卵菌的全基因组序列公布，代表着最广泛的真核生物，它们的基因组大小从2.5 Mb~81.5 Mb。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐